Interleukin-1β Induces Interleukin-lα Messenger Ribonucleic Acid Expression in Primary Cultures of Leydig Cells*

Wang, Deli; Nagpal, Madan L.; Calkins, Jo H.; Chang, Weiwei; Sigel, M. Michael; Lin, T.

doi:10.1210/endo-129-6-2862

Cited by 50 publications

(21 citation statements)

References 21 publications

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“…For example, both IL-la and IL-lß mRNA levels in mononuclear cells are increased after a 4-h exposure to recombinant IL-1 (Warner et al 1987). IL-1 was also found to increase IL-1 mRNA expression in primary cultures of Leydig cells (Wang et al 1991). This suggests that cytokine-induced cytokine expression may be a common feature of autocrine regulation.…”

Section: Discussionmentioning

confidence: 78%

Ontogeny of the expression and regulation of interleukin-6 (IL-6) and IL-1 mRNAs by human trophoblast cells during differentiation in vitro

Stephanou

Myatt

Eis

et al. 1995

Journal of Endocrinology

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During human placental differentiation, mononuclear cytotrophoblast cells fuse and differentiate into syncytiotrophoblast cells. Although syncytiotrophoblast cells have been shown to express interleukin-1 alpha (IL-1 alpha), IL-1 beta and IL-6, the pattern of expression of these cytokines during placental differentiation is unknown. We have examined the expression of IL-1 alpha, IL-1 beta and IL-6 mRNA during differentiation of cytotrophoblast cells in culture. IL-1 alpha, IL-1 beta and IL-6 mRNA levels were determined by semiquantitative reverse transcription-PCR analysis using glyceraldehyde phosphate dehydrogenase as an internal control. All three cytokine mRNA levels decreased markedly during trophoblast differentiation. After 6 days in culture, when almost all the cytotrophoblast cells had fused and differentiated into syncytiotrophoblast cells, the amounts of IL-1 alpha, IL-1 beta and IL-6 mRNA were decreased by 87.1, 72.1 and 60.9% respectively. Exogenous IL-6 had differential effects on cytokine mRNA expression. When added to placental cultures during the first 6 days of culture, IL-6 markedly inhibited IL-6, IL-1 alpha and IL-1 beta mRNA expression. However, when added to the cells during days 6-9 of culture, when most of the cells were syncytiotrophoblast cells, IL-6 stimulated IL-1 alpha and IL-1 beta mRNA expression. The results of these studies indicate that IL-1 alpha, IL-1 beta and IL-6 mRNA expression decreases markedly during cytotrophoblast differentiation in vitro and that the regulation of trophoblast cytokine mRNA levels changes during differentiation.

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Section: Discussionmentioning

confidence: 78%

Ontogeny of the expression and regulation of interleukin-6 (IL-6) and IL-1 mRNAs by human trophoblast cells during differentiation in vitro

Stephanou

Myatt

Eis

et al. 1995

Journal of Endocrinology

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“…Leydig cells express macrophage inhibitory factor constitutively (43), and IL-1␤, IL-1␣, and IL-6 on stimulation with the inflammatory stimulus lipopolysaccharide (LPS) in vitro (39,51,67). Isolated Sertoli cells produce IL-1␣ constitutively and IL-6 after stimulation with either LPS or exogenous IL-1 (12,18,51).…”

mentioning

confidence: 99%

Cytokine profiles in the testes of rats treated with lipopolysaccharide reveal localized suppression of inflammatory responses

O'Bryan¹,

Gerdprasert²,

Nikolic-Paterson³

et al. 2005

American Journal of Physiology-Regulatory, Integrative and Comp

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“…I IL-1 receptor was demonstrated (43). This report may indicate that IL-1, which is constitutively expressed (physiological levels) in testicular tissues (11)(12)(13)(14)(15)(16)(17)(18)(19)(20) and was shown to increase germ cell proliferation and DNA synthesis (44,45), is not a limiting factor in steroidogenesis and spermatogenesis under physiological conditions. It is possible that other cytokines (such as TNF-␣) may subjugate the role of IL-1.…”

