Interleukin-6 (IL-6) production by cytokine-stimulated human Müller cells

Yoshida, Shuhei; Sotozono, Chie; Ikeda, Tsunehiko; Kinoshita, Shigeru

doi:10.1076/ceyr.22.5.341.5498

Cited by 42 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the retina, IL-6 is synthesized by Müller cells and the RPE (Benson et al, 1992;Yoshida et al, 2001). A neuroprotective role for IL-6 has been suggested in different animal models of ocular injury, in vitro studies, retinal vein occlusion, diabetic macular oedema and experimental glaucoma (Chong et al, 2008;Funatsu et al, 2009;Noma et al, 2009) suggesting that IL-6 upregulation after injury may serve to control photoreceptor apoptosis (Chong et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Phosphodiesterase inhibition induces retinal degeneration, oxidative stress and inflammation in cone-enriched cultures of porcine retina

Cámara

Sequedo

Gómez‐Pinedo

et al. 2013

Experimental Eye Research

View full text Add to dashboard Cite

Inherited retinal degenerations affecting both rod and cone photoreceptors constitute one of the causes of incurable blindness in the developed world. Cyclic guanosine monophosphate (cGMP) is crucial in the phototransduction and, mutations in genes related to its metabolism are responsible for different retinal dystrophies. cGMP-degrading phosphodiesterase 6 (PDE6) mutations cause around 4-5% of the retinitis pigmentosa, a rare form of retinal degeneration. The aim of this study was to evaluate whether pharmacological PDE6 inhibition induced retinal degeneration in cone-enriched cultures of porcine retina similar to that found in murine models. PDE6 inhibition was induced in cone-enriched retinal explants from pigs by Zaprinast. PDE6 inhibition induced cGMP accumulation and triggered retinal degeneration, as determined by TUNEL assay. Western blot analysis and immunostaining indicated that degeneration was accompanied by caspase-3, calpain-2 activation and poly (ADP-ribose) accumulation. Oxidative stress markers, total antioxidant capacity, thiobarbituric acid reactive substances (TBARS) and nitric oxide measurements revealed the presence of oxidative damage. Elevated TNF-alpha and IL-6, as determined by enzyme immunoassay, were also found in cone-enriched retinal explants treated with Zaprinast. Our study suggests that this ex vivo model of retinal degeneration in porcine retina could be an alternative model for therapeutic research into the mechanisms of photoreceptor death in cone-related diseases, thus replacing or reducing animal experiments.

show abstract

Section: Discussionmentioning

confidence: 99%

Phosphodiesterase inhibition induces retinal degeneration, oxidative stress and inflammation in cone-enriched cultures of porcine retina

Cámara

Sequedo

Gómez‐Pinedo

et al. 2013

Experimental Eye Research

View full text Add to dashboard Cite

show abstract

“…For example, IL-1b (interleukin-1b) and TNF-a are potent inducers of IL-8 expression in Müller cells (Liu et al, 2014b), and IL1b and lipopolysaccharide (LPS) also provoke IL-6 production (Yoshida et al, 2001). IL-6 is a key cytokine in the host's defence against environmental stress, such as infection, inflammation and injury (Mesquida et al, 2014).…”

Section: Immune Responsesmentioning

confidence: 99%

Glia–neuron interactions in the mammalian retina

Vecino

Rodríguez

Ruzafa

et al. 2016

Progress in Retinal and Eye Research

615

533

View full text Add to dashboard Cite

The mammalian retina provides an excellent opportunity to study glia-neuron interactions and the interactions of glia with blood vessels. Three main types of glial cells are found in the mammalian retina that serve to maintain retinal homeostasis: astrocytes, Müller cells and resident microglia. Müller cells, astrocytes and microglia not only provide structural support but they are also involved in metabolism, the phagocytosis of neuronal debris, the release of certain transmitters and trophic factors and K(+) uptake. Astrocytes are mostly located in the nerve fibre layer and they accompany the blood vessels in the inner nuclear layer. Indeed, like Müller cells, astrocytic processes cover the blood vessels forming the retinal blood barrier and they fulfil a significant role in ion homeostasis. Among other activities, microglia can be stimulated to fulfil a macrophage function, as well as to interact with other glial cells and neurons by secreting growth factors. This review summarizes the main functional relationships between retinal glial cells and neurons, presenting a general picture of the retina recently modified based on experimental observations. The preferential involvement of the distinct glia cells in terms of the activity in the retina is discussed, for example, while Müller cells may serve as progenitors of retinal neurons, astrocytes and microglia are responsible for synaptic pruning. Since different types of glia participate together in certain activities in the retina, it is imperative to explore the order of redundancy and to explore the heterogeneity among these cells. Recent studies revealed the association of glia cell heterogeneity with specific functions. Finally, the neuroprotective effects of glia on photoreceptors and ganglion cells under normal and adverse conditions will also be explored.

show abstract

“…Müller cells produce IL-6 [202,203], e.g. upon stimulation with IL-1ß and advanced glycation end products [204,205]. Possibly, Müller cells secrete IL-6 as a factor that protects retinal ganglion cells and photoreceptors from cell death [206][207][208][209].…”

Section: Factors Involved In Müller Cell Gliosis and Proliferationmentioning

confidence: 99%

Involvement of Müller glial cells in epiretinal membrane formation

Bringmann

Wiedemann

2009

Graefes Arch Clin Exp Ophthalmol

173

114

View full text Add to dashboard Cite

show abstract

Interleukin-6 (IL-6) production by cytokine-stimulated human Müller cells

Abstract: Our findings indicate that human Müller cells can produce IL-6 when stimulated by IL-1beta or LPS. Müller cell IL-6 production may have an important role in various conditions involving ocular inflammation

Cited by 42 publications

References 19 publications

Phosphodiesterase inhibition induces retinal degeneration, oxidative stress and inflammation in cone-enriched cultures of porcine retina

Phosphodiesterase inhibition induces retinal degeneration, oxidative stress and inflammation in cone-enriched cultures of porcine retina

Glia–neuron interactions in the mammalian retina

Involvement of Müller glial cells in epiretinal membrane formation

Contact Info

Product

Resources

About