Interleukin-5 Receptor α Subunit Gene Regulation in Human Eosinophil Development: Identification of a Unique Cis-Element that acts lie an Enhacer in Regulating Activity of the IL-5Rα Promoter

An AP‐1 site in the promoter of the human IL‐5Rα gene is necessary for promoter activity in eosinophilic HL60 cells

“…The promoter region contains multiple consensus sites for known hematopoietic transcription factors, including GATA, PU1, C/EBP, and AP‐1. Sun et al [12]identified a unique enhancer‐like cis element (EOS1) that is necessary and sufficient for promoter activity and binds an unknown transcription factor. In this study we have studied the involvement of an AP‐1 site in the regulation of the IL‐5Rα gene.…”

Cloning of the Rat IL-5Rα Gene: Analysis of 5′-Upstream Region and Expression by B Cells

“…This 6-bp element has been described in the P2 promoter of human IL-5R␣ and has been shown to be required for promoting the IL-5R␣ gene transcription in an eosinophilic manner (49). However, we did not find an EOS-1 site that is necessary and sufficient for human IL-5R␣ P1 promoter activity (55).…”

Engagement of the CrkL Adapter in Interleukin-5 Signaling in Eosinophils

“…Preparation of Whole Cell and Nuclear Extracts-Nuclear extracts were prepared using a rapid micropreparation technique for small numbers of cells (58) with minor modifications, including the use of protease inhibitor mixture tablets (Roche Molecular Biochemicals, Mannheim, Germany) and the addition of phenylmethylsulfonyl fluoride (0.5 mM) and diisopropyl fluorophosphate (1 mM) to the resuspension and lysis buffers (59,60).…”