Interleukin-1 beta stimulates colony-stimulating factor-1 production in human term placenta.

Abstract

The human placenta produces hematopoietic growth factors including colony stimulating factor-1 (CSF-1). We have previously demonstrated Interleukin-1 beta (IL-1 beta) production by decidualized endometrium during pregnancy. Since IL-1 beta stimulates CSF-1 production in a variety of mesenchymal cell types including second trimester villous core mesenchymal cells, the present study was designed to determine if IL-1 beta could also regulate CSF-1 production in term placental explants in vitro. Placental villous explants from normal term placentas (n = 5) were cultured with or without recombinant human IL-1 beta. A dose response relationship between increased added IL-1 beta and increased CSF-1 production as measured by enzyme-linked immunosorbent assay was observed with 1 ng/mL IL-1 beta being the lowest dose to significantly increase CSF-1 production (P < 0.01). In time course experiments, 10 ng/mL maximally induced CSF-1 messenger RNA expression 2.7 fold (P < 0.005) compared to controls at 8 h of culture as determined by dot blot analysis. Production rates of CSF-1 were linear up to 24 h at which time IL-1 beta (10 ng/mL)-treated samples had 1.7-fold higher levels of CSF-1 in the media than nontreated controls (8.38 ng/gm tissue vs. 4.94 ng/gm tissue, P < 0.01). These results demonstrate that IL-1 beta can regulate placental CSF-1 production in vitro and suggest that maternal decidual IL-1 beta may regulate placental CSF-1 production in vivo.

Role for the stem cell factor/KIT complex in schwann cell neoplasia and mast cell proliferation associated with neurofibromatosis

“…The plates were washed with PBST and then 1 pg/ml of sheep anti-rhSCF (Genzyme) was added and incubated for 1 hr at room temperature. Color development was performed using an avidin-biotin-peroxidase Vectastain kit (Vector Laboratories, Inc., Burlingame, CA) and 0-phenylenediamine dihydrochloride as the color substrate as previously described (Kauma, 1993). The plates were read at 490 nm in a V-max Kinetic Microplate Reader (Molecular Devices Corp., Palo Alto, CA).…”

Inhibin and Activin Production in Human Placenta

“…In humans, the placenta produces numerous hormones and growth factors, including human CG (hCG), human placental lactogen (hPL), progesterone (P4), estrogens (estradiol, estrone, estriol, and estetrol) (32,33), specific pregnancy j31 glycoprotein (SP1) (34), renin (35), human chorionic corticotropin (36), human chorionic TSH (37), human chorionic FSH, human uterotropic placental hormone (38), placental LH-releasing factor (39), TSHreleasing factor (40), somatostatin-like substance (41), human nerve growth factor (42), epidermal growth factor (EGF) (43), TGFs (43,44), and insulin-like growth factors (IGFs) (45). Recently, it was found that the placenta also produces cytokines such as interleukin-1 and interleukin-6, which stimulate hCG secretion in trophoblast cells (46)(47)(48)(49)(50). Specific receptors for most of these hormones and factors are present on the membranes of placental cells.…”