Interleukin 2 Receptor Expression in Patients With Head and Neck Squamous Carcinoma: Effects of Thymosin  1 In Vitro

Wolf, Gregory T.; Hudson, Jack W.; Peterson, Karen A.; Poore, Judy; McClatchey, Kenneth D.

doi:10.1001/archotol.1989.01860350079019

Cited by 16 publications

(7 citation statements)

References 21 publications

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“…Early in the disease process, PBL respond normally to mitogens such as phytohemagglutinin (PHA), concanavalin A (Con A), or other stimuli, whereas with progression of cancer, these responses deteriorate 72,78–87 . Patients with early stage HNSCC have normal T‐cell counts and CD4:CD8 ratios in their peripheral blood, whereas patients with advanced disease have reduced counts, decreased numbers of CD4+ cells, and small reductions in CD8+ cells 77,78,88–94 …”

Section: Discussionmentioning

confidence: 99%

Covalent linking of proteins and cytokines to suture: Enhancing the immune response of head and neck cancer patients

et al. 2003

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Backgrwund:The immune system of advanced stage head and neck cancer patients is frequently suppressed. Poor immune function has been correlated with poor clinical outcome. Immunotherapeutic strategies have been previously attempted in an effort to enhance immune function and improve survival. Previous studies have shown surgical suture can be transformed into an immune stimulant capable of activating the T lymphocytes of cancer patients. The development of a process for covalently linking proteins and cytokines to suture could have enormous potential for the in vivo manipulation of the immune system. Hypothesis: We hypothesize proteins and cytokines can be covalently linked to surgical suture while preserving their functional properties. Study Design: Prospective study testing normal donor and head and neck squamous cell carcinoma (HNSCC) patient lymphocytes. Method. Polyester suture was acid hydrolyzed followed by reacting with l-ethyl-3(-3-dimethylamino propyl carbodiimide) (EDAC) to create a suture-EDAC intermediate. activity. The optimal conditions for linking 1C2 or m-7 were defined. The covalently linked cytokines retained their immune enhancing properties for stimulating PBL and lymph node lymphocytes (LNL) from HNSCC patients to proliferate, generate a TH1 immunologic profile of cytokines (IG2, IL-12, IFN-y), and stimulate T lymphocytes. Conclusion: This is the first report to demonstrate that cytokines can be covalently linked to surgical sutures and retain their immune-stimulating properties. Proteins linked to suture also retained their enzymatic activity. The clinical implications of functionally active cytokines or proteins linked to surgical suture may be very significant in the future for manipulating the immune system in vivo o r enhancing wound healing.

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Section: Discussionmentioning

confidence: 99%

Covalent linking of proteins and cytokines to suture: Enhancing the immune response of head and neck cancer patients

et al. 2003

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“…Serum levels are particularly high in newborns but drop gradually throughout childhood to adult levels between 540 and 670 pg/mL, thus suggesting a role in early immune development [9]. T␣1 was observed to stimulate lymphokine production of IFN-␥, IFN-␣, IL-2, and macrophage migration inhibitory factor (MIF) [10]. Further, T␣1 has been shown to induce a Th1-type response and antibody production [11].…”

Section: Introductionmentioning

confidence: 99%

Thymosin-α1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes

et al. 2007

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Although thymosins have been demonstrated to have immunomodulatory effects, it is still not clear whether they could affect dendritic cells (DCs), the most professional antigen-presenting cells. The objective of this study was to determine the effect and potential mechanisms of thymosin-alpha1 (Talpha1) on DC differentiation and functional maturation. Human peripheral blood CD14(+) monocytes were purified by using a magnetic separation column and cultured with GM-CSF and IL-4 to differentiate into immature DCs (iDCs). In the presence of Talpha1, iDC surface markers CD40, CD80, MHC class I and class II molecules were significantly upregulated as measured by flow cytemotry analysis. However, Tbeta4 or Tbeta10 did not show these effects on iDCs. There was an approximately 30% reduction in antigen (FITC-conjugated dextran)-uptake by Talpha1-treated iDCs as compared with non-Talpha1-treated iDCs. In addition, Talpha1-treated matured DCs (mDCs) showed an increased stimulation of allogeneic CD3(+) T-cell proliferation as measured by a mixed-lymphocyte reaction assay. Talpha1-treated mDCs also increased the production of several Th1- and Th2-type cytokines as measured by a Bio-Plex cytokine assay. Furthermore, rapid activation of p38 MAPK and NFkappaB was seen in Talpha1-treated iDCs as measured by a Bio-Plex phosphoprotein assay. Thus, Talpha1 significantly enhances DC differentiation, activation, and functions from human peripheral blood CD14(+) monocytes possibly through a mechanism of the activation of p38 MAPK and NFkappaB pathways. This study provides a basis to further evaluate Talpha1 as a possible adjuvant for a DC-directed vaccine or therapy.

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“…Data shown in B16F10 and H460 tumor models provide information about the change of tumor volume and body weight of C57BL/6 mice or BALB/c nude mice over time, also, the tumor weight of different groups on the last day. Tα1, as an immunomodulator used in cancer therapy [1] , has abilities to induce the activation of DCs and T cells as well as the secretion of interferon-gamma (IFN-γ) and interleukin-2 (IL-2) [2] . IFN-γ is important in the immune system stems for its immunomodulatory activity, whereas IL-2 can promote the differentiation of T cells into effector T cells and boost the host immunity against cancer [3] , [4] .…”

Section: Datamentioning

confidence: 99%

Effects on tumor growth and immunosuppression of a modified Tα1 peptide along with its circular dichroism spectroscopy data

Wang

et al. 2018

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The data presented in this article are related to the research article entitled “Immunomodulatory and Enhanced Antitumor Activity of a Modified Thymosin α1 in Melanoma and Lung Cancer” (Wang et al., 2018). Tα1 has been evaluated as effective in cancer treatment. In order to make it capable to target tumor, a peptide iRGD was introduced to Tα1. The anti-tumor activity was accessed by constructing in vivo melanoma and human non-small-cell lung cancer models treated with Tα1-iRGD to measure the tumor volume over time and tumor weight at the last day. The concentration of IFN-γ and IL-2 in C57BL/6 mice peripheral blood was determined by ELISA. And the immunomodulatory ability of Tα1-iRGD was evaluated in vivo by thymus index and spleen index. Those functions this paper was aimed at may have relationship with its secondary structure, so the circular dichroism spectra of Tα1, iRGD and Tα1-iRGD was performed.

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Interleukin 2 Receptor Expression in Patients With Head and Neck Squamous Carcinoma: Effects of Thymosin 1 In Vitro

Cited by 16 publications

References 21 publications

Covalent linking of proteins and cytokines to suture: Enhancing the immune response of head and neck cancer patients

Covalent linking of proteins and cytokines to suture: Enhancing the immune response of head and neck cancer patients

Thymosin-α1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes

Effects on tumor growth and immunosuppression of a modified Tα1 peptide along with its circular dichroism spectroscopy data

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