Interleukin‑1α and ‑1β assessment in the gingival crevicular fluid of periodontal patients with chronic hepatitis C

Șurlin, Petra; Gheorghe, Dorin Nicolae; Popescu, Dora Maria; Mârțu, Maria-Alexandra; Solomon, Sorina Mihaela; Roman, Alexandra; Lazăr, Luminița; Stratul, Ştefan‐Ioan; Rusu, Darian; Foia, Liliana; Boldeanu, Mihail Virgil; Boldeanu, Lidia; Danilescu, Monica; Rogoveanu, Ion

doi:10.3892/etm.2020.8906

Cited by 10 publications

(13 citation statements)

References 36 publications

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“…In essence, both CHC and P generate a chronic inflammatory reaction, such a pathologic event being driven by proinflammatory mediators that control its extent and intensity [15,16]. Our previously published study, focusing on the gingival crevicular fluid (GCF) assessment of interleukin-1alpha's and interleukin-1beta's involvement in the pathogenic process of periodontitis patients with chronic hepatitis C, highlighted a significantly worsened periodontal status and increased levels of these cytokines in patients with both diseases, as compared to those of nonhepatitis C patients suffering from periodontitis [17]. This suggests the negative impact that hepatic pathology may have on local periodontal inflammation.…”

Section: Introductionmentioning

confidence: 99%

NLRP3 Inflammasome Expression in Gingival Crevicular Fluid of Patients with Periodontitis and Chronic Hepatitis C

Șurlin

Lazăr

Şincar

et al. 2021

Mediators of Inflammation

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The study is aimed at assessing the impact that periodontal disease and chronic hepatitis C could have on gingival crevicular fluid levels of the NLRP3 inflammasome, caspase-1 (CASP-1), and interleukin-18 (IL-18) and at evaluating whether the increased local inflammatory reaction with clinical periodontal consequences is correlated to their upregulation. Patients were divided into four groups, according to their periodontal status and previously diagnosed hepatitis C, as follows: (i) CHC group, chronic hepatitis C patients; (ii) P group, periodontal disease patients, systemically healthy; (iii) CHC + P group, patients suffering from both conditions; and (iv) H group, systemically and periodontally healthy controls. Gingival crevicular samples were collected for quantitative analysis of the NLRP3 inflammasome, CASP-1, and IL-18. CHC + P patients expressed the worse periodontal status and the highest NLRP3, CASP-1, and IL-18 levels, the difference being statistically significant ( p < 0.05 ). The P group patients also expressed significantly more elevated NLRP3, CASP-1, and IL-18 levels, as compared to nonperiodontal patients (CHC and H groups). Chronic hepatitis C and periodontal disease could have a significant influence on the upregulation of NLRP3 inflammasome and its components, possibly contributing to an increased local inflammatory reaction and clinical periodontal consequences.

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Section: Introductionmentioning

confidence: 99%

NLRP3 Inflammasome Expression in Gingival Crevicular Fluid of Patients with Periodontitis and Chronic Hepatitis C

Șurlin

Lazăr

Şincar

et al. 2021

Mediators of Inflammation

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“…Specifically, the elevated presence of IL-1 family proteins, including both the cytokines and the receptors, has been found to participate in the oral host-responses to microbial infection, as well as in extracellular matrix catabolism, bone resorption, and periodontal tissue remodeling [ 19 , 34 – 36 ]. In the hypothesized gene-environment interaction model for the etiology of CP, it is believed that genetic variations affecting the production and/or function of the interleukins may cause altered immune responses following periodontal infection and thereby lead to different levels of risk of developing this disease [ 21 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…They are well-known key regulators in both innate and adaptive immunity and have been highlighted in a variety of infectious and autoimmune diseases, as well as in systemic inflammatory responses [ 20 ]. IL-1 α is generally recognized as a proinflammatory mediator facilitating the pathogenesis of multiple infectious diseases [ 21 ], and genetic variations in L-1 α are found to be associated with the onset and progression of CP. Specifically, the SNP rs1800587 (also known as IL-1 α (−889 C/T)) is significantly associated with CP but not AgP [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Association of Interleukin-1α Functional Polymorphism with Risk of Chronic Periodontitis in Han Chinese Population

