Interleukin-1β and TNF-α systems in ovarian follicles and their roles during follicular development, oocyte maturation and ovulation

Silva, José Roberto Viana; Lima, Francisco Edilcarlos Oliveira; Souza, Ana Liza Paz; Silva, Anderson Weiny Barbalho

doi:10.1017/s0967199420000222

Cited by 26 publications

(26 citation statements)

References 105 publications

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“…Cytokines are small, soluble, and diverse pleiotropic immunoregulatory signaling proteins with a short half-life, whereas chemokines are a group of secreted proteins within the cytokine family [ 11 ]. Cytokines and chemokines create a chemotactic immune and non-immune gradient in the follicle [ 2 – 4 , 12 , 13 ]. In turn, these cytokines and chemokines govern locally follicular cell fate and homeostasis by regulating cell survival, death, proliferation, and differentiation [ 3 , 10 , 11 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Banerjee

Mishra

et al. 2022

J Ovarian Res

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Background Granulosa cells (GCs) are multilayered somatic cells within the follicle that provide physical support and microenvironment for the developing oocyte. In recent years, the role of Neuregulin-1 (NRG1), a member of the EGF-like factor family, has received considerable attention due to its neurodevelopmental and cardiac function. However, the exact physiological role of NRG1 in GC is mainly unknown. In order to confirm that NRG1 plays a regulatory role in rat GC functions, endogenous NRG1-knockdown studies were carried out in GCs using RNA interference methodology. Results Knockdown of NRG1 in GCs resulted in the enhanced expression and secretion of the cytokines and chemokines. In addition, the phosphorylation of PI3K/Akt/ERK1/2 was significantly low in GCs under these experimental conditions. Moreover, in vitro experimental studies suggest that tumor necrosis factor-α (TNFα) treatment causes the physical destruction of GCs by activating caspase-3/7 activity. In contrast, exogenous NRG1 co-treatment of GCs delayed the onset of TNFα-induced apoptosis and inhibited the activation of caspase-3/7 activity. Furthermore, current experimental studies suggest that gonadotropins promote differential expression of NRG1 and ErbB3 receptors in GCs of the antral follicle. Interestingly, NRG1 and ErbB3 were intensely co-localized in the mural and cumulus GCs and cumulus-oocyte complex of pre-ovulatory follicles in the estrus stage. Conclusions The present studies suggest that gonadotropins-dependent NRG1-signaling in GCs may require the balance of the cytokines and chemokines expression and secretion, ultimately which may be supporting the follicular maturation and oocyte competence for ovulation and preventing follicular atresia.

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Section: Introductionmentioning

confidence: 99%

Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Banerjee

Mishra

et al. 2022

J Ovarian Res

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“…As reported, IL-1β contributes to control of follicle development by enabling proliferation of granulosa cells and preventing premature differentiation. This cytokine has also been found to influence apoptosis in ovarian granulosa cells [ 57 ]. IL-1 has emerged as an important factor in the age-related depletion of ovarian reserve in mice, possibly by boosting the expression of inflammatory genes and by advancing apoptotic pathways.…”

Section: Discussionmentioning

confidence: 99%

Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF

Konstantinidou

Budani

Sarra

et al. 2021

IJMS

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The female reproductive system represents a sensitive target of the harmful effects of cigarette smoke, with folliculogenesis as one of the ovarian processes most affected by this exposure. The aim of this study was to analyze the impact of tobacco smoking on expression of oxidative stress-related genes in cumulus cells (CCs) from smoking and non-smoking women undergoing IVF techniques. Real time PCR technology was used to analyze the gene expression profile of 88 oxidative stress genes enclosed in a 96-well plate array. Statistical significance was assessed by one-way ANOVA. The biological functions and networks/pathways of modulated genes were evidenced by ingenuity pathway analysis software. Promoter methylation analysis was performed by pyrosequencing. Our results showed a down-regulation of 24 genes and an up-regulation of 2 genes (IL6 and SOD2, respectively) involved in defense against oxidative damage, cell cycle regulation, as well as inflammation in CCs from smoking women. IL-6 lower promoter methylation was found in CCs of the smokers group. In conclusion, the disclosed overall downregulation suggests an oxidant-antioxidant imbalance in CCs triggered by cigarette smoking exposure. This evidence adds a piece to the puzzle of the molecular basis of female reproduction and could help underlay the importance of antioxidant treatments for smoking women undergoing IVF protocols.

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“…Most of follicles undergo degradation and atresia through apoptosis (26). The basic conditions required for follicular development and maturation depend on the proliferation and differentiation states of granulosa cells in the follicles, where apoptosis of granulosa cells is a prerequisite for follicular atresia (27). This directly affects the quantity and quality of follicles.…”

Section: Discussionmentioning

confidence: 99%

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

Zhang

et al. 2021

Exp Ther Med

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Pueratin (Pue) is an extract from Pueraria lobata, and exhibits therapeutic effects for the treatment of inflammation. However, the beneficial effects and mechanisms underlying Pue in the treatment of diminished ovarian reserve (DOR) remains to be fully elucidated. The aim of the present study was to investigate the effect of Pue on Bcl-2 and Bax protein expression in rats with DOR, associated with infertility within clinical practice, induced by 4-vinylcyclohexene diepoxide (VCD). A model of DOR was established in female Sprague Dawley rats by an intraperitoneal injection of 80 mg/kg VCD daily for 45 days. From day 1, the Sprague Dawley rats were orally administered with drugs daily for 45 days. They were divided into normal, model, Pue-low dose (L), Pue-medium dose (M) and Pue-high dose (H) groups (50, 100 and 300 mg/kg Pue, respectively). Follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E 2 ) levels were subsequently detected using ELISA. H&E staining and TUNEL staining were used to evaluate histopathological changes and apoptosis levels in the ovary, respectively. Bcl-2 and Bax protein expression levels in rat ovaries were evaluated using immunohistochemistry and western blotting. Compared with those in the model group, FSH and LH levels in the Pue-L, -M and -H groups were significantly decreased, whilst E 2 levels were significantly increased (P<0.05). After intragastric administration, the volume of the ovaries and uteri of rats in the Pue groups was increased compared with the model group, and the numbers of primordial follicles and primary follicles were also increased. The number of apoptotic cells and the expression of Bax were significantly reduced in a dose-dependent manner (P<0.05), compared with the model group. In addition, Bcl-2 protein expression and the Bcl-2/Bax ratio were found to be significantly increased in the Pue-treated groups in a dose-dependent manner (P<0.05), compared with the model group. In conclusion, Pue treatment improved ovarian function by regulating hormone balance in addition to Bcl-2 and Bax expression.

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Interleukin-1β and TNF-α systems in ovarian follicles and their roles during follicular development, oocyte maturation and ovulation

Cited by 26 publications

References 105 publications

Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell

Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF

Pueratin improves diminished ovarian reserve by inhibiting apoptosis

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