Interleukin‐28B polymorphisms and interferon gamma inducible protein‐10 serum levels in seronegative occult hepatitis C virus infection

Bartolomé, Javier; Castillo, Inmaculada; Quiroga, Juan Antonio; Carréño, Vicente

doi:10.1002/jmv.24322

Cited by 9 publications

(12 citation statements)

References 36 publications

(52 reference statements)

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“…One probable explanation is that HCV has developed a number of evasion mechanisms, infection of PBMCs being one of those where the virus can avoid the immune defense system, while hepatocytes remain the actual target. Previous works demonstrated that patients with seronegative HCV have an HCV-specific cellular immune response with a probable immune surveillance function, suggesting that host immune response is able to control but not to eliminate, HCV replication in these cases (27). It may also be that the virus developed some new quasi-species in PBMC that showed delayed response to the antiviral therapy but we could not confirm that speculation now.…”

Section: Discussioncontrasting

confidence: 57%

The Role of Polymorphisms Near IFNL3 Gene as Predictors of Residual HCV RNA in Buffy Coat after Successful Antiviral Therapy

et al. 2017

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Background and Aims: The presence of the hepatitis C virus (HCV) in cells of extrahepatic organs like peripheral blood mononuclear cells (PBMCs) have important implications for transmission, disease progression, and effective treatment of HCV-infected patients. The impact of host genetics such as polymorphisms near Interferon lambda 3 (IFNL3) on clearance of HCV RNA from buffy coat (BC) following successful clearance of HCV from plasma using Pegylated-IFN (PegIFN) and Ribavirin (RBV) treatment was evaluated in our study. Methods: For detection of residual HCV RNA in BC samples, blood samples of 69 patients with sustained virologic response (SVR) after treatment with PegIFN and RBV were evaluated. Polymorphisms near IFNL3 gene including rs12979860 and rs8099917 were assessed using PCR-RFLP method. Results: The most prevalent rs12979860 and rs8099917 genotypes were CT (49.3%) and TT (62.3%), respectively. Nine (13.04%, 95%CI: 7.01% -22.96%) patients had HCV RNA in their BC samples. The favorable genotypes of the 2 polymorphisms (rs12979860 CC and rs8099917 TT) were more frequently observed in patients with undetectable HCV RNA in their BC samples than those with HCV RNA in their BC samples (rs12979860 CC, 45% vs. 22.2%, P = 0.016 and rs8099917 TT, 66.7% vs. 33.3%, P = 0.01). Conclusions:The polymorphisms of IFNL3 could play a crucial role not only in spontaneous clearance of HCV and SVR rate after PegIFN and RBV therapy, but also in the clearance of HCV from BC after PegIFN and RBV therapy.

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Section: Discussioncontrasting

confidence: 57%

The Role of Polymorphisms Near IFNL3 Gene as Predictors of Residual HCV RNA in Buffy Coat after Successful Antiviral Therapy

et al. 2017

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“…In contrast, Bartolomé et al . indicated that favorable IFNL3 rs12979860 was the most dominant genotype in patients with OCI . The reason why IFNL3 rs12979860 genotype is associated with OCI is unclear and its explanation requires further studies.…”

Section: Discussionmentioning

confidence: 97%

“…38 In contrast, Bartolomé et al indicated that favorable IFNL3 rs12979860 was the most dominant genotype in patients with OCI. 39 The reason why IFNL3 rs12979860 genotype is associated with OCI is unclear and its explanation requires further studies. However, several studies showed that IFNL3 rs12979860 non-CC genotypes related to higher ISG expression levels in the setting of CHC infection were previously correlated with lower levels of spontaneous clearance, and non-responding patients had significantly higher ISG expression compared with subjects that achieved SVR after treatment.…”

Section: T He Current Study Comprehensively Evaluated the Prevalence mentioning

confidence: 99%

Host genetic factors and clinical parameters influencing the occult hepatitis C virus infection in patients on chronic hemodialysis: Is it still a controversial infection?

