Interleukin-1β secretion in hippocampal sclerosis patients with mesial temporal lobe epilepsy

Abstract

Mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) is a common medically intractable epilepsy syndrome. Although pathogenesis of HS still remains highly controversial, genetics may play a role as a predisposing factor. Previous evidence in a Japanese population revealed that the homozygotes for allele T at position -511 of the interleukin (IL)-1β gene promoter region (IL-1β-511 T/T) confers susceptibility to the development of HS. However, whether this polymorphism has an effect on IL-1β levels in MTLEHS patients was not demonstrated. This study aimed to analyze the distribution of this particular polymorphism in a group of Turkish HS patients and correlate the polymorphism with IL-1β secretion from the lymphocytes, thus revealing a functional role for IL-1β in the etiopathogenesis of HS. A single base pair polymorphism at position –511 in the promoter region of the IL-1β gene was analyzed. The spontaneous and 1 ng/mL lipopolysaccharidestimulated production of IL-1β by peripheral blood mononuclear cells after 4 and 24 h of incubation were measured by ELISA method. The heterozygous type (–511 C/T) was the most common genotype. There was no difference in frequency of allele –511 T between patients and controls. Analysis of IL-1β levels, genotype and allele distributions showed no significant difference among the groups (P>0.05). Nevertheless, it was seen that patients who carry a T allele at position -511 of the IL-1β gene had increased IL-1β levels. T-allele carriage may be important. Only IL-1β secretion from the lymphocytes has been assessed in this study. Considering the importance of IL-1β in the etiopathogenesis of HS, further studies are needed to evaluate locally produced IL-1β levels.

Agmatine Alleviates Epileptic Seizures and Hippocampal Neuronal Damage by Inhibiting Gasdermin D-Mediated Pyroptosis

“…It has been reported that GSDMD is activated by the NLRP3 inflammasome, which then cleaves GSDMD to produce GSDMD-N, and GSDMD-N is translocated to the plasma membrane to form a pore; then, the cell membrane swells and ruptures, resulting in the release of cytoplasmic contents and the leakage of mature IL-1β and IL-18 (Ding et al, 2016). Studies have shown that the levels of IL-1β are elevated in dogs with epilepsy (Kostic et al, 2019) and in hippocampal sclerosis patients with mesial temporal lobe epilepsy (Dundar et al, 2013). We found that agmatine could reduce the expression of NLRP3, ASC, caspase-1 and IL-1β at the protein and mRNA levels, and inhibit the release of IL-1β in serum and supernatant, thus inhibiting neuroinflammation, GSDMD activation, and pyroptosis.…”

From kitchen to clinic: Pharmacotherapeutic potential of common spices in Indian cooking in age-related neurological disorders

“…The role of inflammation and oxidative stress in the pathogenesis and exacerbation of disease should not be overlooked. IL-1B is known to be an important inflammatory marker and is found to induce epilepsy by inducing inflammation in the brain ( Olgac Dundar et al, 2013 ). The increased levels of IL-1B could also flare an aggravated inflammatory response including TNF-α release, which itself is able to regulate the synaptic transmission, thus affecting the neural system excitability ( Kumar et al, 2007 ; Tiwari et al, 2012 ).…”

Association of TNF-alpha, IL-6 and IL-1beta gene polymorphisms with polycystic ovary syndrome: a meta-analysis

“…The IL-1 gene cluster on chromosome 2q12-13 contains three related genes, IL-1alpha , IL-1beta , and IL-1RN . A common C/T SNP of the IL-1beta promoter at position −511 has been found to correspond with altered IL-1beta protein expression both in vitro and in vivo [ 13 ].…”