Interleukin-11 increases invasiveness of JEG-3 choriocarcinoma cells by modulating STAT3 expression

Suman, Pankaj; Poehlmann, Tobias G.; Golla, Jaya Prakash; Markert, Udo R.; Gupta, Seema

doi:10.1016/j.jri.2009.07.002

Cited by 37 publications

(27 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is substantial evidence that STAT3-regulating cytokines including LIF and IL6 have key roles as regulators of trophoblast differentiation and invasive function [34]–[36]. However, a direct role for STAT3 in trophoblasts is unlikely to explain the effects seen herein, as conceptus-derived tissues are hemizygous for STAT3 deficiency by virtue of their wild-type paternal genome, and STAT3 heterozygous embryos have no developmental defects.…”

Section: Discussionmentioning

confidence: 87%

Identification of Sites of STAT3 Action in the Female Reproductive Tract through Conditional Gene Deletion

et al. 2014

View full text Add to dashboard Cite

The STAT3 transcription factor is a pleiotropic transducer of signalling by hormones, growth factors and cytokines that has been identified in the female reproductive tract from oocytes and granulosa cells of the ovary to uterine epithelial and stromal cells. In the present study we used transgenic models to investigate the importance of STAT3 for reproductive performance in these different tissues. The Cre-LoxP system was used to delete STAT3 in oocytes by crossing Stat3fl/fl with Zp3-cre+ mice, or in ovarian granulosa cells and uterine stroma by crossing with Amhr2-Cre+ mice. Surprisingly, deletion of STAT3 in oocytes had no effect on fertility indicating that the abundance of STAT3 protein in maturing oocytes and fertilized zygotes is not essential to these developmental stages. In Stat3fl/fl;Amhr2-cre+ females impaired fertility was observed through significantly fewer litters and smaller litter size. Ovulation rate, oocyte fertilization and development to blastocyst were unaffected in this line; however, poor recombination efficiency in granulosa cells had yielded no net change in STAT3 protein abundance. In contrast, uteri from these mice showed STAT3 protein depletion selectively from the stomal compartment. A significant reduction in number of viable fetuses on gestational day 18, increased fetal resorptions and disrupted placental morphology were evident causes of the reduced fertility. In conclusion, this study defines an important role for STAT3 in uterine stromal cells during embryo implantation and the development of a functional placenta.

show abstract

Section: Discussionmentioning

confidence: 87%

Identification of Sites of STAT3 Action in the Female Reproductive Tract through Conditional Gene Deletion

et al. 2014

View full text Add to dashboard Cite

show abstract

“…JEG-3 (DZMO, Braunschweig, Germany) and ACH-3P cells (a gift from Dr. Gernot Desoye, Medical University of Graz, Graz, Austria) were cultured as described previously (14). Both the cell lines have been extensively characterized for their resemblance to primary trophoblast cells (15,16).…”

Section: Trophoblast Cell Linesmentioning

confidence: 99%

“…In vitro Matrigel invasion assay was performed as described previously (14). The growth factor-reduced Matrigel matrix (BD Biosciences, Bedford, MA) was prepared onto a 8-mm-pore size transwell insert (Greiner Bio-One, Frickenhausen, Germany).…”

Section: In Vitro Invasion Assaysmentioning

confidence: 99%

Decidualized endometrial stromal cell derived factors promote trophoblast invasion

Godbole¹,

Suman²,

Gupta³

et al. 2011

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

“…IL-11 is a member of the IL-6 superfamily and has been shown to stimulate first trimester CTB migration (Paiva et al 2007) but inhibit HTR-8/ SVneo cell line invasion (Paiva et al 2009). It has also been shown to stimulate JEG3 invasion (Suman et al 2009). Transforming growth fac- tor (TGF)-b1 has been shown to inhibit migration and invasion of first trimester CTB or EVT isolates, or from explants (Graham et al 1992(Graham et al , 1993(Graham et al , 1994Lash et al 2005) as well as HTR-8/SVneo and NCP cells (Graham 1997;Zhao et al 2006).…”

Section: Cytokine and Growth Factor Regulation Of Trophoblast Invasionmentioning

confidence: 99%

Molecular Cross-Talk at the Feto–Maternal Interface

Lash

2015

Cold Spring Harb Perspect Med

View full text Add to dashboard Cite

Molecular cross-talk at the feto-maternal interface occurs between many different cell types, including uterine leukocytes, extravillous trophoblast cells, and uterine spiral arteries, is essential for the establishment and maintenance of pregnancy. This review concentrates on human pregnancy and examines three main areas in which cross-talk occurs; immune tolerance, regulation of extravillous trophoblast invasion, and remodeling of the uterine spiral arteries.

show abstract

Interleukin-11 increases invasiveness of JEG-3 choriocarcinoma cells by modulating STAT3 expression

Cited by 37 publications

References 33 publications

Identification of Sites of STAT3 Action in the Female Reproductive Tract through Conditional Gene Deletion

Identification of Sites of STAT3 Action in the Female Reproductive Tract through Conditional Gene Deletion

Decidualized endometrial stromal cell derived factors promote trophoblast invasion

Molecular Cross-Talk at the Feto–Maternal Interface

Contact Info

Product

Resources

About