Interleukin-10 promoter polymorphism predicts initial response of chronic hepatitis B to interferon alfa

Abstract

In order to examine whether variation in interleukin-10 promoter polymorphism would predict the likelihood of sustain response of chronic hepatitis B to treatment with interferon alfa (IFN-α), the inheritance of 3 biallelic polymorphisms in the IL-10 gene promoter in patients with 52 chronic hepatitis B were determined by polymerase chain reaction (PCR)-bared techniques, restriction enzyme digestion or direct sequencing. The relationship to the outcome of antiviral therapy for chronic HBV infection was studied in 24 patients who had a virologically sustained response(SR) and in 28 non-responder(NR) to interferon alfa-2b and several IL-10 variants were more frequent among SR compared with NR. Carriage of the -592A allele, -592A/A genotype and -1082/-1819/-592 ATA haplotype was associated with SR. Our findings indicate that heterogeneity in the promoter region of the IL-10 gene has a role in determining the initial response of chronic hepatitis B to IFN-α therapy.

Association of interleukin-10 polymorphisms with chronic hepatitis C virus infection in a case-control study and its effect on the response to combined pegylated interferon/ribavirin therapy

“…Similarly, the T allele frequency (37%) of IL-10 -819C/T (MaeIII) was similar to that reported previously in a Caucasian population (29-36%) [20,21,23], while this frequency was shown to be relatively higher (70-81%) in an Oriental population [2,22]. Our data further reveal that the frequency of the A allele (36%) of IL-10 -592A/C (RsaI) is similar (36%) to the Caucasian population (31-33·5%) [17,20,24], while this frequency is relatively higher (52-77%) in the Oriental population [2,22]. The significant increase in frequency of the GG genotype of IL-10 -1082A/G (MnII) in chronic HCV patients compared to controls suggests that the polymorphism in IL-10 -1082A/G (MnII) may modify the susceptibility of an individual to chronic HCV infection.…”

“…* P < 0·05 is considered statistically significant. Caucasian population (37-42%) [17,20,21], while the Oriental population carry a much lower frequency (3·5%) of the G allele [2,22]. Similarly, the T allele frequency (37%) of IL-10 -819C/T (MaeIII) was similar to that reported previously in a Caucasian population (29-36%) [20,21,23], while this frequency was shown to be relatively higher (70-81%) in an Oriental population [2,22].…”

“…Caucasian population (37-42%) [17,20,21], while the Oriental population carry a much lower frequency (3·5%) of the G allele [2,22]. Similarly, the T allele frequency (37%) of IL-10 -819C/T (MaeIII) was similar to that reported previously in a Caucasian population (29-36%) [20,21,23], while this frequency was shown to be relatively higher (70-81%) in an Oriental population [2,22]. Our data further reveal that the frequency of the A allele (36%) of IL-10 -592A/C (RsaI) is similar (36%) to the Caucasian population (31-33·5%) [17,20,24], while this frequency is relatively higher (52-77%) in the Oriental population [2,22].…”

Association between the <b><i>IFNA1 </i></b>(-2C&#x2192;T) Polymorphism and Increased <b><i>IFNAR1 </i></b>Gene Expression Levels in Chronic Hepatitis B Infection

“…Cytokine production-inducing cell-mediated antiviral immune responses play a crucial role in the control of viral infection. Genetic variants of cytokines involving IFN-γ and IL-10 genes have been associated with the outcome of HBV infection [8,9,10]. Therefore, regulatory regions of SNPs and cytokine-related gene expression are important genetic predictors of disease susceptibility or clinical outcome of chronic HBV infection.…”

“…The cytokine production-induced cell-mediated antiviral immune response plays a crucial role in the control of viral infection. Genetic variants of cytokines involving interferon (IFN)-γ and interleukin (IL)-10 genes, for example, have been associated with the outcome of HBV infection [8,9,10]. …”