Interleukin-3 promotes neurite outgrowth and elevates choline acetyltransferase activity in vitro.

Kamegai, Masahiro; Kunishita, Tatsuhide; Tokuchi, Fumio; Nishizawa, Manabu; Tabira, Takeshi

doi:10.2183/pjab.65.17

Cited by 4 publications

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“…In our previous studies, we demonstrated that IL‐3 has a neurite‐promoting effect on primary cultured neurons derived from the septal region and elevates choline acetyltransferase (ChAT) activity in these neurons as well as in a central cholinergic hybrid neuronal cell line SN6. 1,2 In addition to the in vitro effect, intraventricularly injected IL‐3 promoted neuronal survival in vivo in fimbria‐fornix (F‐F)‐transected rats. 2 Because most of the cytokines do not seem to cross the blood‐brain barrier, we looked for the source of IL‐3 in the brain, and IL‐3 mRNA was detected in primary cultured hippocampal neurons (but not in septal neurons) and in mixed glia cells by reverse transcription and polymerase chain reaction (RTPCR).…”

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Interleukin‐3 and Interleukin‐3 Receptors in the Brain^a

Tabira

Chui

Fan

et al. 1998

Annals of the New York Academy of Sciences

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We have previously demonstrated that interleukin 3 (IL-3) has a neurotrophic effect on central cholinergic neurons and have demonstrated the presence of IL-3 receptor (IL-3R)beta subunits in septal cholinergic neurons by reverse-transcribed polymerase chain reaction (RT-PCR) and immunohistochemistry. In order to confirm that the expressed IL-3R is functional, we conducted experiments to show an alpha subunit of IL-3R. The alpha subunit was clearly demonstrated by RT-PCR in the central cholinergic neuronal hybrid cell line SN6, but not in its mother cell line N18TG2, and the expression was slightly upregulated after IL-3 treatment. Choline acetyltransferase and vesicular acetylcholine transporter mRNAs were significantly increased in SN6 after treatment with IL-3. Immunohistochemically, IL-3R alpha-positive cells were mainly present in the medial septal and basal forebrain region, and the stained cells were similar to choline acetyltransferase-positive cells in shape and distribution. The IL-3R alpha-positive cells slightly increased two days after fimbria-fornix transection and decreased seven days after. These findings suggest that functional IL-3 receptors are expressed in the central cholinergic neurons and contribute to some physiological roles such as the differentiation and maintenance of these neurons.

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confidence: 99%