Interleukin-1 single nucleotide polymorphisms as risk factors for susceptibility of inflammatory bowel disease: an Iraqi Arab population-based study

Ad’hiah, Ali H.; Hessan, Ebtssam B.; Shahab, Betool A.

doi:10.1080/20905068.2019.1592938

Cited by 4 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, IL-1β promotes Th17 responses, suggesting synergistic interactions with IL-23 signals that sustain innate and adaptive inflammatory responses in the gut; moreover, the IL-1β regulation of Th17 can be induced by a MyD88-dependent microbial signal [74]. In addition, in this case, particular IL1B polymorphisms were significantly associated with the risk of IBD [75,76], conferring to the IL-1β drug silencing a potential therapy role, especially for patients caring these variants or for those with deep ulceration and who do not respond to several different current therapeutics [77]. In this study, an increase in IL1B gene expression in Caco-2 cells and IL-1β release for both Caco-2 and THP-1 cells was observed after pro-inflammatory stimuli induction.…”

Section: Discussionmentioning

confidence: 94%

Cow Milk Extracellular Vesicle Effects on an In Vitro Model of Intestinal Inflammation

et al. 2022

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are lipid bilayer nano-dimensional spherical structures and act mainly as signaling mediators between cells, in particular modulating immunity and inflammation. Milk-derived EVs (mEVs) can have immunomodulatory and anti-inflammatory effects, and milk is one of the most promising food sources of EVs. In this context, this study aimed to evaluate bovine mEVs anti-inflammatory and immunomodulating effects on an in vitro co-culture (Caco-2 and THP-1) model of intestinal inflammation through gene expression evaluation with RT-qPCR and cytokine release through ELISA. After establishing a pro-inflammatory environment due to IFN-γ and LPS stimuli, CXCL8, IL1B, TNFA, IL12A, IL23A, TGFB1, NOS2, and MMP9 were significantly up-regulated in inflamed Caco-2 compared to the basal co-culture. Moreover, IL-17, IL-1β, IL-6, TNF-α release was increased in supernatants of THP-1. The mEV administration partially restored initial conditions with an effective anti-inflammatory activity. Indeed, a decrease in gene expression and protein production of most of the tested cytokines was detected, together with a significant gene expression decrease in MMP9 and the up-regulation of MUC2 and TJP1. These results showed a fundamental capability of mEVs to modulate inflammation and their potential beneficial effect on the intestinal mucosa.

show abstract

Section: Discussionmentioning

confidence: 94%

Cow Milk Extracellular Vesicle Effects on an In Vitro Model of Intestinal Inflammation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In UC, the stimulation of white blood cells arises after bacterial imbalance and infection in the gut, along with an impaired mucosal barrier. Even if the infection subsides, the response of the immune system continues, leading to chronicity (3,4). One of the scoring systems that clinicians use to estimate the clinical severity of UC is the Simple Clinical Colitis Activity Index (SCCAI), which offers an easy way of detecting possible relapse and decreases the need for invasive and uncomfortable examinations or laboratory tests (5,6).…”

Section: Introduction Introductionmentioning

confidence: 99%

Impact of Cyclooxygenase-2 Polymorphism -765G>C (rs20417) on the Development of Ulcerative Colitis

Alahmed,

A. Al-Rubaee

2023

SGO

View full text Add to dashboard Cite

Background: Ulcerative Colitis is chronic, progressive disease affecting large intestine. Cyclooxygenase-2 enzyme is an inducible enzyme that has important role in the process of inflammation. Ulcerative Colitis pathogenesis is comprising chronic inflammation; any alteration in the gene responsible for cuclooxygenase-2 expression such as -765G>C (rs20417) polymorphism may affect risk of having the disease. Objectives: To record and evaluate the prevalence of -765G>C (rs20417) polymorphism in group of Ulcerative Colitis Iraqi patients and correlation it with disease clinical severity. Method: A case-control, single-center study included ninety participants collected from Gastroenterology and Hepatology Hospital/Basrah/Iraq, divided in to 30 newly diagnosed patients, 30 previously diagnosed patients and 30 healthy controls. Genetic analysis done through dual color discrimination assay. Results: To our knowledge the present study is the first report in Iraqi population that investigates the correlation between COX-2 polymorphism, rs20417 with risk of developing UC. The prevalence of the -765G and -765C alleles was 85.8% and 14.1% in the patient group and 86.6% and 13.3% in the control group respectively, the -765CC variant in Basrah city was infrequent in population of UC patients (14.1%) and in controls (13.3%). There was no statistical difference in the disease association and in the relationship to the disease severity but GG genotypes showed more sever clinical disease than those with other variants. Conclusion:In disparity to other reports on the role of COX-2 polymorphisms at position -765 in the term of modifying the risk for UC in a group of Arab Iraqi patients, we did not find significant results for this variation's association but in term of association with the level of disease clinical severity, carriers of GG wild type had more active clinical disease than those with mutant variants.

show abstract

Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq

et al. 2021

View full text Add to dashboard Cite

The interleukin-1 family has multifaceted roles in men ٫ s reproductive syste. Out of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased. The current study aims to verify a possible linkage of Variable Number Tandem Repeat (VNTR) polymorphism of the IL-1RN gene with human men infertility. The study groups enrolled included 100 infertile men and 100 fertile and healthy men. Their seminal fluids were subjected to analysis. Also peripheral blood samples were collected for the assessment or detection of polymorphic Variable Number Tandem Repeats (VNTR) polymorphism of interleukin-1 receptor antagonist gene (IL-1RN). Two alleles, namely IL-1RN1 allele corresponding to 410bp fragment and IL-11RN2 that corresponding to 240bp fragments, are a marker for human men infertility, detected by PCR technique. The results delineated a high frequency of IL-1RN2 allelic gene variants (26%), and two VNTR allelic gene variants carriers IL-1RN1 and IL-1RN2 (16%) among infertile men with significant impacts on sperm motility and morphology (P< 0.000-0.002) respectively. This prospective study in Kurdistan region (Erbil-Iraq) defined a significant impact of VNTR polymorphism of IL-1RN gene in the etiology of men infertility especially on sperm motility and morphology; particularly carriers of IL-1RN2 allelic variants.

show abstract

Interleukin-1 single nucleotide polymorphisms as risk factors for susceptibility of inflammatory bowel disease: an Iraqi Arab population-based study

Cited by 4 publications

References 25 publications

Cow Milk Extracellular Vesicle Effects on an In Vitro Model of Intestinal Inflammation

Cow Milk Extracellular Vesicle Effects on an In Vitro Model of Intestinal Inflammation

Impact of Cyclooxygenase-2 Polymorphism -765G>C (rs20417) on the Development of Ulcerative Colitis

Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq

Contact Info

Product

Resources

About