Interleukin-18 enhances the production of interferon-gamma (IFN-γ) by allergen-specific and unspecific stimulated cord blood mononuclear cells

Ngoumou, Gonza; Schaefer, Doerte; Mattes, Joërg; Kopp, Matthias

doi:10.1016/j.cyto.2003.11.013

Cited by 7 publications

(3 citation statements)

References 35 publications

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“…IL-18, originally called IFN- γ inducing factor , is known to enhance the secretion of IFN-γ from NK cells and CD4+ T helper 1 lymphocytes (49). It has been shown that in combination with IL-15, IL-12, or with allergens IL-18 enhances, or synergistically induces the production of IFN-γ in cord blood mononuclear cells (50) or in NK cells (51). IFN-γ in turn may prime macrophages to pro-inflammatory M1 phenotype when combined with bacterial LPS (28).…”

Section: Discussionmentioning

confidence: 99%

High Levels of IL-18 and IFN-γ in Chronically Inflamed Tissue in Chronic Granulomatous Disease

et al. 2019

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Background: Chronic granulomatous disease (CGD) is caused by a malfunctioning nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex in phagocytes, leading to impaired bacterial and fungal killing and hyperinflammation.Objective: To characterize macrophage subsets and cytokine/chemokine signaling loops involved in CGD tissue hyperinflammation.Methods: Cytokine/chemokine production and surface marker expression were analyzed in inflamed tissue of four CGD patients and compared to cytokine/chemokine released by CGD macrophages upon priming to different macrophage subpopulations. Furthermore, the re-priming capacity of CGD pro-inflammatory M1 to M2a anti-inflammatory macrophages was evaluated.Results: In human CGD inflammatory tissue, IL-18 and IFN-γ were detected in significant quantity. Immunofluorescence analysis identified macrophages as one source of IL-18 in inflamed tissue. In vitro, CGD macrophages could be primed and re-primed into all inflammatory/anti-inflammatory macrophage subpopulations. IL-18 was also released by M1 CGD and control macrophages.Conclusion: CGD pro-inflammatory M1 macrophages remain M1 primed in vivo. As CGD M1 macrophages can be re-primed to anti-inflammatory M2a phenotype in vitro, macrophages are kept in M1 state in vivo by a persistent pro-inflammatory environment. Our results suggest a paracrine signaling loop between M1 macrophage derived IL-18 and non-macrophage derived IFN-γ maintaining macrophage pro-inflammatory activity in CGD tissue.

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Section: Discussionmentioning

confidence: 99%

High Levels of IL-18 and IFN-γ in Chronically Inflamed Tissue in Chronic Granulomatous Disease

et al. 2019

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“…Others have reported a predominant increase in iFn-γ concentrations in children with bronchiolitis (5). this increase in iFn-γ concentrations appears to be secondary to the increase in il-18 concentrations, as this interleukin is one of the most powerful agents stimulating the production and release of iFn-γ (20). the relationships between infection and il-15 or nK cell activity are similar.…”

Section: Discussionmentioning

confidence: 54%

“…IL-18 is a pro-inflammatory cytokine with pleiotropic properties and plays a crucial role in the maintenance of th1-cell response. this cytokine activates nK cells, leads to eosinophilia and increases histamine concentrations (17)(18)(19)(20). there are various reports indicating that, during viral infection, il-15, il-18 and iFn-γ concentrations are elevated (21).…”

Section: Introductionmentioning

confidence: 99%

Concentrations of IL-15, IL-18, IFN-γ and activity of CD4+, CD8+ and NK cells at admission in children with viral bronchiolitis

