Interleukin‐10 expression significantly correlates with minor CD8⁺ T‐cell infiltration and high microvessel density in patients with gastric cancer

Abstract

We aimed to investigate the relationships between interleukin-10 (IL-10) expression and both the clinicopathological findings and prognoses in patients with gastric cancer and to compare IL-10 expression with microvessel (MV) density and CD81 T lymphocyte infiltration to evaluate its effects on angiogenesis and immune responses in gastric cancer. IL-10 expression was determined in gastric cancer patients by reverse transcription-polymerase chain reaction (RT-PCR) or immunohistochemical procedures. Two of 7 normal gastric tissues showed IL-10 mRNA expression, while its expressions were confirmed in all advanced gastric carcinoma tissues examined (n 5 11) by RT-PCR. Immunohistochemical staining demonstrated that IL-10 expression was detected in 52 (47.7%) of 109 cases. There was a close correlation between IL-10 expression and MV density. IL-10 expression inversely correlated with CD81 T-lymphocyte infiltration. The prognoses of patients whose tumors expressed IL-10 were significantly worse than those of patients whose tumors did not express IL-10. Multivariate analysis indicated IL-10 expression was an independent prognostic factor. IL-10 might be associated with tumor progression by stimulating angiogenesis and suppressing immune responses in gastric cancer. ' 2005 Wiley-Liss, Inc.

Serum interleukin-6 and -10 levels in patients with gastric cancer

“…Matsuguchi et al [27] reported that high serum IL-10 levels in patients with hepatocellular carcinoma presumably also resulted from the secretion of IL-10 by tumor cells. Sakamoto et al [28] demonstrated that IL-10 expression was found in 48% of GC tissues by immunohistochemistry. They found that tumor IL-10 expression was strongly correlated with depth of tumor invasion and with lymph node metastasis, and that IL-10 expression was inversely correlated with CD8+ T-lymphocyte infi ltration.…”

CD4+ regulatory T cells in gastric cancer mucosa are proliferating and express high levels of IL-10 but little TGF-β

“…Previously, we have shown that both blood and gastric T cells from gastric cancer patients produce high levels of IL-10 after in vitro stimulation [46]. Furthermore, in a recent study it was shown that IL-10 production by gastric cancer cells correlates with dramatically reduced survival [47].…”

Characterization and Regulation of Bv8 in Human Blood Cells

“…Therefore, Bv8 produced by these cell types may contribute to angiogenesis and might also have additional regulatory roles that remain to be defined. Originally described as a cytokine produced by T helper 2 cells (52), IL-10 has been also implicated in angiogenesis (53,54).…”