Interleukin‐1β, clinical parameters and matched cellular‐histopathologic changes of biopsied gingival tissue from periodontitis patients

Hou, Linlin; Liu, Cheing-Meei; Liu, Bu-Yuan; Lin, Shih-Jung; Liao, Chia-Shih; Rossomando, Edward F.

doi:10.1034/j.1600-0765.2003.02601.x

Cited by 70 publications

(62 citation statements)

References 46 publications

(92 reference statements)

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“…Estas células liberan citocinas, como interleucina 1 (IL-1), interleucina 6 (IL-6), factor nuclear kappa beta (NF-κβ), factor de necrosis tumoral alfa (TNF-α) (3)(4)(5), rank ligando (RANK-L) y prostaglandina E2 (PGE2), que inducen la pérdida ósea (6). La respuesta inflamatoria local resultante de la periodontitis puede desencadenar una respuesta sistémica inflamatoria y constituirse en un factor de riesgo para patologías como enfermedad cardiovascular, diabetes, artritis y osteoporosis (7).…”

Section: Introductionunclassified

Condición periodontal de pacientes con apnea obstructiva del sueño / Periodontal Status of Patients with Obstructive Sleep Apnea

et al. 2016

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RESUMENAntecedentes: Existen patologías sistémicas que tienen relación directa con la enfermedad periodontal; por esto es necesario identificarlas para controlarlas y así evitar que esta condición se agrave. Objetivo: Describir la condición periodontal de los pacientes que asistieron a un estudio polisomnográfico. Métodos: Este fue un estudio descriptivo de corte transversal. La muestra incluyó 166 pacientes con estudio polisomnográfico previo a quienes se les realizó el examen periodontal para determinar su diagnóstico periodontal fundamentado en la clasificación de Armitage, de 1999, y subclasificado de acuerdo con la localización. Resultados: De los 166 pacientes evaluados, el 99,40 % presentó afectación periodontal, de quienes el 71,08 % presentó síndrome de apnea/hipoapnea del sueño (SAHOS); el 58,4 %, periodontitis, y el 36,74 %, gingivitis. Las patologías de mayor prevalencia correspondieron al 60,39 % del total de la muestra y fueron gingivitis inducida por biopelícula con pérdida de inserción preexistente, periodontitis crónica severa localizada y periodontitis crónica moderada localizada. Afectaron a los pacientes con el diagnóstico de SAHOS en un 41,11 % y en el 19,28 % restante a los pacientes sin SAHOS. Conclusiones: El diagnóstico de mayor prevalencia en los pacientes que asistieron al examen polisomnográfico fue gingivitis con pérdida de inserción preexistente generalizada. El diagnóstico de periodontitis en pacientes con SAHOS estuvo más relacionado con la presencia de factores locales. A mayor severidad del diagnóstico periodontal, menor número de dientes presentes en boca, mayor movilidad, mayor cantidad de furcas afectadas, menor frecuencia de cepillado y mayor edad.PALABRAS CLAVE apnea obstructiva del sueño; enfermedad periodontal; hipoapnea obstructiva del sueño; periodontitis; síndrome de apnea o hipoapnea obstructiva del sueño (SAHOS) ÁREAS TEMÁTICAS neumología; otorrinolaringología; periodoncia ABSTRACT Background: There are systemic diseases that are directly related to periodontal disease, so it is necessary to identify them, in order to control them and prevent the worsening of this condition. Objective: To describe the periodontal condition of patients who underwent polysomnographic analysis. Methods: This was a cross-sectional study. The sample included 166 patients with prior polysomnography, who underwent periodontal examination to determine periodontal diagnosis based on the classification of Armitage 1999 and sub-classified according to location. Results: Of the 166 patients evaluated, 99.40 % had periodontal diseases, of which 71.08 % had obstructive sleep apnea/hypopnea syndrome (OSA), periodontitis 58.4% and gingivitis 36.74 %. The most prevalent periodontal diseases corresponded to 60.39 % of the total sample and gingivitis with preexisting insertion loss, severe chronic periodontitis, and moderate chronic periodontitis affected 41.11 % of patients with the diagnosis of OSA and remaining 19.28 % did not have OSA. Conclusions: The most prevalent diagnosis in patients with poly...

