Interleukin-6 bedside test in detecting chorioamnionitis in women with preterm premature rupture of fetal membranes

Abstract

Objectives: About 30-80% of preterm deliveries following preterm premature rupture of fetal membranes (PPROM) are complicated by histological chorioamnionitis. To evaluate the accuracy of Interleukin-6 (IL-6) bedside test in detecting chorioamnionitis in women with PPROM. Material and methods: One hundred and ten (110) pregnant women with PPROM > 24 and < 34 weeks' gestation, admitted for conservative management were included in this study. Participants were examined on admission using sterile speculum examination for assessment of IL-6 in the cervico-vaginal secretions using the IL-6 bedside test. The IL-6 bedside test was repeated for all participants once termination of pregnancy (TOP) decided. After TOP, placenta, umbilical cord, and fetal membranes samples were examined for histologic detection of chorioamnionitis (gold standard). The histological results were compared with IL-6 bedside test results to evaluate the accuracy of IL-6 bedside test in detecting chorioamnionitis in women with PPROM. Results:The IL-6 bedside test had 98.6% sensitivity, 94.7% specificity, 97.3% positive predictive value (PPV), 97.3% negative predictive value (NPV) and 97.3% overall accuracy in detecting chorioamnionitis. The sensitivity, specificity, NPV and overall accuracy of IL-6 bedside test (98.6%, 94.7%, 97.3%, and 97.3%; respectively) were significantly higher than the clinical and laboratory parameters of chorioamnionitis (65.3%, 57.9%, 46.8%, and 62.7%; respectively) (p = 0.04, 0.02, 0.001 and 0.03; respectively). Conclusions:The IL-6 bedside test is an accurate, non-invasive bedside test with 98.6% sensitivity, 94.7% specificity, 97.3% PPV, 97.3% NPV, and 97.3% overall accuracy in detecting chorioamnionitis. The IL-6 bedside test had significantly higher sensitivity, specificity, NPV, and overall accuracy than the clinical and laboratory parameters of chorioamnionitis.

Evaluating the IL‐6 Family of Cytokines Throughout Equine Gestation

“…The additional members of this family (IL-27, IL-35, and IL-39) were identified more recently as being heterodimeric cytokines with a common subunit encoded by Epstein-Barr virus-induced gene 3 (EB13) [17]. Currently, elevated IL-6 is utilized as a biomarker for pregnancy dysfunction, and has been implicated or associated with chorioamnionitis [8,11,18,19], preeclampsia [20][21][22], and premature rupture of membranes [7,[23][24][25], and this elevation in IL-6 has been noted during placental disease in the horse [5,12,[26][27][28]. Little research has been done to assess IL-6 or its associated family members during healthy pregnancy.…”