Interleukin‐10 inhibits B7 and intercellular adhesion molecule‐1 expression on human monocytes

Abstract

There is evidence that interleukin -10(IL-10) interferes with the costimulatory properties of antigen-presenting cells and, thereby, inhibits their ability to induce T cell activation. To determine whether this effect might involve modulation of the expression of accessory molecules, we analyzed by flow cytometry the influence of human IL-10 on the basal expression of intercellular adhesion molecule 1 (ICAM-1) as well as on the interferon gamma (IFN-gamma)-induced up-regulation of ICAM-1 and B7 at the surface of human monocytes. IL-10 inhibited both the basal expression and the IFN-gamma-induced ICAM-1 up-regulation. IL-10 also reduced B7 up-regulation on IFN-gamma-stimulated monocytes. The inhibitory effect of IL-10 both on ICAM-1 and B7 expression was shown to be dose dependent. We conclude that the ability of IL-10 to decrease both ICAM-1 and B7 expression on monocytes might contribute to its immunosuppressive properties.

Allium sativum (garlic) suppresses leukocyte inflammatory cytokine production in vitro: Potential therapeutic use in the treatment of inflammatory bowel disease

“…Specific antagonists such as IL-10 control these proinflammatory cytokines. IL-10 is a potent suppressor of antigen presentation and T-cell activation (19). IL-10 inhibits the production of proinflammatory cytokines such as TNF-␣ and IL-6 in cells of the monocyte-macrophage lineage (20,21) and their ability to express MHC class II molecules (20) or IL-12 (22).…”

Immune Response of Cattle Infected with African Trypanosomes

“…IL-10 also acts to downregulate expression of the B7 costimulatory molecules on murine antigen-presenting cells (Willems et al 1994), which may lead to T cell anergy and in some cases apoptosis (Dubois et al 1994). Anergic T cells do not proliferate or produce IL-2, but retain the ability to produce other cytokines such as IFN-g (Jenkins et al 1990).…”

Lipid-mediated gene transfer of viral IL-10 prolongs vascularized cardiac allograft survival by inhibiting donor-specific cellular and humoral immune responses

“…10,11 In addition to inhibiting cytokine production, IL-10 has also been shown to block accessory cell function of macrophages by down-regulating MHC class II expression and decreasing expression of the costimulatory molecule B7. [12][13][14][15] As a result of these activities, IL-10 is a potent suppressor of effector functions of macrophages and T cells. However, in addition to regulating immune responses negatively, IL-10 also has growth factor activity and promotes the proliferation and differentiation of B cells, mast cells and thymocytes.…”