Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47phox

Sowrirajan, Bharatwaj; Saito, Yoshiro; Poudyal, Deepak; Chen, Qian; Sui, Hongyan; Ravin, Suk See De; Imamichi, Hiromi; Sato, Toyotaka; Kuhns, Douglas B.; Noguchi, Noriko; Malech, Harry L.; Lane, H. Clifford; Imamichi, Tomozumi

doi:10.1038/srep43441

Cited by 20 publications

(19 citation statements)

References 53 publications

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“…S8 ). Given that, miR-SX4 was first detected in IL-27 treated macrophages 31 and we have previously reported that IL-27 exerts anti-HIV effects in Monocyte Derived Macrophages (MDM) 29 , 30 , 66 , CD4+T cells 67 and mature DCs 68 . Therefore, there is also a potential that miR-SX4 may functionally exert anti-HIV effects in these primary target cells of HIV virus.…”

Section: Discussionmentioning

confidence: 99%

“…The protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific). Using a total of 20 μg protein, western blot analysis was performed as previously described 66 . Antibody binding was visualized using ECL Prime Western Detection Reagent (GE-Healthcare) and LAS-4000 (Fujifilm, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

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A novel microRNA, hsa-miR-6852 differentially regulated by Interleukin-27 induces necrosis in cervical cancer cells by downregulating the FoxM1 expression

Poudyal

Herman

Adelsberger

et al. 2018

Sci Rep

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We have previously demonstrated that Interleukin-27 differentially regulates the expression of seven novel microRNAs. Here we elucidate the functional significance of these novel microRNAs. Of the seven microRNAs, over expression of miRNA-6852 (miR-SX4) mimic induces cell cycle arrest at G2/M phase and induces necrosis in HEK293 and panel of cervical cancer cells (Human Papilloma Virus (HPV) infected cell lines; HeLa, CaSki and SiHa cells). To define the mechanism of the miR-SX4-mediated G2/M arrest, a microarray gene chip array and western blot analysis were performed. FoxM1, a transcription factor is identified as a key protein down-regulated by miR-SX4, even though the miR-SX4 does not target 3’UTR of FoxM1. Knock down of FoxM1 using si-RNA demonstrate that FoxM1 silenced cell induces G2/M cell cycle arrest and necrosis. Our data demonstrated for the first time that miR-SX4 could be a potent anti-cancer microRNA.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A novel microRNA, hsa-miR-6852 differentially regulated by Interleukin-27 induces necrosis in cervical cancer cells by downregulating the FoxM1 expression

Poudyal

Herman

Adelsberger

et al. 2018

Sci Rep

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“…Whole-cell lysate proteins (100 μg) were subjected to 2D-PAGE analysis as described [30]. After separation by 2D-PAGE, the samples were transferred to a membrane for western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells

Poudyal¹,

Yang²,

Chen³

et al. 2019

Aids

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Objectives: IL-27 is known as an antiviral cytokine that inhibits HIV, hepatitis C virus, and other viruses. We have previously demonstrated that, IL-27 posttreatment after HIV-infection inhibits viral replication in primary CD4 + T cells. Design: Here, we evaluated the anti-HIV effect of IL-27 pretreatment in CD4 + T cells from healthy donors prior to HIV infection with HIV NL4.3 or vesicular stomatitis virus G glycoprotein (VSV-G)-pseudotyped HIV-luciferase virus (HIV-LUC-V). Methods: IL-27-treated CD4 + T cells were infected with HIV NL4.3 or HIV-LUC-V and assessed the anti-HIV effect. HIV infection was monitored by p24 antigen ELISA or luciferase assay. HIV fusion/entry and uncoating were determined by BlaM-Vpr assay and HIV fate of capsid and/or HIV Entry/Uncoating assay based on core-packaged RNA availability and Translation assay, respectively. HIV proviral copy number was determined by real-time PCR. Gene expression profile from IL-27-pretreated CD4 + T cells was determined using Genechip array. Posttranslational modification of global proteins from IL-27-pretreated CD4 + T cells was determined by a combination of 2-dimensional difference-in-gel-electrophoresis (2D-DIG), western-blot and protein mass spectrometry. Results: IL-27 pretreatment inhibited HIV NL4.3 and HIV-LUC-V infection in CD4 + T cells. HIV copy assay demonstrated that IL-27-treatment suppressed an early step of reverse transcription during HIV infection. A combination of 2D-DIG-electrophoresis and western blot assays demonstrated that IL-27-treatment induces a change in posttranslational modification of Y box binding protein-1 (YB-1). Overexpression of domain negative YB-1 mutants illustrated that a residue Lysine at 118 plays a key role in supporting HIV infection in CD4 + T cells. Conclusion: IL-27-pretreatment inhibits HIV-1 infection by suppressing an HIV-reverse transcription product formation/uncoating step by suppressing the acetylation of YB-1 in primary CD4 + T cells.

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“…Oxidative stress, as a concept formulated in 1985, is induced when ROS generation is increased, or ROS scavengers are dysregulated [ 77 ]. Since oxidative stress is strongly correlated with inflammation and cell death [ 74 , 78 , 79 ], the imbalance of ROS generation and scavenging systems is an alarming signal for cellular metabolisms. In addition, mitochondrial ROS convey information to the nucleus and modulate the expression of nuclear genes accordingly in both physiological [ 80 ] and pathological states [ 66 ].…”

Section: Ros Serve As the New Sensing Network For Homeostasis And Strmentioning

confidence: 99%

ROS systems are a new integrated network for sensing homeostasis and alarming stresses in organelle metabolic processes

et al. 2020

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Reactive oxygen species (ROS) are critical for the progression of cardiovascular diseases, inflammations and tumors. However, the mechanisms of how ROS sense metabolic stress, regulate metabolic pathways and initiate proliferation, inflammation and cell death responses remain poorly characterized. In this analytic review, we concluded that: 1) Based on different features and functions, eleven types of ROS can be classified into seven functional groups: metabolic stress-sensing, chemical connecting, organelle communication, stress branch-out, inflammasome-activating, dual functions and triple functions ROS. 2) Among the ROS generation systems, mitochondria consume the most amount of oxygen; and nine types of ROS are generated; thus, mitochondrial ROS systems serve as the central hub for connecting ROS with inflammasome activation, trained immunity and immunometabolic pathways. 3) Increased nuclear ROS production significantly promotes cell death in comparison to that in other organelles. Nuclear ROS systems serve as a convergent hub and decision-makers to connect unbearable and alarming metabolic stresses to inflammation and cell death. 4) Balanced ROS levels indicate physiological homeostasis of various metabolic processes in subcellular organelles and cytosol, while imbalanced ROS levels present alarms for pathological organelle stresses in metabolic processes. Based on these analyses, we propose a working model that ROS systems are a new integrated network for sensing homeostasis and alarming stress in metabolic processes in various subcellular organelles. Our model provides novel insights on the roles of the ROS systems in bridging metabolic stress to inflammation, cell death and tumorigenesis; and provide novel therapeutic targets for treating those diseases. (Word count: 246).

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Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47phox

Cited by 20 publications

References 53 publications

A novel microRNA, hsa-miR-6852 differentially regulated by Interleukin-27 induces necrosis in cervical cancer cells by downregulating the FoxM1 expression

A novel microRNA, hsa-miR-6852 differentially regulated by Interleukin-27 induces necrosis in cervical cancer cells by downregulating the FoxM1 expression

IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells

ROS systems are a new integrated network for sensing homeostasis and alarming stresses in organelle metabolic processes

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