Interleukin-1: local production and modulation of human granulosa luteal cells steroidogenesis

Barak, Vivian; Yanai, Peter; Treves, Abraham J.; Roisman, Isaac; Simon, Alexander; Laufer, Neri

doi:10.1016/s0015-0282(16)55318-7

Cited by 52 publications

(14 citation statements)

References 25 publications

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“…In contrast, others have observed no significant effect of IL-2 or IL-3 on FSH-induced estradiol production by cultured rat granulosa cells [26]. In comparison, no significant effect of IL-6 was observed on basal or hCG-induced progesterone or estradiol production by porcine luteal cells [30] whereas IL-1 was found to inhibit hCG-induced estradiol production by both porcine [30] and human [29,31] luteal cells. Also, IL-1 inhibited hCG-induced androsterone production by rat whole ovarian dispersates whereas IL-2 and IL-6 had no effect [32].…”

Section: Discussionmentioning

confidence: 84%

Effects of Interleukin-6 on Proliferation and Follicle-Stimulating Hormone-Induced Estradiol Production by Bovine Granulosa Cells in Vitro: Dependence on Size of Follicle1

Alpízar

Spicer

1994

Biology of Reproduction

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The objective of the present studies was to determine the effect of IL-6 on proliferation and FSH-induced estrogen production by granulosa cells from small (1-5 mm) and large (> or = 8 mm) bovine follicles. FSH-induced estradiol production by granulosa cells from large follicles (expressed as pg estradiol/10(5) cells/24 h) was not affected (p > 0.05) by 0.1, 1.0, or 10 ng/ml of interleukin (IL)-6, whereas 30 and 100 ng/ml of IL-6 inhibited (p < 0.05) FSH-induced estradiol production by 32% in cells from large follicles. In comparison, all doses of IL-6 inhibited (p < 0.05) FSH-induced estradiol production in cultures of cells from small follicles; maximal inhibition was achieved (75% decrease) with > or = 10 ng/ml. IL-6 was also a more potent inhibitor of cell proliferation in cultures of cells from small (55% inhibition) than from large (32% inhibition) follicles. In summary, IL-6 has a more potent inhibitory effect on proliferation and FSH-induced estradiol production by bovine granulosa cells collected from small follicles than from large follicles. Thus, it appears that less differentiated granulosa cells (small follicles) are more sensitive to IL-6 than are highly differentiated granulosa cells (large follicles).

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Section: Discussionmentioning

confidence: 84%

Effects of Interleukin-6 on Proliferation and Follicle-Stimulating Hormone-Induced Estradiol Production by Bovine Granulosa Cells in Vitro: Dependence on Size of Follicle1

Alpízar

Spicer

1994

Biology of Reproduction

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“…This observation not only confirmed that the intrafollicular injection did not alter follicle maturation, as previously concluded, but also pointed out the role of IL-1␤ in the ovulation process in the mare. In other species, the involvement of IL-1␤ in ovulation-associated events such as the modulation of steroidogenesis [53], regulation of prostaglandin [54], and protease synthesis [31,33] have been demonstrated. Such implications of IL-1␤ could also exist in the mare.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Effect of Epidermal Growth Factor, Interleukin-1β, and Interleukin-1RA on Equine Preovulatory Follicles1

Martoriati

Duchamp

Gérard

2003

Biology of Reproduction

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Paracrine factors have significant effects during folliculogenesis. Because of various morphological features, the mare is a convenient model to study in vivo the effects of factors involved in periovulatory events. In the present work, epidermal growth factor (EGF; experiment 1, n = 49 mares) and interleukin-1beta and interleukin-1RA (IL-1beta and IL-1RA, respectively; experiment 2, n = 80 mares) were injected intrafollicularly to evaluate the influence of these factors on in vivo maturation of equine preovulatory follicles. A transvaginal ultrasound-guided injection was performed when the diameter of the dominant follicle reached 30-34 mm. In experiment 1, the four experimental groups were 1) EGF group, intrafollicular (i.f.) injection of EGF (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum; 2) control group, no injection; 3) PBS group, i.f. injection of 2 ml of PBS plus i.v. injection of physiological serum; 4) crude equine gonadotropins (CEG) group, i.f. injection of PBS plus i.v. injection of CEG (20 mg). In experiment 2, groups 3 and 4 were the same as in experiment 1, but groups 1 and 2 were changed as follows: 1) IL-1beta group, i.f. injection of IL-1beta (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum; 2) IL-1RA group, i.f. injection of IL-1RA (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum. In each experiment, cumulus-oocyte complexes from dominant/injected follicles were collected by transvaginal ultrasound-guided aspiration 38 h after intrafollicular injection. Cumulus morphology and oocyte nuclear stage were assessed. Additionally, in experiment 2, 40 mares were used to determine the time of ovulation after treatments. Our results indicate that intrafollicular injection of EGF or PBS induced lower cumulus expansion and oocyte maturation rates compared with the CEG group (P < 0.05). In experiment 2, the IL-1beta and CEG groups showed the same expansion rate, the same oocyte maturation rate, and the same ovulation distribution. On the other hand, the intrafollicular injection of IL-1RA, as PBS, did not induce follicle and cumulus-oocyte complex (COC) maturation. In conclusion, we confirmed that the technique of intrafollicular injection can be used in the mare to study the role of specific molecules. We demonstrated for the first time in mares that the injection of EGF did not influence in vivo COC maturation. In contrast, IL-1beta injection into the dominant follicle induced in vivo oocyte maturation and the ovulation process whereas IL-1RA seemed to block these mechanisms.

