Interleukin-17A Differentially Induces Inflammatory and Metabolic Gene Expression in the Adipose Tissues of Lean and Obese Mice

Abstract

The functions of interleukin-17A (IL-17A) in adipose tissues and adipocytes have not been well understood. In the present study, male mice were fed with a regular diet (n = 6, lean mice) or a high-fat diet (n = 6, obese mice) for 30 weeks. Subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) were analyzed for IL-17A levels. SAT and VAT were treated with IL-17A and analyzed for inflammatory and metabolic gene expression. Mouse 3T3-L1 pre-adipocytes were differentiated into adipocytes, followed with IL-17A treatment and analysis for inflammatory and metabolic gene expression. We found that IL-17A levels were higher in obese SAT than lean SAT; the basal expression of inflammatory and metabolic genes was different between SAT and VAT and between lean and obese adipose tissues. IL-17A differentially induced expression of inflammatory and metabolic genes, such as tumor necrosis factor α, Il-6, Il-1β, leptin, and glucose transporter 4, in adipose tissues of lean and obese mice. IL-17A also differentially induced expression of inflammatory and metabolic genes in pre-adipocytes and adipocytes, and IL-17A selectively activated signaling pathways in adipose tissues and adipocytes. These findings suggest that IL-17A differentially induces inflammatory and metabolic gene expression in the adipose tissues of lean and obese mice.

Gut dysbiosis and impairment of immune system homeostasis in perinatally-exposed mice to Bisphenol A precede obese phenotype development

“…In our study, the M1 macrophages infiltration is associated with IL-17 overexpression in gWAT. This finding is concordant with studies showing tight correlation between IL-17 inflammation in WAT and obesity 44 , 45 . All these results confirm the obesogenic effect of perinatal exposure to BPA in offspring mice, with close characteristics to human obese phenotype, hence validate our in vivo model to study the chronological development of immune and gut microbiota imbalance associated with this phenotype.…”

Salt Induces Adipogenesis/Lipogenesis and Inflammatory Adipocytokines Secretion in Adipocytes

“…The secretion of IL-17A was found to be more active in salt-loading adipocytes. Qu et al has shown that increased expression of IL-17A also increases the expression of metabolic genes [45]. It has also been reported that paracrine agents released by high salt cause changes in the lipogenesis and lipolysis of adipose tissues by recruiting macrophages and T helper cells [46].…”

Psoriasis and Cardiovascular Comorbidities: Focusing on Severe Vascular Events, Cardiovascular Risk Factors and Implications for Treatment

“…The cytokine profiles of psoriasis skin lesions and atherosclerosis vascular lesions are very similar, showing increased number of Th1 and Th17 lymphocytes [ 186 , 196 , 197 ]. The Th1 and Th17 cytokine pathways have been shown to be involved in the pathogenesis of both psoriasis and atherosclerosis [ 197 , 198 , 199 , 200 , 201 , 202 ]. Similar to psoriasis, patients with ischemic heart disease have increased levels of Th17-related cytokines (IL-17, IL-6 and IL-8) in their peripheral blood [ 203 ].…”