Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model

Abstract

Background Allergic asthma is a chronic airway inflammatory disease with a number of cytokines participating in its pathogenesis and progress. Interleukin (IL)-22, which is derived from lymphocytes, acts on epithelial cells and play a role in the chronic airway inflammation. However, the actual role of IL-22 in allergic asthma is still unclear. Therefore, we explored the effect of IL-22 on allergic airway inflammation and airway hyperresponsiveness (AHR) in an ovalbumin (OVA)-induced asthma mouse model. Methods To evaluate the effect of IL-22 in an allergic asthma model, BALB/c mice were sensitized and challenged with OVA; then the recombinant mouse IL-22 was administered intranasally 24 h prior to each challenge. The IL-22 levels in lung homogenates and bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay, respectively. AHR was evaluated through indicators including airways resistance (Rrs), elastance (Ers) and compliance (Crs); the inflammatory cell infiltration was assessed by quantification of differential cells counts in BALF and lung tissues stained by hematoxylin and eosin (H&E); IL-22 specific receptors were determined by immunohistochemistry staining. Results The concentration of IL-22 was significantly elevated in the OVA-induced mice compared with the control mice in lung homogenates and BALF. In the OVA-induced mouse model, IL-22 administration could significantly attenuate AHR, including Rrs, Ers and Crs, decrease the proportion of eosinophils in BALF and reduce inflammatory cell infiltration around bronchi and their concomitant vessels, compared with the OVA-induced group. In addition, the expression of IL-22RA1 and IL-10RB in the lung tissues of OVA-induced mice was significantly increased compared with the control mice, while it was dramatically decreased after the treatment with IL-22, but not completely attenuated in the IL-22-treated mice when compared with the control mice. Conclusion Interleukin-22 could play a protective role in an OVA-induced asthma model, by suppressing the inflammatory cell infiltration around bronchi and their concomitant vessels and airway hyperresponsiveness, which might associate with the expression of its heterodimer receptors. Thus, IL-22 administration might be an effective strategy to attenuate allergic airway inflammation.

Chemical Characterization and Potential Mechanism of the Anti-Asthmatic Activity of a Subfraction from Schisandra chinensis Fruit Extract

“…The structure of the isolated compounds was confirmed by HRESIMS and NMR data analysis. Compared with the literature data, these compounds were identified as pregomisin (3), 24 benzoylgomisin H (5), 25 gomisin O (7), 26 gomisin G (9), 27 (7S,8S,R-biar)-6,6,7,8tetrahydro-12,13-methylenedioxy-1,2,3,14-tetramethoxy-7,8dimethyldibenzo[a,c]cycloocten-9-one (10), 28 gomisin K 1 (11), 29 schisantherin B (12), 30 schisantherin A (13), 30 schisantherin D (15), 30 schsanhenol (17), 27 gomisin L 1 (18), 31 gomisin M 1 (21), 31 gomisin M 2 (22), 31 and schisandrin A (23). 30 Compound 10 was identified in S. chinensis for the first time.…”

“…Then, the tissues were embedded in paraffin wax and sectioned into 4–6 μm for hematoxylin–eosin (H&E) staining. The morphological structure and inflammatory state of the lung tissues were observed using CaseViewer software (3DHISTECH, Hungary) as previously described. , Lung sections were scored on a scale of 0–4 (0, no inflammatory cells; 1, mild inflammatory cells in the bronchial or vascular wall; 2, a ring of inflammatory cells in the bronchial or vascular wall; 3, two–four cell layers of inflammatory cells in the bronchial or vascular wall; 4, severe inflammation with diffuse inflammatory cells, more than four cell layers of inflammatory cells in the bronchial or vascular wall).…”

The Roles of IL-22 and Its Receptor in the Regulation of Inflammatory Responses in the Brain

“…The cell surface-standing IL-22 receptor complex consists of the IL-22Rα and IL-10Rβ receptor chains [ 5 ]. IL-10Rβ is expressed in all cells, including immune cells, whereas IL-22Rα is reportedly expressed in non-hematopoietic cells of the skin, kidney, intestine, liver, and pancreas [ 5 , 10 , 43 ]. To our knowledge, there have been no investigations of IL-22Rα expression in the brain; therefore, our current study examined IL-22 function and IL-22Rα expression in the mouse brain.…”

Zeaxanthin attenuates OVA-induced allergic asthma in mice by regulating the p38 MAPK/β-catenin signaling pathway

“…The allergic asthma model was induced by ovalbumin (OVA) according to the previous reports. [18][19][20] Briefly, 10 μg OVA (01641, 97%-100% (HPLC), Sigma, St. Louis, MO, USA), as an allergen, and 2 mg aluminum hydroxide (239186, CAS: 21645-51-2, reagent grade, Sigma), as an adjuvant in 200 µl phosphate buffer saline (PBS, Solarbio, Beijing, China) were intraperitoneally injected into BALB/c nude mice for sensitization on day-0, day-7, and day-14. Subsequently, mice were intranasally exposed to 3.75 μg OVA in 50 μL PBS on day-22, day-24, and day-26.…”