Interleukin‐1β expression is increased in the duodenum of dogs with idiopathic inflammatory bowel disease

Hawes, Martin; Riddle, Anna; Kirk, Joseph; Jergens, Albert E.; Allenspach, Karin

doi:10.1136/vr.104495

Cited by 7 publications

(11 citation statements)

References 9 publications

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“…In human IBD, elevations of IL-1β levels are associated with increased disease severity [ 18 , 19 ]. In canine IBD, findings regarding the correlation of IL-1β mRNA or protein levels with disease severity are controversial [ 6 , 20 , 21 , 40 , 45 ]. In our study there was no significant correlation between the relative IL-1β mRNA expression in the colonic mucosa and the CCECAI, colonoscopy or the histopathologic parameters score in dogs with large intestinal IBD.…”

Section: Discussionmentioning

confidence: 99%

“…Controversial findings regarding the correlation of IL-1β protein levels and disease severity in canine IBD have also been reported. More specifically, Hawes et al reported a moderate positive correlation between IL-1β protein expression in the duodenum mucosa and CCECAI score in dogs with IBD [ 20 ]. Conversely, in a recent study examing the protein levels of IL-1β and IL-1 receptor antagonist (IL-1Ra) in the colonic mucosa of dogs with IBD, did not observe a significant correlation between the IL-1β:IL-1Ra ratio and CCECAI score [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Increased mucosal expression and production of IL-1ß has been found in Crohn's disease and ulcerative colitis, as well as in animal models of colitis [35][36][37][38][39]. IL-1β has also been implicated in canine IBD pathogenesis [20][21][22]. Its expression has been examined in the duodenal, and to a lesser extent in the colonic mucosa of dogs with IBD [4,6,21,40].…”

Section: Plos Onementioning

confidence: 99%

“…inflammatory protease caspase-1, before it is secreted as the active IL-1β [44]. Recent studies have investigated IL-1β protein levels in the intestinal and colonic mucosa in canine IBD, using enzyme-linked immunosorbent assay (ELISA) or immunohistochemistry (IHC) [20][21][22][23][24]. Increased IL-1β protein levels in the duodenal mucosa of dogs with IBD has been reported repeatedly.…”

Section: Plos Onementioning

confidence: 99%

“…More specifically, IL-1β is a proinflammatory cytokine that plays an important role in the pathogenesis of intestinal inflammation in human IBD with elevations of IL-1β levels being associated with increased IBD severity [ 18 , 19 ]. Previous studies in canine IBD have shown increased IL-1β mRNA expression and protein levels, as well as increased IL-1β/ IL-1Ra protein ratio in the duodenal and/or colonic mucosa of dogs with IBD compared to healthy controls [ 20 – 24 ]. IL-2 and TNF-α expression has been previously studied in canine IBD using different methods with conflicting results [ 4 – 7 , 9 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Colonic mucosal and cytobrush sample cytokine mRNA expression in canine inflammatory bowel disease and their correlation with disease activity, endoscopic and histopathologic score

et al. 2021

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Canine inflammatory bowel disease (IBD) is a group of chronic gastrointestinal disorders, the pathogenesis of which remains elusive, but it possibly involves the interaction of the intestinal immune system with luminal microbiota and food-derived antigens. Mucosal cytokines profiles in canine IBD have been investigated mainly in small intestinal disease, while data on cytokine profiles in large intestinal IBD are limited. The objective of this study was to measure colonic mucosal and cytobrush sample messenger (m)RNA expression of interleukin (IL)-1β, IL-2, IL-12p40, IL-23p19, tumor necrosis factor-alpha (TNF-α) and chemokine C‐C motif ligand (CCL28) in dogs with IBD and healthy controls using quantitative real-time polymerase chain reaction (PCR), and assess their correlation with clinical disease activity, endoscopic and histopathologic score. Dogs with IBD had a significantly increased mRNA expression of IL-1β, IL-23p19 and CCL28 in the colonic mucosa, compared to healthy controls. None of the selected cytokines had significantly different mRNA expression in the colonic cytobrush samples between the two groups or between the colonic mucosa and cytobrush samples of dogs with IBD. Finally, there was a statistically significant correlation of clinical disease activity with endoscopic activity score and fibrosis and atrophy of the colonic mucosa in dogs with large intestinal IBD. IL-1β, IL-23p19 and CCL28 could play a role in the pathogenesis of canine large intestinal IBD. Colonic cytokine expression does not correlate with clinical disease activity and/or endoscopic score. However, clinical signs reflect the severity of endoscopic lesions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Colonic mucosal and cytobrush sample cytokine mRNA expression in canine inflammatory bowel disease and their correlation with disease activity, endoscopic and histopathologic score

et al. 2021

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Silencing interleukin 1α underlies a novel inhibitory role of miR‐181c‐5p in alleviating low‐grade inflammation of rats with irritable bowel syndrome

Zhao

et al. 2019

J of Cellular Biochemistry

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Irritable bowel syndrome (IBS) is a common disorder of unknown etiology. Studies have found a close relation between IBS and microRNAs (miRNAs), but the study concerning the relationship between IBS and miR‐181c‐5p in IBS is still blank. Thus, this study aims to explore the role of miR‐181c‐5p in IBS via interleukin 1α (IL1A). Initially, microarray analysis was used to retrieve the genes related to IBS and to predict miRNAs regulating IL1A gene. IBS model was then established with abdominal withdraw reflection (AWR) and Bristol stool grading in mice measured. Afterwards, the functional role of miR‐181c‐5p in IBS was determined using the ectopic expression, depletion and reporter assay experiments, as well as miR‐181c‐5p and IL1A expression detected. Subsequently, expression of tumor necrosis factor‐α (TNF‐α), interleukin‐2 (IL‐2), and IL‐6 were detected to further determine the effects of miR‐181c‐5p and IL1A on inflammation in IBS. miR‐181c‐5p and IL1A might be involved in IBS. miR‐181c‐5p was found to be decreased while IL1A was increased in IBS rats. In addition, miR‐181c‐5p could target and inhibit expression of IL1A, and IBS mice exhibited elevated AWR and Bristol stool grading, namely 6 to 7 points (70.4 [38 of 54]). Moreover, with the overexpression of miR‐181c‐5p or silencing of IL1A, the expression of TNF‐α, IL‐2, and IL‐6 was decreased. Collectively, this study suggested that overexpressed miR‐181c‐5p could silence IL1A, thus inhibiting low‐grade inflammation in IBS rats. miR‐181c‐5p/IL1A is expected to serve as a novel target for the treatment of IBS.

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Presence of the house dust mite allergen in the gastrointestinal tract of dogs with chronic enteropathy: A potential inducer of interleukin-1β

Takahashi

Yanuma

Hirokawa

et al. 2020

Veterinary Immunology and Immunopathology

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Interleukin‐1β expression is increased in the duodenum of dogs with idiopathic inflammatory bowel disease

Cited by 7 publications

References 9 publications

Colonic mucosal and cytobrush sample cytokine mRNA expression in canine inflammatory bowel disease and their correlation with disease activity, endoscopic and histopathologic score

Colonic mucosal and cytobrush sample cytokine mRNA expression in canine inflammatory bowel disease and their correlation with disease activity, endoscopic and histopathologic score

Silencing interleukin 1α underlies a novel inhibitory role of miR‐181c‐5p in alleviating low‐grade inflammation of rats with irritable bowel syndrome

Presence of the house dust mite allergen in the gastrointestinal tract of dogs with chronic enteropathy: A potential inducer of interleukin-1β

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