Interleukin-12 (IL-12)/IL-10 Ratio as a Marker of Disease Severity in Crimean-Congo Hemorrhagic Fever

Watson, Dionysios C.; Σαργιάνου, Μαρία; Panos, George

doi:10.1128/cvi.00030-12

Cited by 16 publications

(15 citation statements)

References 8 publications

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“…While IL‐12 activates the Th1 immune response, the IL‐10 regulates negatively this process [O'Garra and Murphy, ]; this opposing relationship between pro‐inflammatory and anti‐inflammatory cytokines is the basis for assessment of the immune status in various diseases, including CCHF [Watson et al, ]. The mean IL‐12/IL‐10 ratio in the present study was low (0.09) in the fatal cases, while it was >1 in survivors.…”

Section: Discussionmentioning

confidence: 58%

Cytokines as biomarkers of Crimean‐Congo hemorrhagic fever

Papa

Tsergouli

Çağlayık³

et al. 2015

Journal of Medical Virology

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Crimean-Congo hemorrhagic fever (CCHF) is a potentially severe disease caused by CCHF virus. As in other viral hemorrhagic fevers, it is considered that the course and outcome of the disease depend on the viral load and the balance among the immune response mediators, and that a fatal outcome is the result of a "cytokine storm." The level of 27 cytokines was measured in serum samples taken from 29 patients during the acute phase of the disease. Two cases were fatal. Among survivors, significant differences between severe and non-severe cases were observed in the levels of IP-10, and MCP-1, while the levels of IL-1b, IL-5, IL-6, IL-8, IL-9, IL-10, IL-15, IP-10, MCP-1, TNF-α, and RANTES differed significantly between fatal and non-fatal cases (P < 0.05). RANTES was negatively correlated with the outcome of the disease. A striking similarity with the cytokine patterns seen in Ebola virus disease was observed. A weak Th1 immune response was seen. The viral load was positively correlated with IL-10, IP-10, and MCP-1 levels, and negatively correlated with the ratio IL-12/IL-10. Especially IP-10 and MCP-1 were significantly associated with the viral load, the severity and outcome of the disease, and they could act as biomarkers and, probably, as potential targets for treatment strategies design.

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Section: Discussionmentioning

confidence: 58%

Cytokines as biomarkers of Crimean‐Congo hemorrhagic fever

Papa

Tsergouli

Çağlayık³

et al. 2015

Journal of Medical Virology

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“…DCs utilize IL‐12p40 to mediate differentiation of naïve T lymphocytes into a Th1 or Th17 phenotype. IL‐10 opposes this process, and the ratio of IL‐12 : IL‐10 is often used to assess inflammatory status . Interestingly, even though the effects of α BC on IL‐10, H 2 O 2 and MMP8 were small, treatment of neutrophils with the crystallin was capable of reducing secretion of IL‐12p40 by co‐cultured DCs in a cell‐ratio‐dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Murine neutrophils treated with alphaB‐crystallin reduce IL‐12p40 production by dendritic cells

2018

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Neutrophils are essential in the fight against invading pathogens. They utilize antimicrobial effector mechanisms, such as phagocytosis, release of proteases and other antimicrobial products, robust oxidative bursts and neutrophil extracellular traps to combat infections. Neutrophils also modulate immune responses through the production of eicosanoids, cytokines and chemokines, as well as via direct communication with other immune cells. This system of high-intensity offense against pathogens is exquisitely balanced through regulation to limit damage to host tissue. Unfortunately, the control of neutrophils is not failproof. In cases of sterile injury, autoimmunity and even during an infection, neutrophils can cause tissue destruction and become detrimental to the host. For that reason, there is a need to find means to regulate the aberrant activation of these cells. We found that alphaB-crystallin (αBC), a heat-shock protein known to have anti-inflammatory abilities, affects certain properties of mouse neutrophils that subsequently influence the pro-inflammatory state of antigen-presenting cells (APCs). More specifically, αBC mediated small but significant increases in the levels of IL-10 and matrix metalloproteinase 8, and altered hydrogen peroxide secretion by stimulated neutrophils. Further, the heat-shock protein influenced the communication between neutrophils and dendritic cells by decreasing the production of pro-inflammatory cytokines, specifically IL-12p40, by the APCs. αBC could thus contribute to dampening neutrophil inflammatory responses by impacting the effect of neutrophils on other immune cells.

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“…Both IL-12 and IL-10 are key cytokines that play major roles in regulating inflammatory responses (54,(101)(102)(103). The IL-12 p40/p35 heterodimer is essential for the initiation of an effective immune response, although IL-10 protects the host from excessive inflammation (54,101).…”

Section: Discussionmentioning

confidence: 99%

Medroxyprogesterone Acetate Differentially Regulates Interleukin (IL)-12 and IL-10 in a Human Ectocervical Epithelial Cell Line in a Glucocorticoid Receptor (GR)-dependent Manner

Toit

Hapgood

Africander

2014

Journal of Biological Chemistry

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Background:Little is known about the mechanism of action of MPA in the female genital tract. Results: GR mediates MPA-induced up-regulation of IL-12 and down-regulation of IL-10 mRNA and protein levels. Conclusion: MPA favors a pro-inflammatory milieu in ectocervical epithelial cells. Significance: MPA used in hormonal therapy may modulate inflammation in the ectocervical environment via this genomic mechanism.

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Interleukin-12 (IL-12)/IL-10 Ratio as a Marker of Disease Severity in Crimean-Congo Hemorrhagic Fever

Cited by 16 publications

References 8 publications

Cytokines as biomarkers of Crimean‐Congo hemorrhagic fever

Cytokines as biomarkers of Crimean‐Congo hemorrhagic fever

Murine neutrophils treated with alphaB‐crystallin reduce IL‐12p40 production by dendritic cells

Medroxyprogesterone Acetate Differentially Regulates Interleukin (IL)-12 and IL-10 in a Human Ectocervical Epithelial Cell Line in a Glucocorticoid Receptor (GR)-dependent Manner

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