Interleukin-10 inhibits initial reverse transcription of human immunodeficiency virus type 1 and mediates a virostatic latent state in primary blood-derived human macrophages in vitro

Montaner, Luis J.; Griffin, Philip; Gordon, Siamon

doi:10.1099/0022-1317-75-12-3393

Cited by 53 publications

(32 citation statements)

References 32 publications

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“…The TNF-α has been shown, along with IL-8, to be involved in viral transplacental transmission, 33 and, perhaps most importantly, HIV replication during P. falciparum infection has been reported to be mediated by TNF-alpha; 34 The significantly lower plasma level of TNF-α found in our study at delivery compared with inclusion may reflect 1) the direct influence of IL-10 on this cytokine 24,35 or 2) the markedly lower overall prevalence of plasmodial infection, or 3) the decreased immune activation associated with institution of ART.…”

Section: Discussioncontrasting

confidence: 43%

Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Ibitokou¹,

Denoeud-Ndam²,

Ezinmègnon³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. We investigated the circulating plasma levels of Th1-(Interleukin-2 [IL-2], tumor necrosis factor-α [TNF-α], interferon-gamma [IFN-γ]) and Th2-type (IL-4, IL-5, IL-10) cytokines in human immunodeficiency virus (HIV)-infected pregnant women living in a malaria-endemic area. We analyzed samples from 200 pregnant women included in the prevention of pregnancy-associated malaria in HIV-infected women: cotrimoxazole prophylaxis versus mefloquine (PACOME) clinical trial who were followed until delivery. Cytokine concentrations were measured by flow cytometrybased multiplex bead array. Significantly elevated levels of IL-10 and lower levels of TNF-α were observed at delivery compared with inclusion (P = 0.005). At inclusion, the presence of circulating IFN-γ, a higher CD4 + T cell count and having initiated intermittent preventive treatment of malaria with sulfadoxine pyrimethamine (SP-IPTp) were all associated with a lower likelihood of Plasmodium falciparum infection. At delivery, the inverse relationship between the presence of infection and circulating IFN-γ persisted, although there was a positive association between the likelihood of infection and the presence of circulating TNF-α. Initiation of antiretroviral therapy was associated with elevated IL-5 production. Consistent with our own and others' observations in HIV seronegative subjects, this study shows circulating IL-10 to be a marker of infection with P. falciparum during pregnancy even in HIV-infected women, although plasma IFN-γ may be a marker of anti-malarial protection in such women.

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Section: Discussioncontrasting

confidence: 43%

Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Ibitokou¹,

Denoeud-Ndam²,

Ezinmègnon³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…IL-10, an immunosuppressive cytokine able to suppress Tcell and macrophage cytokine production (36), is expressed by T cells, B cells, and macrophages (24). In HIV infection, elevated IL-10 levels (1, 36) play an important role in the pathogenesis of HIV and AIDS by causing more efficient infection of macrophages (32) and increased viral replication in T cells and macrophages (37) and by impairing antigen presentation by macrophages (25).…”

Section: Vol 7 2000mentioning

confidence: 99%

Down Regulation of CD4 Expression following Isolation and Culture of Human Monocytes

Graziani-Bowering

Filion

2000

Clin Diagn Lab Immunol

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The down regulation of CD4 by cultured monocytes has been observed by our group and by other investigators. Flow cytometric experiments were done to examine which factors might influence this phenomenon. The addition of lipopolysaccharide, granulocyte-macrophage colony-stimulating factor, macrophage colonystimulating factor, or interleukin-10 to monocyte cultures failed to inhibit the decrease in monocyte CD4 expression routinely observed following overnight culture. The down regulation was an adherence-independent phenomenon and was not influenced by the type of anticoagulant into which the peripheral blood was collected or by the presence or absence of lymphocytes within the cultures. The avoidance of the use of FicollPaque to isolate peripheral blood mononuclear cells did not prevent monocyte CD4 down regulation. Finally, by tagging monocyte CD4 with an anti-CD4 phycoerythrin-conjugated monoclonal antibody prior to culture, we were able to determine that the down regulation observed was the result of the internalization of the molecule. At this time, we conclude that the observed down regulation of monocyte CD4 is probably due to the differentiation of blood monocytes into tissue culture-derived macrophages rather than to some artifact of the isolation procedure.

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“…In addition, dichotomous effects on replication have been observed for IL-4, IL-10, interferon-gamma (IFN-g) and transforming growth factorbeta (TGF-b) (reviewed in [52]). For example, the inhibition of virus replication induced by IL-10 in human macrophages [53,54] has been linked to inhibition of cell differentiation [55], inhibition of reverse transcription [56] and inhibition of TNF-a and IL-6 secretion [57]. In contrast, IL-10 can synergize with TNF-a to activate HIV replication in monocytic cells [58].…”

Section: Human Macrophagesmentioning

confidence: 99%

“…Finally, IL-4 can suppress virus replication in human macrophages and can synergize with IL-10 in this suppression [54]. In contrast, IL-4 and IFN-g have been reported to increase HIV-1 replication in macrophages [56].…”

Section: Human Macrophagesmentioning

confidence: 99%

CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-α) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive

Copeland

McKAY

Newton

et al. 1999

Clinical and Experimental Immunology

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SUMMARYHIV replication and LTR-mediated gene expression can be modulated by CD8 þ T cells in a cell typedependent manner. We have previously shown that supernatants of activated CD8 þ T cells of HIVinfected individuals greatly enhanced p24 levels in human macrophages infected with NSI or SI primary isolates of HIV-1. Here we have examined the effect of culture with CD8 þ T cell supernatants on HIV-1 LTR-mediated gene expression in monocytic cells. CD8 þ T cell supernatants enhanced LTR-mediated gene expression in U38 cells activated with Tat in the absence or presence of phorbol myristate acetate (PMA) and ionomycin or TNF-a. Further, enhancement of LTR-mediated gene expression and virus replication in U38 cells and U1 cells, respectively, was pertussis toxin-sensitive. The enhancement of gene expression and virus replication was associated with increased levels of TNF-a and was significantly abrogated by antibody to TNF-a. In contrast, the suppression of LTR-mediated gene expression by CD8 þ T cell supernatants in Jurkat T cells was not pertussis toxin-sensitive and TNF-a levels were not affected. These results demonstrate that factors produced by CD8 þ T cells utilize different cellular pathways to mediate their effects on HIV transcription and replication in different cell types.

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Interleukin-10 inhibits initial reverse transcription of human immunodeficiency virus type 1 and mediates a virostatic latent state in primary blood-derived human macrophages in vitro

Cited by 53 publications

References 32 publications

Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Insights Into Circulating Cytokine Dynamics During Pregnancy in HIV-Infected Beninese Exposed to Plasmodium falciparum Malaria

Down Regulation of CD4 Expression following Isolation and Culture of Human Monocytes

CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-α) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive

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