Section: Discussionmentioning

confidence: 88%

“…Further investigations have identified several cellular sources of IL-1 in the testis. In the interstitium, Leydig cells (13)(14)(15) and testicular macrophages (16) were found to produce and secrete IL-1, preferably the ␤-form. IL-1␣ production can be detected in tubular sources, namely Sertoli cells (13,14,(17)(18)(19) and germ cells (17,20).…”

mentioning

confidence: 99%

Effect of Interleukin-1 Receptor Antagonist Gene Deletion on Male Mouse Fertility

et al. 2008

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Members of the IL-1 family are pleiotropic cytokines that are involved in inflammation, immunoregulation, and other homeostatic functions in the body. IL-1alpha, IL-1beta, and the IL-1 antagonistic molecule [IL-1 receptor antagonist (IL-1 Ra)] are present in the testis under normal homeostasis, and they further increase upon infection/inflammation. In the present study, we examined the effect of IL-1 Ra gene deletion on male mouse fertility. Male mice [wild type (WT) and IL-1 Ra knockout (KO)] were mated with WT females, and the birth and number of offspring were recorded 21-45 d after mating. Furthermore, the concentration, motility, and morphology of sperm isolated from the cauda of the epididymis were evaluated. The ability of the calcium ionophore (A23187) to induce acrosome reaction (AR) in the sperm of WT and IL-1 Ra KO mice was compared with their ability to fertilize in vitro oocytes from WT females. The direct effect of IL-1alpha and IL-1beta on AR and abnormal morphology in sperm from WT were evaluated. The levels of IL-1alpha and IL-1beta in the testes of WT and IL-1 Ra KO mice were examined by specific ELISA and real-time PCR. Our results show a significant reduction in the capacity of IL-1 Ra KO male mice to fertilize WT females (P < 0.05). Furthermore, the number of offspring in mice fertilized with IL-1 Ra KO male mice was significantly lower than with WT males (P < 0.05). Sperm concentration and the percentage of motile sperm from IL-1 Ra KO and WT were similar; however, the percentage of sperm with abnormal morphology (mainly in the head) and acrosome-reacted sperm cells were significantly higher in the IL-1 Ra KO, compared with that of WT males (P < 0.05). In vitro, the ability of sperm from IL-1 Ra KO male mice to fertilize oocytes from WT females was significantly lower than sperm from WT mice (P < 0.05). In addition, the percentage of reacted sperm from IL-1 Ra KO, spontaneously without ionophore induction, was significantly higher than from WT (P < 0.05). Sperm from WT underwent induction of AR only by ionophore; however, sperm from IL-1 Ra KO were unable to undergo the AR by ionophore, indicating that they are induced and, thus, are inactive in fertilization. Testicular IL-1alpha and IL-1beta levels were significantly higher in IL-1 Ra KO, compared with WT male mice (P < 0.05). The addition of recombinant IL-1alpha or IL-1beta to sperm from a WT mouse induced their AR, and significantly increased abnormal sperm morphology, as compared with controls (P < 0.05). This effect was neutralized by the addition of IL-1 Ra. Our results indicate the involvement of IL-1 in sperm physiology, affecting its morphology and fertilization ability. Higher than homeostatic levels of IL-1 in the testis, as observed in IL-1 Ra KO mice, impaired the ability of sperm to fertilize oocytes. Together, these results may explain some of the male infertility cases with an infection/inflammation background and may hint at the ability to use IL-1 Ra in future therapeutic strategies in these cases.

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Interleukin-1β Induces Interleukin-lα Messenger Ribonucleic Acid Expression in Primary Cultures of Leydig Cells*

Cited by 50 publications

References 21 publications

Ontogeny of the expression and regulation of interleukin-6 (IL-6) and IL-1 mRNAs by human trophoblast cells during differentiation in vitro

Ontogeny of the expression and regulation of interleukin-6 (IL-6) and IL-1 mRNAs by human trophoblast cells during differentiation in vitro

Cytokine profiles in the testes of rats treated with lipopolysaccharide reveal localized suppression of inflammatory responses

Effect of Interleukin-1 Receptor Antagonist Gene Deletion on Male Mouse Fertility

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