Ayadi

Nafari

Sakhaee

et al. 2019

Hepatology Research

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Aim The presence of occult hepatitis C virus (HCV) infection (OCI) is still controversial, however, this infection cannot be ignored. Therefore, the current study aimed at assessing the OCI frequency in patients on chronic hemodialysis (CHD) and also evaluating the association between OCI incidence with clinical parameters and interferon lambda 3/4 (IFNL3/4) gene polymorphisms. Methods A total of 515 patients on CHD and HCV negative markers were selected. Plus‐ and minus‐stranded HCV‐RNA was tested in peripheral blood mononuclear cell samples by reverse transcription–polymerase chain reaction and then genotyped using the restriction fragment length polymorphism method. Results The frequency of OCI was 11.3% in patients on CHD. Among 58 patients with OCI, 25.8%, 62.1%, and 12.1% were infected with HCV‐1a, HCV‐1b, and HCV‐3a, respectively. The mean alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase levels were 31.9 ± 24.8, 32.3 ± 19.1, and 171.6 ± 88.9, respectively. None of the patients with OCI had a history of liver disease. Multivariate logistic regression analysis indicated that cholesterol, triglyceride, low‐density lipoprotein, 25‐hydroxyvitamin D, platelets, duration of hemodialysis, HCV subtypes, IFNL3 rs12979860 TT, IFNL3 rs8099917 GG, IFNL3 rs12980275 GG, and IFNL4 ss469415590 ΔG/ΔG genotypes were associated with OCI. Conclusion There was a moderate prevalence of OCI in Iranian patients on CHD. The current study findings indicated that this infection was associated with clinical parameters and unfavorable genotypes of IFNL3 single nucleotide polymorphisms and IFNL4 ss469415590. Further studies are required to determine the correlation between OCI incidence with clinical parameters and host genetic factors.

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“…Patients with OCI have distinct clinical outcomes from those with CHC even of the same genotypes, indicating the role of host factors in OCI pathogenesis. The interleukin-28B (IL-28B) locus has been associated with HCV outcomes and IL-28B C/C was shown to occur more often in OCI than in CHC patients[ 19 ]. In OCI patients, intrahepatic HCV RNA load was significantly lower in those with the IL-28B C/C genotype than in those with C/T or T/T genotypes[ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…The interleukin-28B (IL-28B) locus has been associated with HCV outcomes and IL-28B C/C was shown to occur more often in OCI than in CHC patients[ 19 ]. In OCI patients, intrahepatic HCV RNA load was significantly lower in those with the IL-28B C/C genotype than in those with C/T or T/T genotypes[ 19 ]. Thus, IL-28B polymorphisms may affect endogenous IFN-λ levels and be associated with low viral replication in some patients.…”

Section: Introductionmentioning

confidence: 99%

Regulatory polymorphism of CXCL10 rs1439490 in seronegative occult hepatitis C virus infection

Wang

Liu

et al. 2018

WJG

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AIMTo examine the relationship between the single nucleotide polymorphism CXCL10 rs1439490 and seronegative occult hepatitis C virus (HCV) infection (OCI).METHODSOne hundred and three cases of seronegative OCI and 155 cases of seropositive chronic HCV infection (CHC) were diagnosed at five Liver Centers in Northeastern China, from 2012 to 2016. CXCL10 rs1439490, rs1440802, and IL-28B rs12979860 were analyzed by sequencing. Serum CXCL10 was measured by ELISA. Intrahepatic CXCL10 was determined by quantitative PCR and immunohistochemical semi-quantitative scoring. Liver necroinflammation and fibrosis were scored according to the METAVIR system.RESULTSCXCL10 rs1439490 G/G was more prevalent in OCI patients (n = 93/103; 90.3%) than in CHC patients (n = 116/155; 74.8%; P = 0.008). OCI patients had lower serum CXCL10 levels than CHC patients (192.91 ± 46.50 pg/mL vs 354.78 ± 102.91 pg/mL, P < 0.0001). Of IL-28B rs12979860 C/C patients, OCI patients with rs1439490 G/G had lower serum and liver levels of CXCL10 and lower levels of liver necroinflammation and fibrosis than non-G/G patients. OCI patients had higher alanine aminotransferase normalization rates after Peg-interferon treatment than CHC patients (P < 0.05) and serum CXCL10 decreased significantly (P < 0.0001). Liver necroinflammation and fibrosis were alleviated in 8 OCI patients after treatment. Multivariate analysis indicated that rs1439490 G/G significantly influenced the occurrence of OCI in HCV infection (OR = 0.31, 95%CI: 0.15-0.66, P = 0.002).CONCLUSIONCXCL10 rs1439490 G/G is positively associated with OCI in HCV infection and antiviral outcome.

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Interleukin‐28B polymorphisms and interferon gamma inducible protein‐10 serum levels in seronegative occult hepatitis C virus infection

Cited by 9 publications

References 36 publications

The Role of Polymorphisms Near IFNL3 Gene as Predictors of Residual HCV RNA in Buffy Coat after Successful Antiviral Therapy

The Role of Polymorphisms Near IFNL3 Gene as Predictors of Residual HCV RNA in Buffy Coat after Successful Antiviral Therapy

Host genetic factors and clinical parameters influencing the occult hepatitis C virus infection in patients on chronic hemodialysis: Is it still a controversial infection?

Regulatory polymorphism of CXCL10 rs1439490 in seronegative occult hepatitis C virus infection

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