Interleukin-2 and interleukin-10 gene expression in calves experimentally infected with Fasciola gigantica

Ingale, S.L.; Singh, Putan; Raina, O.K.; Verma, A. K.; Channappanavar, Rudragouda; Mehra, U. R.

doi:10.1016/j.livsci.2010.03.002

Cited by 6 publications

(2 citation statements)

References 13 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, this coordinated response may be mediated by the parasite and/or the host in order to promote parasite survival while minimizing host hepatic damage. Th2-predominant response together with the suppression of Th1/Th17 response has been reported previously [ 33 – 36 ]. Interestingly, some molecules secreted by Fasciola species can suppress the differentiation of Th17 cells independently of Th2 cells differentiation, by altering the function of dendritic cells [ 37 ].…”

Section: Discussionsupporting

confidence: 59%

Dynamic expression of cytokine and transcription factor genes during experimental Fasciola gigantica infection in buffaloes

et al. 2017

View full text Add to dashboard Cite

BackgroundDetermining the mechanisms involved in the immune-pathogenesis of the tropical liver fluke, Fasciola gigantica, is crucial to the development of any effective therapeutic intervention. Here, we examined the differential gene expression of cytokines and transcription factors in the liver of F. gigantica-infected buffaloes, over the course of infection.MethodsWater buffaloes (swamp type) were infected orally with 500 F. gigantica encysted metacercariae. Liver tissue samples were collected 3, 10, 28, 42, 70 and 98 days post-infection (dpi). Levels of gene expression of nine cytokines (IFN-γ, TGF-β, IL-1β, IL-4, IL-6, IL-10, IL-12B, IL-13 and IL-17A) and four transcription factors (T-bet, GATA-3, Foxp3 and ROR-γτ) were determined using quantitative real-time PCR (qRT-PCR). We evaluated any correlation between gene expression of these immune-regulatory factors and the severity of liver pathology.ResultsHistopathological examination revealed that cellular infiltration, hemorrhage and fibrosis without calcification in the liver parenchyma of infected buffaloes, increased over the course of infection. This progressive pathology was attributed to dysregulated and excessive inflammatory responses induced by infection. The early infection phase (3–10 dpi) was marked by a generalized immunosuppression and elevated TGF-β expression in order to facilitate parasite colonization. A mixed Th1/Th2 immune response was dominant from 28 to 70 dpi, to promote parasite survival while minimizing host tissue damage. During late infection (98 dpi), the response was biased towards Th1/Treg in order to inhibit the host’s Th2 protective response and promote chronic infection. Both IL-10 and IL-17A and the Th17/Treg balance, played key roles in mediating the inflammatory and immunoregulatory mechanisms in the liver during chronic fasciolosis.ConclusionsOur data showed distinct CD4+ T helper (Th) polarization and cytokine dysregulation in response to F. gigantica infection in water buffaloes over the course of infection. Characterizing the temporal expression profiles for host immune genes during infection should provide important information for defining how F. gigantica adapts and survives in the liver of buffaloes and how host immune responses influence F. gigantica pathogenicity.

show abstract

Section: Discussionsupporting

confidence: 59%

Dynamic expression of cytokine and transcription factor genes during experimental Fasciola gigantica infection in buffaloes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The immune response induced during natural infection has been well characterized in ruminants for F. hepatica although there are increasing studies on F. gigantica that report similar findings . During the acute stages of infection, cattle exhibit a mixed immune response with elevated IL‐10, TGF‐β, IL‐4 and IFN‐γ.…”

Section: Immune Responses and Immunity To Liver Flukementioning

confidence: 99%

A prospective view of animal and human Fasciolosis

Cwiklinski

O’Neill

Donnelly

et al. 2016

Parasite Immunology

167

114

View full text Add to dashboard Cite

SummaryFasciolosis, a food‐borne trematodiasis, results following infection with the parasites, Fasciola hepatica and Fasciola gigantica. These trematodes greatly affect the global agricultural community, infecting millions of ruminants worldwide and causing annual economic losses in excess of US $3 billion. Fasciolosis, an important zoonosis, is classified by WHO as a neglected tropical disease with an estimated 17 million people infected and a further 180 million people at risk of infection. The significant impact on agriculture and human health together with the increasing demand for animal‐derived food products to support global population growth demonstrate that fasciolosis is a major One Health problem. This review details the problematic issues surrounding fasciolosis control, including drug resistance, lack of diagnosis and the threat that hybridization of the Fasciola species poses to future animal and human health. We discuss how these parasites may mediate their long‐term survival through regulation and modulation of the host immune system, by altering the host immune homeostasis and/or by influencing the intestinal microbiome particularly in respect to concurrent infections with other pathogens. Large genome, transcriptome and proteomic data sets are now available to support an integrated One Health approach to develop novel diagnostic and control strategies for both animal and human disease.

show abstract

Allele substitution and dominance effects of CD166/ALCAM gene polymorphisms for endoparasite resistance and test-day traits in a small cattle population using logistic regression analyses

et al. 2019

View full text Add to dashboard Cite

Interleukin-2 and interleukin-10 gene expression in calves experimentally infected with Fasciola gigantica

Cited by 6 publications

References 13 publications

Dynamic expression of cytokine and transcription factor genes during experimental Fasciola gigantica infection in buffaloes

Dynamic expression of cytokine and transcription factor genes during experimental Fasciola gigantica infection in buffaloes

A prospective view of animal and human Fasciolosis

Allele substitution and dominance effects of CD166/ALCAM gene polymorphisms for endoparasite resistance and test-day traits in a small cattle population using logistic regression analyses

Contact Info

Product

Resources

About