Interleukin-4 Promotes Tuft Cell Differentiation and Acetylcholine Production in Intestinal Organoids of Non-Human Primate

Inaba, Akihiko; Arinaga, Ayane; Tanaka, Keisuke; Endo, Takaho A.; Hayatsu, Norihito; Okazaki, Yasushi; Yamane, Takumi; Oishi, Yoshifumi; Imai, Hiroo; Iwatsuki, Ken

doi:10.3390/ijms22157921

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…Uniquely, these spheroids represent all major epithelial cell lineages within the organoid (Sato et al, 2011), and allow to skew them towards specific cell types and rare lineages like tuft cells (Gerbe et al, 2016;Treveil et al, 2020;Inaba et al, 2021). Even the most sophisticated non-stem cell models still fail to replicate the lineage complexity to this extent (Lozoya-Agullo et al, 2017;Lehner et al, 2020;Kämpfer et al, 2022).…”

Section: Intestinal Modelsmentioning

confidence: 99%

Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

et al. 2023

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Plastic particles in the nanometer range–called nanoplastics–are environmental contaminants with growing public health concern. As plastic particles are present in water, soil, air and food, human exposure via intestine and lung is unavoidable, but possible health effects are still to be elucidated. To better understand the Mode of Action of plastic particles, it is key to use experimental models that best reflect human physiology. Novel assessment methods like advanced cell models and several alternative approaches are currently used and developed in the scientific community. So far, the use of cancer cell line-based models is the standard approach regarding in vitro nanotoxicology. However, among the many advantages of the use of cancer cell lines, there are also disadvantages that might favor other approaches. In this review, we compare cell line-based models with stem cell-based in vitro models of the human intestine and lung. In the context of nanoplastics research, we highlight the advantages that come with the use of stem cells. Further, the specific challenges of testing nanoplastics in vitro are discussed. Although the use of stem cell-based models can be demanding, we conclude that, depending on the research question, stem cells in combination with advanced exposure strategies might be a more suitable approach than cancer cell lines when it comes to toxicological investigation of nanoplastics.

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Section: Intestinal Modelsmentioning

confidence: 99%

Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

et al. 2023

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“…However, IL-25 production of human tuft cells has only been described in the nasal epithelium [ 84 – 86 ]. IL-25 transcripts are not robustly detected in tuft cells from intestinal tissue in nonhuman primates or humans [ 22 , 42 ]. Whether human tuft cells produce an alternative effector molecule instead of IL-25 and under what circumstances human tuft cells promote type 2 immunity is an active area of investigation.…”

Section: Tuft Cell Heterogeneitymentioning

confidence: 99%

Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces

Strine

Wilen

2022

PLoS Pathog

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Although tuft cells were discovered over 60 years ago, their functions have long been enigmatic, especially in human health. Nonetheless, tuft cells have recently emerged as key orchestrators of the host response to diverse microbial infections in the gut and airway. While tuft cells are epithelial in origin, they exhibit functions akin to immune cells and mediate important interkingdom interactions between the host and helminths, protists, viruses, and bacteria. With broad intra- and intertissue heterogeneity, tuft cells sense and respond to microbes with exquisite specificity. Tuft cells can recognize helminth and protist infection, driving a type 2 immune response to promote parasite expulsion. Tuft cells also serve as the primary physiologic target of persistent murine norovirus (MNV) and promote immune evasion. Recently, tuft cells were also shown to be infected by rotavirus. Other viral infections, such as influenza A virus, can induce tuft cell–dependent tissue repair. In the context of coinfection, tuft cells promote neurotropic flavivirus replication by dampening antiviral adaptive immune responses. Commensal and pathogenic bacteria can regulate tuft cell abundance and function and, in turn, tuft cells are implicated in modulating bacterial infiltration and mucosal barrier integrity. However, the contribution of tuft cells to microbial sensing in humans and their resulting effector responses are poorly characterized. Herein, we aim to provide a comprehensive overview of microbial activation of tuft cells with an emphasis on tuft cell heterogeneity and differences between mouse and human tuft cell biology as it pertains to human health and disease.

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“…Refs. 23,24 ), adding other cell types to the 3D cultures (e.g. co-culture with immune cell types or the generation of spheroids or OOAC systems with containing more than one cell type), or in the case of inter-organ communication, connecting different types of organoid or other multicellular model within the same culture to generate a more model system with greater complexity and which more closely mimics in vivo biology.…”

Section: Benefits and Limitations Of 3d Cell Culture Systemsmentioning

confidence: 99%

“…It is therefore important to recognise these limitations, for example by thorough characterisation of the cell types present in the 3D culture and comparing this with the cell types expected in vivo, and to address these limitations where possible, for example by defining media formulations which allow terminal differentiation of desired cell types (e.g. Refs 23,24 …”

Section: Introductionmentioning

confidence: 99%

Advancing animal health and disease research in the lab with three‐dimensional cell culture systems

et al. 2022

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The development of three‐dimensional cell culture systems representative of tissues from animals of veterinary interest is accelerating research that seeks to address specific questions tied to animal health. In terms of their relevance and complexity, these in vitro models can be seen as a midpoint between the more reductionist single‐cell culture systems and complex live animals. Organoids in particular represent a significant development due to their organised multicellular structure that more closely represents in vivo tissues than any other cell culture technology previously developed. In this review, we provide an overview of the different three‐dimensional cell culture systems available to veterinary researchers and give examples of their application in contexts relating to animal health.

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Interleukin-4 Promotes Tuft Cell Differentiation and Acetylcholine Production in Intestinal Organoids of Non-Human Primate

Cited by 9 publications

References 35 publications

Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

Investigating nanoplastics toxicity using advanced stem cell-based intestinal and lung in vitro models

Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces

Advancing animal health and disease research in the lab with three‐dimensional cell culture systems

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