Interleukin-2 Secretion by Transduced and Unselected BDL-2 Lymphoma Results in Increased Survival in Mice with Previously Established Disseminated Disease

Orchard, Paul J.; Katsanis, Emmanuel; Boyer, Michael W.; May, Chad; Mclvor, R. Scott; Blazar, Bruce R.

doi:10.1089/cbr.1996.11.155

Cited by 1 publication

(1 citation statement)

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“…IL-2 secreting cellular vaccines have repeatedly been used with partial success in both preclinical and clinical studies (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14). GM-CSF secreted by gene-engineered tumour cells has been found to be the most potent stimulatory factor among the ten cytokines tested (15) and it has since become the most frequently used cytokine in the construction of cell-based cancer vaccines.…”

Section: Introductionmentioning

confidence: 99%

Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony-stimulating factor: I. Derivation, genetic stability, oncogenicity and immunogenicity

2012

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Abstract. The highly oncogenic bcr-abl-transformed mouse (Balb/c) 12B1 cells were transfected with plasmids carrying genes for either mouse interleukin-2 (IL-2) or the mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and the gene for blasticidine resistance. From the transduced cells several clones widely differing in the production of either cytokine were isolated. For further experiments, clones with the highest secretion of the cytokines were selected. When administered subcutaneously to mice, the IL-2-secreting cell line was approximately hundred times less pathogenic than the parental cells. A portion of animals developed small, spontaneously regressing tumours and most of them became resistant to challenge with the parental cells. Cell populations from either solid tumours or from organs infiltrated by the tumour cells predominantly consisted of cells which did not produce IL-2 and had lost resistance to blasticidine. This indicated that the IL-2 secreting cells were genetically unstable in the course of their propagation in vivo. On the other hand, the GM-CSF-secreting cells were more pathogenic than the parental cells, induced extensive organ damage and remained genetically stable in the course of their growth in vivo. The pathogenicity of different GM-CSF secreting clones directly depended on the magnitude of production of this cytokine. When used in the form of inactivated vaccines, the GM-CSF-secreting cells were more immunogenic than the IL-2-secreting cells. In comparative experiments, similar results were obtained with GM-CSF-and IL-2-secreting cells derived from B210 cells, another bcr-abl transformed cell line.

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