Interleukin 20 receptor A expression in colorectal cancer and its clinical significance

Li, Rui; Yin, Honghao; Sun, Xuejing; Liu, Songyi; Wang, Ang; Wu, Ying; Yuan, Yuan; Gong, Yuehua; Xing, Chengzhong

doi:10.7717/peerj.12467

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…The upregulated RNA molecules in the death vs. resistance piglets were mainly involved in innate immune pathways and signal transduction pathways. Interleukins (ILs) play critical roles in immune cell activation and regulation and inflammatory responses ( 28 ). IL-1α and IL-1β trigger a pro-inflammatory response upon binding to IL-1R1 receptor complexes ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics yields valuable information regarding the response mechanisms of Chinese Min pigs infected with PEDV

Li,

Zhou,

Zhang

et al. 2023

Front. Vet. Sci.

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Porcine epidemic diarrhea virus (PEDV) causes porcine epidemic diarrhea (PED), a highly infectious disease, which has resulted in huge economic losses for the pig industry. To date, the pathogenic and immune response mechanism was not particularly clear. The purpose of this study was to investigate the pathogenic and immune responses of pigs infected with PEDV.In this study, 12 Min pigs were randomly selected without taking colostrum. At 3 days old, eight piglets were infected with 1 mL of PEDV solution (10 TCID50/ml), and the remaining four piglets were handled by 1 mL of 0.9% normal saline. Within the age of 7 days old, four piglets died and were considered as the death group. Correspondingly, four alive individuals were classified into the resistance group. Tissues of the duodenum, jejunum, ileum, colon, cecum, and rectum of piglets in the three groups were collected to measure the PEDV content. Additionally, the jejunum was used for the measurements and analyses of Hematoxylin-eosinstaining (HE), immunohistochemical sections, and transcriptomics. The phenotypes of Min piglets infected with PEDV showed that the viral copy numbers and jejunal damage had significant differences between the death and resistance groups. We also observed the transcriptome of the jejunum, and the differentially expressed (DE) analysis observed 6,585 DE protein-coding genes (PCGs), 3,188 DE long non-coding RNAs (lncRNAs), and 350 DE microRNAs (miRNAs), which were mainly involved in immune response and metabolic pathways. Furthermore, the specific expressed molecules for each group were identified, and 97 PCGs,108 lncRNAs, and 51 miRNAs were included in the ceRNA-regulated networks. By weighted gene co-expression network analysis (WGCNA) and transcription factor (TF) prediction, 27 significant modules and 32 significant motifs (E-value < 0.05) annotated with 519 TFs were detected. Of these TFs, 53 were DE PCGs. In summary, the promising key PCGs, lncRNAs, and miRNAs related to the pathogenic and immunological response of pigs infected with PEDV were detected and provided new insights into the pathogenesis of PEDV.

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Section: Discussionmentioning

confidence: 99%

Transcriptomics yields valuable information regarding the response mechanisms of Chinese Min pigs infected with PEDV

Li,

Zhou,

Zhang

et al. 2023

Front. Vet. Sci.

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“…SGG induced a rather weak pro-tumor transcriptional signature on normal colonic FHC cells but was still specific (few genes exhibiting altered transcriptional profile with a low fold change). All the most up-regulated genes ( IL-20 , CLK1 , SORBS2 , ERG1 , PIM1 , SNORD3A ) are involved in cancer development [ 41 , 43 , 44 , 46 , 48 ]. We postulate that activation of all these genes could contribute to pre-cancerous transformation of colonic cells on long-term.…”

Section: Discussionmentioning

confidence: 99%

“…hand. The 6 up-regulated genes (IL-20, CLK1, SORBS2, ERG1, PIM1, SNORD3A) are involved in cancer development [43][44][45][46][47][48]. Interleukin 20 (IL-20) is a cytokine assigned to the interleukin 10 family described as tumor promoting [49].…”

Section: Plos Onementioning

confidence: 99%

Transcriptome profiling of human col\onic cells exposed to the gut pathobiont Streptococcus gallolyticus subsp. gallolyticus