2021

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Chronic periodontitis (CP) is a common inflammatory illness affecting a large proportion of humans. Genetic factors are thought to play important roles in its onset and development. A functional polymorphism (rs1800587) in the promoter of the interleukin-1α gene (−889 C/T) has been found to confer risk of CP primarily in Europeans, but the association between this variant and CP in the Chinese population remains less conclusive. In the current study, we aimed to investigate the association between rs1800587 and CP in case-control samples of Han Chinese origin. A total of 1,777 study subjects, including 884 CP patients and 893 healthy controls, were collected. Genotyping of rs1800587 was performed using the SNAPSHOT method, and statistical analyses were conducted to evaluate the association between rs1800587 and CP. In our sample, rs1800587 was significantly associated with the onset of CP (additive model, T-allele vs. C-allele, p = 0.00359, odds ratio = 1.446, 95% confidence intervals (CIs) = 1.127–1.855; dominant model, (TT + TC) vs. CC, p = 0.00250, odds ratio = 1.502, 95% CIs = 1.152–1.957; overdominant model, TC vs. (TT + CC), p = 0.00264, odds ratio = 1.508, 95% CIs = 1.152–1.976). The T-allele and [TC] genotypes of rs1800587 were significantly overrepresented in CP patients compared with controls. Our data suggest that rs1800587 of IL-1α is significantly associated with the risk of CP in Han Chinese subjects, further confirming its possible involvement in the disease.

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“…The gingival crevicular fluid (GCF) is a relevant and easy-to-sample biologic product for the detection of pro-inflammatory mediators, that have been shown to closely followthe severity and intensity of the local periodontal inflammatory reaction, in terms of their quantitative analysis [17]. The study of pro-inflammatory cytokines (interleukin-1alpha and -1beta) in GCF samples has revealed that their levels were significantly up-regulated in CHC periodontitis patients, compared to non-CHC ones [18].…”

Section: Introductionmentioning

confidence: 99%

Non-Surgical Periodontal Therapy Could Improve the Periodontal Inflammatory Status in Patients with Periodontitis and Chronic Hepatitis C

Gheorghe

Popescu

Sălan

et al. 2021

JCM

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Non-surgical periodontal therapy (NSPT) is the first essential step for the management of any periodontitis patient. This study aims to evaluate the impact of NSPT on pro-inflammatory mediators’ regulation and on clinical parameters in periodontitis patients who suffer from chronic hepatitis C. At baseline, selected patients were clinically evaluated for their periodontal status. A subsequent quantitative assessment of C-reactive protein and pentraxin-3 in samples of gingival fluid was performed by Enzyme-Linked Immunosorbent Assay (ELISA). Afterwards, NSPT was performed. Three months after NSPT, the clinical and ELISA assessments were repeated. The results show an improvement of the clinical parameters in periodontitis patients at the three-month recall. In chronic hepatitis C patients with periodontitis, the gingival fluid levels of pro-inflammatory markers reduced significantly. The targeted markers also expressed significant correlations with the clinical parameters used for the assessment of periodontitis’ severity. The results suggest that, while chronic hepatitis C patients exhibited a more negative periodontal status at baseline as compared to non-hepatitis ones, NSPT is effective in decreasing the local periodontal inflammatory reaction and in proving the periodontal status of this type of patients. Given the limitation of the study, periodontal screening and NSPT should be included in the integrated therapeutical approach of chronic hepatitis C patients, for its impact on the local inflammatory response.

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Interleukin‑1α and ‑1β assessment in the gingival crevicular fluid of periodontal patients with chronic hepatitis C

Cited by 10 publications

References 36 publications

NLRP3 Inflammasome Expression in Gingival Crevicular Fluid of Patients with Periodontitis and Chronic Hepatitis C

NLRP3 Inflammasome Expression in Gingival Crevicular Fluid of Patients with Periodontitis and Chronic Hepatitis C

Association of Interleukin-1α Functional Polymorphism with Risk of Chronic Periodontitis in Han Chinese Population

Non-Surgical Periodontal Therapy Could Improve the Periodontal Inflammatory Status in Patients with Periodontitis and Chronic Hepatitis C

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