Interleukin-35 Mitigates ox-LDL-Induced Proatherogenic Effects via Modulating miRNAs Associated with Coronary Artery Disease (CAD)

Bhansali, Shipra; Yadav, Amit K.; Bakshi, Chetan; Dhawan, V.

doi:10.1007/s10557-022-07335-x

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…The data indicated that hsa-miR-197–5p was significantly up-regulated in both the CAD/healthy and MI/healthy groups. Previous studies have demonstrated that hsa-miR-197–5p may play a crucial role in controlling the anti-inflammatory response of IL-35 by influencing the secretion of cytokines that can either promote or suppress inflammation, the ratio of M1/M2 macrophages, and the proliferation of T lymphocytes, which are responsible for suppressing immune responses [ 44 ]. Alongside our findings, it can be concluded that hsa-miR-197–5p could be a useful diagnostic tool for predicting adverse cardiovascular events.…”

Section: Discussionmentioning

confidence: 99%

Unlocking the potential of microRNAs: machine learning identifies key biomarkers for myocardial infarction diagnosis

Samadishadlou,

Rahbarghazi,

Piryaei

et al. 2023

Cardiovasc Diabetol

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Background MicroRNAs (miRNAs) play a crucial role in regulating adaptive and maladaptive responses in cardiovascular diseases, making them attractive targets for potential biomarkers. However, their potential as novel biomarkers for diagnosing cardiovascular diseases requires systematic evaluation. Methods In this study, we aimed to identify a key set of miRNA biomarkers using integrated bioinformatics and machine learning analysis. We combined and analyzed three gene expression datasets from the Gene Expression Omnibus (GEO) database, which contains peripheral blood mononuclear cell (PBMC) samples from individuals with myocardial infarction (MI), stable coronary artery disease (CAD), and healthy individuals. Additionally, we selected a set of miRNAs based on their area under the receiver operating characteristic curve (AUC-ROC) for separating the CAD and MI samples. We designed a two-layer architecture for sample classification, in which the first layer isolates healthy samples from unhealthy samples, and the second layer classifies stable CAD and MI samples. We trained different machine learning models using both biomarker sets and evaluated their performance on a test set. Results We identified hsa-miR-21-3p, hsa-miR-186-5p, and hsa-miR-32-3p as the differentially expressed miRNAs, and a set including hsa-miR-186-5p, hsa-miR-21-3p, hsa-miR-197-5p, hsa-miR-29a-5p, and hsa-miR-296-5p as the optimum set of miRNAs selected by their AUC-ROC. Both biomarker sets could distinguish healthy from not-healthy samples with complete accuracy. The best performance for the classification of CAD and MI was achieved with an SVM model trained using the biomarker set selected by AUC-ROC, with an AUC-ROC of 0.96 and an accuracy of 0.94 on the test data. Conclusions Our study demonstrated that miRNA signatures derived from PBMCs could serve as valuable novel biomarkers for cardiovascular diseases.

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Section: Discussionmentioning

confidence: 99%