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Section: Introductionunclassified

Condición periodontal de pacientes con apnea obstructiva del sueño / Periodontal Status of Patients with Obstructive Sleep Apnea

et al. 2016

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“…Both macrophages and gingival fibroblasts are present in periodontitis-inflamed periodontal tissue (16,17), and their interaction has been shown to increase MMP expression (18,19). However, the underlying mechanism has not been investigated.…”

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Interleukin-6 Released from Fibroblasts Is Essential for Up-regulation of Matrix Metalloproteinase-1 Expression by U937 Macrophages in Coculture

Sundararaj

Samuvel

et al. 2009

Journal of Biological Chemistry

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Matrix metalloproteinases (MMPs) play a key role in periodontal disease. Although it is known that macrophages and fibroblasts are co-localized and express MMPs in the diseased periodontal tissue, the effect of interaction between these two cell types on MMP expression has not been well elucidated. Furthermore although it is known that diabetes is associated with accelerated periodontal tissue destruction, it remains unknown whether hyperglycemia, a major metabolic abnormality in diabetes, regulates MMP expression by affecting the cross-talking between fibroblasts and macrophages. In this study, human gingival fibroblasts and U937 macrophages were cocultured in a two-compartment transwell culture system, and the cells were treated with normal or high glucose. We found that coculture of fibroblasts and U937 macrophages led to an augmentation of MMP-1 expression by U937 macrophages, and high glucose further enhanced this augmentation. Similar observations were also made in the coculture of fibroblasts and human primary monocytes. We also found that interleukin 6 (IL-6) released by fibroblasts was essential for the augmentation of MMP-1 expression by U937 macrophages. Furthermore our results showed that high glucose, IL-6, and lipopolysaccharide had a synergistic effect on MMP-1 expression. Finally our study indicated that MAPK pathways and activator protein-1 transcription factor were involved in the coculture-and high glucoseaugmented MMP-1 expression. In conclusion, this study demonstrates that IL-6 derived from fibroblasts is essential for MMP-1 up-regulation by cross-talking between fibroblasts and U937 macrophages exposed to high glucose, revealing an IL-6-dependent mechanism in MMP-1 up-regulation.

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“…The chronic interaction of the host immune system with subgingival plaque containing P. gingivalis and the RgpA-Kgp complexes in the subjacent tissue is believed to be a major factor in tissue destruction in chronic periodontitis (6,21,51,83). Compared with healthy subjects, gingival tissues and gingival crevicular fluid of patients with chronic periodontitis are reported to have significantly increased amounts of proinflammatory cytokines such as interleukin-1 (IL-1), IL-6, and tumor necrosis factor alpha (TNF-␣); chemokines such as IL-8, macrophage chemoattractant protein 1 (MCP-1), and macrophage inflammatory protein 1␣ (MIP-1␣); and adhesion molecules such as intracellular adhesion molecule 1 (ICAM-1) (12,25,27,28,32,33,37,44,46,62,84,87). These cytokines and chemokines play a significant role in mediating the recruitment of a dense mononuclear infiltrate, consisting mainly of T cells and macrophages (21,22,39,55,81).…”

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Porphyromonas gingivalisRgpA-Kgp Proteinase-Adhesin Complexes Penetrate Gingival Tissue and Induce Proinflammatory Cytokines or Apoptosis in a Concentration-Dependent Manner

et al. 2009

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The RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis were observed, using immunostaining, in human gingival tissue associated with periodontitis but not in healthy tissue. The staining pattern suggested a concentration gradient from the subgingival plaque into the subjacent gingival connective tissue. Intense immunostaining was observed in areas displaying gross disturbance of tissue architecture. P. gingivalis cells and the RgpA-Kgp complexes at low concentrations were shown to stimulate secretory intercellular adhesion molecule 1, interleukin-8 (IL-8), IL-6, and macrophage chemoattractant protein secretion from cultured human epithelial (KB) and fibroblast (MRC-5) cells. However, at high concentrations a reduction in the level of these mediators was observed. In contrast, macrophage inflammatory protein 1␣ and IL-1␣ were stimulated only at high P. gingivalis cell concentrations. P. gingivalis cells and the RgpA-Kgp complexes were shown to induce apoptosis in KB and MRC-5 cells in a time-and dose-dependent manner. These data suggest that the RgpA-Kgp complexes penetrate the gingival connective tissue; at low concentrations distal from the plaque the complexes stimulate the secretion of proinflammatory mediators, while at high concentrations proximal to the plaque they induce apoptosis and attenuate the secretion of proinflammatory mediators.

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Interleukin‐1β, clinical parameters and matched cellular‐histopathologic changes of biopsied gingival tissue from periodontitis patients

Abstract: These observations suggest that IL-1beta plays a significant role in the pathogenic mechanisms of periodontal tissue destruction, and that measurement of tissue IL-1beta would be a valuable aid and useful for diagnostic markers of periodontal diseases.

Cited by 70 publications

References 46 publications

Condición periodontal de pacientes con apnea obstructiva del sueño / Periodontal Status of Patients with Obstructive Sleep Apnea

Condición periodontal de pacientes con apnea obstructiva del sueño / Periodontal Status of Patients with Obstructive Sleep Apnea

Interleukin-6 Released from Fibroblasts Is Essential for Up-regulation of Matrix Metalloproteinase-1 Expression by U937 Macrophages in Coculture

Porphyromonas gingivalisRgpA-Kgp Proteinase-Adhesin Complexes Penetrate Gingival Tissue and Induce Proinflammatory Cytokines or Apoptosis in a Concentration-Dependent Manner

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