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“…The most significant growth factors that have inhibitory effects on ovarian function are epidermal growth factor (EGF/TGF-a), tumour necrosis factor (TNF-) and interleukin 1 (IL-1) (Gougeon, 1996). However, their inhibitory effect can only be detected in the presence of FSH (Hsueh ef al, 1981; Barak et al, 1992;Fukuoka et al, 1992). Because the inhibitor present in the fluid from dominant follicles is also efficient in the absence of FSH, these growth factors are unlikely to play a major role in this process.…”

Section: Discussionmentioning

confidence: 99%

Presence of an aromatase inhibitor, possibly heat shock protein 90, in dominant follicles of cattle

Driancourt¹,

Guet²,

Reynaud³

et al. 1999

Reproduction

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In cattle, it has been suggested that follicular fluid has direct modulatory effects on follicular growth and maturation. In the first part of this study, an in vitro test using aromatase activity of follicular wall fragments as an end point was validated for cattle follicles and was used to test whether follicular fluid (from dominant or non-dominant follicles) modulates aromatase activity. Fluid from dominant follicles at a concentration of 24 or 12% (obtained during the luteal and follicular phases, respectively) significantly inhibited aromatase activity. Inhibitory activity was low or absent in fluid from non-dominant follicles. FSH-stimulated aromatase activity was also reduced by fluid from dominant follicles, but not to a greater extent than in basal conditions. Finally, charcoal-treated fluid from dominant follicles retained its inhibitory activity. In contrast, ovarian venous serum draining a dominant follicle had no activity at the three concentrations tested (6, 12 and 24%). In the second part of the study, identification of the compounds involved in this modulatory activity was attempted using SDS-PAGE. Comparison of the fluorographs from de novo synthesized proteins stored in follicular fluid (inhibitory medium) with those secreted in incubation medium (inactive medium) demonstrated that one protein (90 kDa, pI 5.8) was significantly (P < 0.05) more abundant in fluid from dominant follicles (2.0 +/- 0.09%) than in the culture medium (1.3 +/- 0.1% of the total proteins). This protein had characteristics similar to those of heat shock protein 90 (hsp 90). Therefore, in the final part of the study, the presence of hsp 90 in ovarian cells and follicular fluid was investigated using immunohistochemistry and western blot analysis. After immunohistochemistry, a positive signal was detected mainly in the granulosa cells of larger follicles and to a smaller extent in thecal cells and oocytes. Western blot analysis also demonstrated the presence of hsp 90 in follicular wall fragments and fluid. When blotting was achieved on a sample of follicular fluid resolved by two-dimensional PAGE, the spot detected had a similar location to that at 90 kDa and pI 5.8. Addition of purified hsp 90 to bovine follicles in vitro depressed aromatase activity by altering the K(m) value (and possibly the Vmax value) of the enzyme. It is proposed that hsp 90 is a functional regulator of follicular maturation through its action on aromatase.

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Interleukin-1: local production and modulation of human granulosa luteal cells steroidogenesis

Cited by 52 publications

References 25 publications

Effects of Interleukin-6 on Proliferation and Follicle-Stimulating Hormone-Induced Estradiol Production by Bovine Granulosa Cells in Vitro: Dependence on Size of Follicle1

Effects of Interleukin-6 on Proliferation and Follicle-Stimulating Hormone-Induced Estradiol Production by Bovine Granulosa Cells in Vitro: Dependence on Size of Follicle1

In Vivo Effect of Epidermal Growth Factor, Interleukin-1β, and Interleukin-1RA on Equine Preovulatory Follicles1

Presence of an aromatase inhibitor, possibly heat shock protein 90, in dominant follicles of cattle

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