Interleukin-6 in Tear Fluid After Photorefractive Keratectomy and Its Effects on Keratocytes in Culture

Malecaze, F; Simorre, V; Chollet, Philippe; Tack, J L; Muraine, M.; Guellec, Dominique Le; Vita, N; Arné, J L; Darbon, J M

doi:10.1097/00003226-199709000-00016

Cited by 39 publications

(21 citation statements)

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“…IL-6 may also play a crucial role in the development of postoperative infl ammation after cataract surgery [16] , since it has been shown to stimulate collagen synthesis and may therefore be involved in corneal wound healing [9,10,17] . IL-6 is a pluripotent cytokine: besides its infl ammatory role, it is also part of an endogenous anti-infl ammatory system [12] .…”

Section: Enhanced Release Of Il-6 and Il-8 Into Tearsmentioning

confidence: 99%

Enhanced Release of IL-6 and IL-8 into Tears in Various Anterior Segment Eye Diseases

et al. 2006

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Objective: To determine the levels of interleukin 6 (IL-6) and interleukin 8 (IL-8/CXCL-8) in tears collected from the eyes of normal individuals and of patients with different irritative eye diseases, in order to acquire information on the immunological changes occurring during the early postoperative period following various forms of eye surgery, including penetrating keratoplasty (PKP). Methods: IL-6 and IL-8 levels were measured with the aid of human ultrasensitive ELISA kits in the non-stimulated tears of patients in the early postoperative period following PKP or cataract operation, and of patients with acute bacterial conjunctivitis or with a corneal foreign body. The IL-6 and IL-8 concentrations, the total amounts released in a given time and the rates of their release were calculated. Results: A significant increase in IL-6 release was observed in all patient groups compared with the normal controls (p ≤ 0.003). The IL-8 release levels were significantly higher in the tears of all patient groups (p ≤ 0.03), except for the cataract operation group, where the IL-8 release was not significantly higher (p = 0.053) than in the control samples. No significant differences in IL-6 or IL-8 release were observed when the various patient groups were compared with each other. Conclusion: The release of IL-6 and IL-8 into the tears is enhanced in various anterior segment eye diseases, and this may be used as an indicator of various inflammatory reactions in the early postoperative period.

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Section: Enhanced Release Of Il-6 and Il-8 Into Tearsmentioning

confidence: 99%

Enhanced Release of IL-6 and IL-8 into Tears in Various Anterior Segment Eye Diseases

et al. 2006

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“…Dead cells are removed by macrophages and damaged extracellular matrix by metalloproteinases (Ye and Azar, 1998;Ye et al, 2000) and the plasmin system (Berman et al, 1980). Both processes are regulated by several cytokines synthesized and released by the epithelium, i.e., transforming growth factor (TGF)-b and interleukin 1 and 6 (Girard et al, 1991;Malecaze et al, 1997). The synthesis of new tissue requires proliferation, migration, and differentiation of epithelial cells, which are stimulated by growth factors secreted by keratocytes, epithelial growth factor (EGF), keratinocyte growth factor, and hepatocyte growth factor (Klenkler and Sheardown, 2004;Wilson et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Wound healing following refractive surgery in hens

Martínez-García¹,

Merayo‐Lloves

Blanco–Mezquita

et al. 2006

Experimental Eye Research

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The wound-healing response is critical to the outcome of refractive surgery and studying wound healing contributes to an understanding of the pathophysiology of other corneal injuries. Animal models allow research to be conducted with sufficient samples and under controlled parameters. We studied the hen to determine the healing process from clinical, biophysical, and biological standpoints after photorefractive keratectomy (PRK). PRK (ÿ6.0 diopters) was performed in hen eyes. At 3, 6, 12, 24, 48, and 72 h and 5, 7, 15, 30, and 60 days postoperatively, we studied the clinical follow-up, objective measurements of light transmission (direct transmittance), apoptosis by TUNEL assay, proliferation by immunocytochemical analysis of 5-bromo-2 0 -deoxyuridine, and expression of alpha smooth muscle actin (SMA) in myofibroblasts in the corneas. Hen corneas reepithelialize quickly. Haze developed from 5 to 60 days after surgery and was correlated with the appearance and finalization of the expression of SMA. The direct transmittance of light was low during the first 15 days and improved at 30 and 60 days. TUNEL-positive cells were observed 3 h after surgery and the numbers decreased thereafter. Epithelial proliferation began at 12 h and was greater at 48 h, while stromal cell proliferation began at 24 h and was greater at 72 h. The hen cornea is anatomically similar to the human cornea, and the manner in which it heals is a good model for studying different surgical techniques and pharmacologic assays.

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“…Little evidence shows any changes to the lipid or mucin cell layers of the tear film after LASIK. Few studies have shown the direct effects of tear film cytokines after LASIK, such as increases in transforming growth factor-b1 (TGF-b1) concentration after epi-LASIK [10] as well as tumor necrosis factor-a (TNF-a) [11], interleukin (IL)-6 [12], Vascular Endothelial Growth Factor (VEGF) [13], platelet derived growth factor (PDGF)-BB [14], and hepatocyte growth factor [15] after PRK. Interestingly, however, ascorbic acid levels in the tear film are lower after LASIK.…”

Section: Tear Changesmentioning

confidence: 99%

Allergic complications with laser-assisted in-situ keratomileusis

Bielory

O’Brien

2011

Current Opinion in Allergy &Amp; Clinical Immunology

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LASIK surgery has been associated with increased anterior surface inflammatory processes that include dry eye syndromes and ocular allergy. In addition, poorer outcomes of LASIK procedures have been reported in patients with moderate to severe ocular allergies and chronic forms of allergic conjunctivitis, which is an absolute contraindication to the LASIK procedure. We propose preoperative and postoperative management algorithms for allergic LASIK candidates.

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Interleukin-6 in Tear Fluid After Photorefractive Keratectomy and Its Effects on Keratocytes in Culture

Cited by 39 publications

References 0 publications

Enhanced Release of IL-6 and IL-8 into Tears in Various Anterior Segment Eye Diseases

Enhanced Release of IL-6 and IL-8 into Tears in Various Anterior Segment Eye Diseases

Wound healing following refractive surgery in hens

Allergic complications with laser-assisted in-situ keratomileusis

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