Interleukin-1, Tumor Necrosis Factor and Lipopolysaccharide Additively Stimulate Production of Adrenomedullin in Vascular Smooth Muscle Cells

Sugo, S.; Minamino, Naoto; Shoji, Hiromichi; Kangawa, Kenji; Kitamura, Koji; Eto, Tomoo; Matsuo, Hirofumi

doi:10.1006/bbrc.1995.1148

Cited by 316 publications

(166 citation statements)

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“…We used three anti-AM monoclonal antibodies (23,24): one recognized the ring structure (rAdR-2; anti-rat AM [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]), one the C-terminal amide structure (AdC-1; anti-AM[46-52]NH2), and one the middle portion (AdM-2; anti-AM [25][26][27][28][29][30][31][32][33][34][35][36]) of the molecule. These antibodies were gifted from Shionogi & Co., Ltd., Osaka, Japan.…”

Section: Preparation Of Antibodiesmentioning

confidence: 99%

“…Monoclonal mouse anti-AM [12][13][14][15][16][17][18][19][20][21][22][23][24][25]Fab was conjugated with 6-maleimidohexanoyl-2,4 DNP-biotinyl-BSA and used as a capture antibody (26,27 (26,27).…”

Section: Preparation Of Capture Antibody and Antibody-labeled Enzymementioning

confidence: 99%

“…To measure rat AM-m, an aliquot (10 µl) of standard AM-m, plasma sample or tissue extract was mixed with 140 µl of AM buffer containing 0.1% Triton X-100, 50 KIU/ml of aprotinin, 10 fmol of 2,4-DNP-biotinyl-BSA-anti-AM [12][13][14][15][16][17][18][19][20][21][22][23][24][25] Fab conjugate (as a capture antibody) and 3 fmol of anti-AM[46-52]NH2 Fab -β-D-galactosidase conjugate (as an antibody-enzyme) and incubated overnight at 4 ºC without shaking (first incubation; Formation). Thereafter, the mixture was incubated with one streptavidin-coated polystyrene bead for 30 min at room temperature with shaking (210 strokes/min, second incubation; Entrapment).…”

Section: Two-site Immunoenzymometric Assay (Iema) For Rat Am-m and Am-tmentioning

confidence: 99%

“…1 (25, 27). AM was reacted simultaneously with 2,4-DNP-biotinyl-BSA-anti-AM [12][13][14][15][16][17][18][19][20][21][22][23][24][25] Figure 2 shows a typical calibration curve for rat AM-Gly, AM-m and AM-t. The lower detection limit of AM-Gly was 1 amol/assay; the assay range for plasma was 50 fmol/l (20-µl plasma samples).…”

Section: Immune Complex Transfer Enzyme Immunoassay For Rat Am-glymentioning

confidence: 99%

“…In this regard, Nishikimi et al reported that AM-m is produced in various organs and released into the blood, and that it is likely cleared by the lungs (20). In addition, substances such as tumor necrosis factor, interleukin-1, and lipopolysaccharide (LPS) have been shown to stimulate AM gene transcription and protein synthesis (21,22).…”

Section: Introductionmentioning

confidence: 99%

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Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

et al. 2003

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Section: Preparation Of Antibodiesmentioning

confidence: 99%

Section: Preparation Of Capture Antibody and Antibody-labeled Enzymementioning

confidence: 99%

Section: Two-site Immunoenzymometric Assay (Iema) For Rat Am-m and Am-tmentioning

confidence: 99%

Section: Immune Complex Transfer Enzyme Immunoassay For Rat Am-glymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

et al. 2003

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The Pivotal Role of Adrenomedullin in Producing Hyperdynamic Circulation During the Early Stage of Sepsis

Wang

Ba²,

Cioffi³

et al. 1998

Arch Surg

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Background: Initial cardiovascular responses during sepsis are characterized by hyperdynamic circulation. Although studies have shown that a novel potent vasodilatory peptide, adrenomedullin (ADM), is up-regulated under such conditions, it remains unknown whether ADM is responsible for initiating the hyperdynamic response. Objective: To determine whether increased ADM release during early sepsis plays any major role in producing hyperdynamic circulation. Design, Intervention, and Main Outcome Measure: Synthetic rat ADM (8.5 µg/kg of body weight) was infused intravenously in normal rats for 15 minutes at a constant rate.Cardiacoutput,strokevolume,andmicrovascularblood flow in various organs were determined immediately as well as 30 minutes after ADM infusion. At 30 minutes after infusion, plasma ADM level was also measured. In additional groups, rats were subjected to sepsis by cecal ligation and puncture. At 1.5 hours after cecal ligation and puncture, specific anti-rat ADM antibodies were infused, which completely neutralized the circulating ADM. Various hemodynamic variables were measured 5 hours after cecal ligation and puncture (ie, the early stage of sepsis). Results: Cardiac output, stroke volume, and microvascular blood flow in the liver, small intestine, kidney, and spleen increased, and total peripheral resistance decreased 0 and 30 minutes after ADM infusion. In addition, plasma levels of ADM increased from the preinfusion level of 92.7 ± 5.3 to 691.1 ± 28.2 pg/mL 30 minutes after ADM infusion, which was similar to ADM levels observed during early sepsis. Moreover, 5 hours after the onset of sepsis, cardiac output, stroke volume, and microvascular blood flow in various organs increased and total peripheral resistance decreased. Administration of anti-ADM antibodies, however, prevented the occurrence of the hyperdynamic response. Conclusions: The results suggest that increased ADM production and/or release plays a major role in producing hyperdynamic responses during early sepsis. Since our previous studies have shown that vascular responsiveness to ADM decreases in late sepsis, maintenance of ADM vascular responsiveness by pharmacological agents during the course of sepsis may prevent transition from the hyperdynamic to the hypodynamic state.

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Plasma adrenomedullin in rheumatoid arthritis compared with other rheumatic diseases

Yudoh¹,

Matsuno²,

Kimura³

1999

Arthritis & Rheumatism

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Interleukin-1, Tumor Necrosis Factor and Lipopolysaccharide Additively Stimulate Production of Adrenomedullin in Vascular Smooth Muscle Cells

Cited by 316 publications

References 0 publications

Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

The Pivotal Role of Adrenomedullin in Producing Hyperdynamic Circulation During the Early Stage of Sepsis

Plasma adrenomedullin in rheumatoid arthritis compared with other rheumatic diseases

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