Interleukin-4, Interleukin-8 and E-selectin Levels in Intranasal Polyposis Patients with and without Allergy: A Comparative Study

Ural, Ahmet; Tezer,; Yücel, Ayşegül Atak; Atilla, Huntürk; Ileri, Fikret

doi:10.1177/147323000603400509

Cited by 15 publications

(17 citation statements)

References 13 publications

(19 reference statements)

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“…IL-6 has a chemotactic effect on eosinophils and IL-8 affects both neutrophils and eosinophils, causing their migration to the site of inflammation 15. According to recent studies, increased levels of IL-6 and IL-8 may participate in the primary pathogenesis of CRS and NP as well as in the recurrent episodes 16,17,18. IL-6 is a multifunctional cytokine implicated in various inflammatory conditions including NP pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin E2 Induces IL-6 and IL-8 Production by the EP Receptors/Akt/NF-κB Pathways in Nasal Polyp-Derived Fibroblasts

Cho

Han

Lee

et al. 2014

Allergy Asthma Immunol Res

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PurposeInterleukin 6 (IL-6) and IL-8 participate in the pathogenesis of chronic rhinosinusitis with nasal polyps, and their levels are increased by prostaglandin E2 (PGE2) in different cell types. The purposes of this study were to determine whether PGE2 has any effect on the increase in the levels of IL-6 and IL-8 in nasal polyp-derived fibroblasts (NPDFs) and subsequently investigate the possible mechanism of this effect.MethodsDifferent concentrations of PGE2 were used to stimulate NPDFs at different time intervals. NPDFs were treated with agonists and antagonists of E prostanoid (EP) receptors. To determine the signaling pathway for the expression of PGE2-induced IL-6 and IL-8, PGE2 was treated with Akt and NF-κB inhibitors in NPDFs. Reverse transcription-polymerase chain reaction for IL-6 and IL-8 mRNAs was performed. IL-6 and IL-8 levels were measured byenzyme-linked immunosorbent assay (ELISA). The activation of Akt and NF-κB was evaluated by western blot analysis.ResultsPGE2 significantly increased the mRNA and protein expression levels of IL-6 and IL-8 in NPDFs. The EP2 and EP4 agonists and antagonists induced and inhibited IL-6 expression. However, the EP4 agonist and antagonist were only observed to induce and inhibit IL-8 expression level. The Akt and NF-κB inhibitors significantly blocked PGE2-induced expression of IL-6 and IL-8.ConclusionsPGE2 increases IL-6 expression via EP2 and EP4 receptors, and IL-8 expression via the EP4 receptor in NPDFs. It also activates the Akt and NF-κB signal pathways for the production of IL-6 and IL-8 in NPDFs. These results suggest that signaling pathway for IL-6 and IL-8 expression induced by PGE2 might be a useful therapeutic target for the treatment of nasal polyposis.

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Section: Discussionmentioning

confidence: 99%

Prostaglandin E2 Induces IL-6 and IL-8 Production by the EP Receptors/Akt/NF-κB Pathways in Nasal Polyp-Derived Fibroblasts

Cho

Han

Lee

et al. 2014

Allergy Asthma Immunol Res

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“…The chemokine IL-8 has been detected in nasal secretions of patients with AR upon allergen challenge [13] and its concentration showed a significant correlation with the symptom score [14]. Furthermore, IL-8 has been shown to be up-regulated in nasal polyps of AR patients and in the nasal mucosa of patients with polyposis as well as in nasal sinusitis [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Dissolution in nasal fluid, retention and anti‐inflammatory activity of fluticasone furoate in human nasal tissue ex vivo

Baumann

Bachert

Högger

2009

Clin Experimental Allergy

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“…In fact we found an irregular and low expression of E-selectin in nasal polyps. Ural et al [34] concluded that it is not entirely justifiable that E-selectin has a role in the pathogenesis of intranasal polyposis.…”

Section: Discussionmentioning

confidence: 99%

Immune Imbalance in Nasal Polyps of Caucasian Chronic Rhinosinusitis Patients Is Associated with a Downregulation of E-Selectin

Könnecke

Böscke

Waldmann

et al. 2014

Journal of Immunology Research

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Chronic rhinosinusitis with nasal polyps (CRSwNP) in Caucasians is a chronic Th2 inflammatory disease of the nasal and paranasal mucosa and the recruitment of leukocytes to the site of inflammation is poorly understood. We studied mRNA and protein expression profiles of adhesion molecules in nasal polyp and associated inferior turbinate tissues using molecular, biochemical, and immunohistological methods. Analysis showed a strongly decreased E-selectin expression in nasal polyps with a significant difference between eosinophil and neutrophil counts in nasal polyps and balanced counts in inferior turbinates. E-selectin expression is known to be downregulated in a Th2 milieu and has an essential role in immunosurveillance by locally activating neutrophil arrest and migratory function. A downregulation of E-selectin may come along with an immune imbalance in Caucasian nasal polyps due to a significant inhibition of neutrophil recruitment. Therefore, we suggest that an upregulation of E-selectin and the associated influx of neutrophils may play a significant role in the resolution of inflammation as well as for the pathophysiology of nasal polyps of Caucasian chronic rhinosinusitis patients.

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Interleukin-4, Interleukin-8 and E-selectin Levels in Intranasal Polyposis Patients with and without Allergy: A Comparative Study

Cited by 15 publications

References 13 publications

Prostaglandin E2 Induces IL-6 and IL-8 Production by the EP Receptors/Akt/NF-κB Pathways in Nasal Polyp-Derived Fibroblasts

Prostaglandin E2 Induces IL-6 and IL-8 Production by the EP Receptors/Akt/NF-κB Pathways in Nasal Polyp-Derived Fibroblasts

Dissolution in nasal fluid, retention and anti‐inflammatory activity of fluticasone furoate in human nasal tissue ex vivo

Immune Imbalance in Nasal Polyps of Caucasian Chronic Rhinosinusitis Patients Is Associated with a Downregulation of E-Selectin

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