Pasquereau-Kotula,

du Merle,

Sismeiro

et al. 2023

PLoS ONE

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Streptococcus gallolyticus sp. gallolyticus (SGG) is a gut pathobiont involved in the development of colorectal cancer (CRC). To decipher SGG contribution in tumor initiation and/or acceleration respectively, a global transcriptome was performed in human normal colonic cells (FHC) and in human tumoral colonic cells (HT29). To identify SGG-specific alterations, we chose the phylogenetically closest relative, Streptococcus gallolyticus subsp. macedonicus (SGM) as control bacterium. We show that SGM, a bacterium generally considered as safe, did not induce any transcriptional changes on the two human colonic cells. The transcriptional reprogramming induced by SGG in normal FHC and tumoral HT29 cells was significantly different, although most of the genes up- and down-regulated were associated with cancer disease. Top up-regulated genes related to cancer were: (i) IL-20, CLK1, SORBS2, ERG1, PIM1, SNORD3A for normal FHC cells and (ii) TSLP, BHLHA15, LAMP3, ZNF27B, KRT17, ATF3 for cancerous HT29 cells. The total number of altered genes were much higher in cancerous than in normal colonic cells (2,090 vs 128 genes being affected, respectively). Gene set enrichment analysis reveals that SGG-induced strong ER- (endoplasmic reticulum) stress and UPR- (unfolded protein response) activation in colonic epithelial cells. Our results suggest that SGG induces a pro-tumoral shift in human colonic cells particularly in transformed cells potentially accelerating tumor development in the colon.

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“…2A displaying the six most up-regulated genes and two down-regulated genes clearly shows the existence of 2 groups: SGG on one hand and SGM and NT on the other hand. The 6 up-regulated genes (IL-20, CLK1, SORBS2, ERG1, PIM1, SNORD3A) are involved in cancer development [39][40][41][42][43][44]. Interleukin 20 (IL-20) is a cytokine assigned to the interleukin 10 family described as tumor promoting [45].…”

Section: In Depth Analysis Of the Transcriptomic Responses Of Normal ...mentioning

confidence: 99%

Transcriptome profiling of human colonic cells exposed to the gut pathobiontStreptococcus gallolyticussubsp.gallolyticus

Ewa,

du Merle,

Sismeiro

et al. 2023

Preprint

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Streptococcus gallolyticus sp. gallolyticus (SGG) is a gut pathobiont involved in the development of colorectal cancer (CRC). To decipher the contribution of SGG in tumor initiation and/or acceleration respectively, a global transcriptome was performed in normal colonic cells (FHC) and in tumoral colonic cells (HT29). To identify SGG-specific alterations, we chose the phylogenetically closest relative, Streptococcus gallolyticus subsp. macedonicus (SGM) as the control bacterium. We show that SGM, a bacterium generally considered as safe, did not induce any transcriptional changes on the two human colonic cells. The transcriptional reprogramming induced by SGG was significantly different in FHC and HT29 cells, with most of the up- and down-regulated genes associated with cancer disease. Top up-regulated genes related to cancer were: (i) IL-20, CLK1, SORBS2, ERG1, PIM1, SNORD3A for normal FHC cells and (ii) TSLP, BHLHA15, LAMP3, ZNF27B, KRT17, ATF3 for cancerous HT29 cells. SGG induces much stronger transcriptional changes in cancerous than in normal colonic cells (2090 vs genes 128). Gene set enrichment analysis reveals that SGG-induced strong ER- (endoplasmic reticulum) stress and UPR- (unfolded protein response) activation in colonic epithelial cells. Our results suggest that SGG induces a pro-tumoral shift in human colonic cells, particularly in transformed cells, potentially accelerating tumor development in the colon.

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Interleukin 20 receptor A expression in colorectal cancer and its clinical significance

Cited by 6 publications

References 24 publications

Transcriptomics yields valuable information regarding the response mechanisms of Chinese Min pigs infected with PEDV

Transcriptomics yields valuable information regarding the response mechanisms of Chinese Min pigs infected with PEDV

Transcriptome profiling of human col\onic cells exposed to the gut pathobiont Streptococcus gallolyticus subsp. gallolyticus

Transcriptome profiling of human colonic cells exposed to the gut pathobiontStreptococcus gallolyticussubsp.gallolyticus

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