Grześk

Kołtan

Dębski

et al. 2010

Experimental and Therapeutic Medicine

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Abstract. the pathogenesis of viral bronchiolitis is poorly understood. the aim of this study was to analyze interleukin (il)-15, il-18 and interferon (iFn)-γ concentrations and the activity of nK cells and cd4 + and cd8 + lymphocytes in 23 children not older than 30 months of age with acute viral bronchiolitis using blood samples drawn within the first 24 h of their hospital admission, in comparison to a healthy group. in children with bronchiolitis, the mean concentrations of il-15, il-18 and iFn-γ were 9.39±11.55, 884.03±645.44 and 17.92±27.14 pg/ml, respectively, and were significantly higher than those in the control group [2.34±0.61 pg/ml (p<0.05), 248.69±98.73 pg/ml (p<0.001) and 2.75±1.72 pg/ml (p<0.005), respectively]. in the bronchiolitis group, mean z-scores were -1.15±1.9 for cd4 + cells and -0.9±1.23 for cd8 + cells; these scores were significantly lower than those of the general polish population (p<0.001 and <0.01, respectively). however, the mean z-score of the ratio of cd4 + /cd8 + and the nK cell count in children with bronchiolitis did not differ significantly from those of the controls. in conclusion, cytokines such as il-15, il-18 and iFn-γ play a role in the pathogenesis of bronchiolitis in children. IntroductionBronchiolitis in children is a serious self-limiting disease (mortality rate <1%) of respiratory tract infections. however, in high-risk groups such as children with bronchopulmonary dysplasia, congenital heart disease or cystic fibrosis, mortality increases to 5-10%. the leading cause of bronchiolitis are viral infections, with the most common agent being respiratory syncytial virus infection (rSV) (60-80% of cases) (1-4). during rSV infection, the cytokine cascade is activated, leading to the activation of th1 and th2 lymphocytes. thus, an increase in the concentrations of cytokines such as interleukin (il)-2, -6, -10, -12, -13 and a decrease in interferon (iFn)-γ and il-4 concentrations are observed (5-12). during non-rSV viral infections, the th1 type response of the immunologic system with an increase in the iFn-γ concentration is commonly observed (5,8,13,14).il-15 and il-18 are relatively newly discovered cytokines that are produced principally by macrophages during immune response. il-15 has multiple biological properties, including the induction of the production of other cytokines and the inhibition of T-cell apoptosis (15,16). IL-18 is a pro-inflammatory cytokine with pleiotropic properties and plays a crucial role in the maintenance of th1-cell response. this cytokine activates nK cells, leads to eosinophilia and increases histamine concentrations (17)(18)(19)(20). there are various reports indicating that, during viral infection, il-15, il-18 and iFn-γ concentrations are elevated (21). a secondary increase in nK cell activity has also been reported (22). however, the role of il-15 and il-18 in viral bronchiolitis in children remains unknown. the aim of this study was to analyze il-15, il-18 and iFn-γ concentrations and the activity of nK cells as well as cd4 + and cd8 +...

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Clinical value of serum interleukin-18 and nitric oxide activities in patients with prostate cancer

Nong

Zhang

et al. 2011

Chin. -Ger. J. Clin. Oncol.

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Objective: The aim of the study was to estimate the clinical value of serum interleukin-18 (IL-18) and nitric oxide (NO) activities in patients with prostate cancer. Methods: The 50 patients with prostate cancer and 25 control subjects were measured in serum IL-18 by enzyme-linked immunosorbent assay (ELISA) and nitrate + nitrite level by an index of NO generation. Results: Serum IL-18 and nitrate + nitrite levels were significantly higher in patients with prostate cancer when compared to the control subjects (P < 0.05). Serum IL-18 level was significantly higher in patients with stages B, C and D when compared to patients with stage A (P < 0.05). Serum IL-18 level was significantly higher in the metastatic patients compared with the nonmetastatic patients (P < 0.01). There was no difference in serum nitrate and nitrite level between metastatic and nonmetastatic patients (P > 0.05). The serum IL-18 and nitrate and nitrite levels decreased after patients underwent surgical resection. Conclusion: Serum IL-18 level may be a useful marker to predict prognosis of patients with prostate cancer after surgery. Long-term follow-up is required to clarify this hypothesis.

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Interleukin-18 enhances the production of interferon-gamma (IFN-γ) by allergen-specific and unspecific stimulated cord blood mononuclear cells

Cited by 7 publications

References 35 publications

High Levels of IL-18 and IFN-γ in Chronically Inflamed Tissue in Chronic Granulomatous Disease

High Levels of IL-18 and IFN-γ in Chronically Inflamed Tissue in Chronic Granulomatous Disease

Concentrations of IL-15, IL-18, IFN-γ and activity of CD4+, CD8+ and NK cells at admission in children with viral bronchiolitis

Clinical value of serum interleukin-18 and nitric oxide activities in patients with prostate cancer

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