Interleukin-1 α Alters the Expression of Matrix Metalloproteinases and Collagen Degradation by Pulp Fibroblasts

Expressional Alterations of Fibrillin-1 during Wound Healing of Human Dental Pulp

“…The temporal and spatial expression of MMPs and their natural inhibitors, the tissue inhibitor of metalloproteinases, likely contribute to tooth morphogenesis and pulp tissue development (13). Moreover, MMP-1, MMP-2, and MMP-3 play a role in tissue destruction in inflamed human dental pulp (14,15), and MMP-2 (16) and MMP-3 (17) promote wound healing in injured rat pulp. Several MMPs are able to digest fibrillins; MMP-2, -3, -9, -12, -13, and -14 are known to degrade fibrillin-1, and MMP-2, -12, and -13 degrade fibrillin-2 (18,19).…”

Inflammation in the pleural cavity following injection of multi-walled carbon nanotubes is dependent on their characteristics and the presence of IL-1 genes

“…In the IL1-KO mice, Timp1 expression was low in the mice treated with CNT-2 and asbestos. TIMP1 is found to be positively regulated by IL-1 in other studies (Wilczynska et al, 2006;Wisithphrom, Murray, and Windsor 2006;. However, a strong increase in its expression was observed in the pleura and lungs in IL1-KO mice exposed to CNT-1.…”

Dentin Matrix Protein-1 Activates Dental Pulp Fibroblasts

“…Human impacted teeth from 8 subjects were also collected for pulp cell isolation. Cells were obtained from explanted pulp tissue as previously described (22). In brief, the teeth were cleaned with ethanolcontaining gauze, the apical 2 mm was cut, and teeth were decoronated under sterile conditions.…”

“…Staining for immunofluorescence studies was performed as described previously (22) by using goat polyclonal anti-vimentin antibody 1:25 (Chemicon International, Temecula, CA) and a rabbit polyclonal anti-collagen 1a1 1:25 (SantaCruz Biotechnology Inc, Santa Cruz, CA), followed by Texas red conjugated anti-goat and Alexa-fluor 647 conjugated anti-rabbit secondary antibodies, respectively. Slides were mounted with the nuclear staining dye DAPI (Slow fade Gold Antifade reagent with DAPI; Invitrogen, Carlsbad, CA) and kept in the dark for evaluation by confocal microscopy.…”

“…To test this hypothesis, we activated dental pulp fibroblasts with different concentrations of DMP-1 and measured IL-6 and IL-8 release. Primary pulp fibroblasts were isolated and cultured as described previously (22). These cells were positive by immunofluorescence for vimentin ( Fig.…”