Interleukin-1 Receptor Antagonist and Interleukin-1 Beta Levels in Equine Synovial Fluid of Normal and Osteoarthritic Joints: Influence of Anatomic Joint Location and Repeated Arthrocentesis

Lasarzik, Juliane; Lischer, Christoph; Ehrle, Anna; Estrada, Roberto; Rettig, Matthias; Klaus, C.; Einspanier, Ralf; Bondzio, Angelika

doi:10.1016/j.jevs.2016.03.017

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…While there was a positive correlation between IL-1β and IL-1Ra for the commercial containers this was not the case for the non-commercial containers. This positive correlation of IL-1β to IL-1Ra for the commercial method mirrors a similar correlation observed in joints with naturally occurring osteoarthritis, which may be due to a counter induction of IL-1Ra, in response to an increase in IL-1β (22). Despite the presence of positive correlation for the commercial containers, both production systems had increases of IL-1Ra in comparison to IL-1β resulting in IL-1β/IL-1Ra ratios below the 1:100 considered to be of therapeutic value (1:2.65 and 1:1.34, respectively).…”

Section: Commercial and Non-commercial Acs Preparations: Cytokine Analysissupporting

confidence: 76%

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Hale

Hughes

et al. 2021

Front. Vet. Sci.

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Autologous conditioned serum (ACS) is a common intra-articular treatment for osteoarthritis in horses. The objective of this study was to investigate the influence of ACS preparation method on product contamination and concentrations of relevant cytokines and the influence of multiple freeze/thaw cycles. Blood was obtained from 10 healthy Thoroughbred horses and processed in parallel using a commercial and a non-commercial method to obtain ACS. Fluorescent microsphere immunoassay (FMIA) analysis was performed to quantify Interleukin 1 receptor antagonist (IL-1Ra), Interleukin-10 (IL-10), Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) concentrations in ACS obtained by both production methods. Effect of 3, 4 and 5 freeze/thaw cycles on concentrations of IL-1Ra, IL-10, IL-1β and TNF-α were assessed against baseline samples (2 cycles) in commercial ACS products. Standard aerobic and anaerobic culture methods were applied to both ACS products. Mixed effect one-way analyses of variance (ANOVA) were used to compare the two ACS production method for each cytokine. Repeated measures, mixed effect ANOVA were used to assess the effect of freeze/thaw on cytokine concentrations. Significance was set at P < 0.05. There was no difference in cytokine concentration between production methods (IL-1Ra P = 0.067, IL-1β P = 0.752, IL-10 P = 0.211 and TNF-α P = 0.25). Microbial growth was only observed in two samples obtained using the commercial production method. When compared to baseline, IL-1Ra concentration was decreased following the 5th freeze/thaw cycle (P < 0.001). These results suggest that the concentration of important cytokines are not influenced by ACS production method. When storing ACS samples for future use, freeze/thaw cycles associated with standard clinical practice are unlikely to influence cytokine concentrations. However, the lack of outcome measures associated with 1 or 2 freeze/thaw cycles represents a limitation of this study.

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Section: Commercial and Non-commercial Acs Preparations: Cytokine Analysissupporting

confidence: 76%

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Hale

Hughes

et al. 2021

Front. Vet. Sci.

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“…High variability was detected in the clinical read-outs. Lasarzik et al [67] argue that white blood cell counts and total protein assay in synovial fluid have a large range of values and may not discriminate healthy and mildly arthritic joints. Indeed, no differences in lameness and effusion scores nor in carpal circumference were noted following administration of NP dispersions, albeit with low numbers of animals, so the model will need to be further assessed to see if it has sufficient discriminatory power to rank order therapeutic and regenerative interventions or if it is limited to qualitative conclusions on safety and efficacy.…”

Section: Discussionmentioning

confidence: 99%

Intra-articular delivery of a nanocomplex comprising salmon calcitonin, hyaluronic acid, and chitosan using an equine model of joint inflammation

Sladek

Kearney

Crean

et al. 2018

Drug Deliv. and Transl. Res.

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Polyelectrolyte nanoparticle constructs (NPs) comprising salmon calcitonin (sCT), chitosan (CS), and hyaluronic acid (HA) were previously established as having anti-inflammatory potential when injected via the intra-articular (i.a.) route to a mouse model. We attempted to translate the formulation to a large animal model, the lipopolysaccharide (LPS)-stimulated equine model of joint inflammation. The aim was to manufacture under aseptic conditions to produce sterile pyrogen-free NPs, to confirm physicochemical characteristics, and to test toxicity and efficacy in a pilot study. NP dispersions were successfully formulated using pharmaceutical-grade source materials and were aseptically manufactured under GMP-simulated conditions in a grade A modular aseptic processing workstation. The NP formulation had no detectable pathogen or endotoxin contamination. NPs were then tested versus a lactated Ringer's solution control following single i.a. injections to the radiocarpal joints of two groups of four horses pre-treated with LPS, followed by arthrocentesis at set intervals over 1 week. There was no evidence of treatment-related toxicity over the period. While there were no differences between clinical read-outs of the NP and the control, two synovial fluid-derived biomarkers associated with cartilage turnover revealed a beneficial effect of NPs. In conclusion, NPs comprising well-known materials were manufactured for an equine i.a.-injectable pilot study and yielded no NP-attributable toxicity. Evidence of NP-associated benefit at the level of secondary endpoints was detected as a result of decreases in synovial fluid inflammatory biomarkers.

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“…Concentrações altas de IL-1ra no líquido sinovial são tidas como uma resposta natural do organismo, como já demonstrado em estudos de artrite reumatoide e OA (LASARZIK et al, 2016;MACHADO et al, 2019).…”

Section: Mediadores Anti-inflamatóriosunclassified

>b<Avaliação dos efeitos >i<in vitro>/i< do dimetilsulfóxido em células sinoviais de equinos e de sua utilização intra-articular como tratamento para sinovite aguda>/b<

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Interleukin-1 Receptor Antagonist and Interleukin-1 Beta Levels in Equine Synovial Fluid of Normal and Osteoarthritic Joints: Influence of Anatomic Joint Location and Repeated Arthrocentesis

Cited by 7 publications

References 27 publications

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum

Intra-articular delivery of a nanocomplex comprising salmon calcitonin, hyaluronic acid, and chitosan using an equine model of joint inflammation

>b<Avaliação dos efeitos >i<in vitro>/i< do dimetilsulfóxido em células sinoviais de equinos e de sua utilização intra-articular como tratamento para sinovite aguda>/b<

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