Interleukin-1 beta is an irradiation-induced stromal growth factor

Bigildeev, Alexey; Zhironkina, Oxana A.; Lubkova, O. N.; Ni, Drize

doi:10.1016/j.cyto.2013.07.003

Cited by 20 publications

(13 citation statements)

References 19 publications

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“…These findings are reminiscent of the immune response to radiation-induced injury to the lacrimal and salivary glands [41–43]. Several studies showed increased expression of IL-1β following irradiation [44, 45] and established this cytokine as an irradiation-induced stromal growth factor [46]. Our data showed that this cytokine is up-regulated 10- and 6-fold at day 1 and 2 post injury.…”

Section: Resultssupporting

confidence: 71%

RNA-Seq and CyTOF immuno-profiling of regenerating lacrimal glands identifies a novel subset of cells expressing muscle-related proteins

et al. 2017

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The purpose of the present studies was to use CyTOF and RNA-Seq technologies to identify cells and genes involved in lacrimal gland repair that could be targeted to treat diseases of lacrimal gland dysfunction. Lacrimal glands of female BALB/c mice were experimentally injured by intra-glandular injection of interleukin 1 alpha (IL-1α). The lacrimal glands were harvested at various time points following injury (1 to 14 days) and used to either prepare single cell suspensions for CyTOF immuno-phenotyping analyses or to extract RNA for gene expression studies using RNA-Seq. CyTOF immuno-phenotyping identified monocytes and neutrophils as the major infiltrating populations 1 and 2 days post injury. Clustering of significantly differentially expressed genes identified 13 distinct molecular signatures: 3 associated with immune/inflammatory processes included genes up-regulated at days 1–2 and 3 associated with reparative processes with genes up-regulated primarily between days 4 and 5. Finally, clustering identified 65 genes which were specifically up-regulated 2 days post injury which was enriched for muscle specific genes. The expression of select muscle-related proteins was confirmed by immunohistochemistry which identified a subset of cells expressing these proteins. Double staining experiments showed that these cells are distinct from the myoepithelial cells. We conclude that experimentally induced injury to the lacrimal gland leads to massive infiltration by neutrophils and monocytes which resolved after 3 days. RNAseq and immunohistochemistry identified a group of cells, other than myoepithelial cells, that express muscle-related proteins that could play an important role in lacrimal gland repair.

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Section: Resultssupporting

confidence: 71%

RNA-Seq and CyTOF immuno-profiling of regenerating lacrimal glands identifies a novel subset of cells expressing muscle-related proteins

et al. 2017

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“…Adoptive transfer using whole body irradiation with or without head shielding followed by BMT is a cornerstone technique for the study of blood‐borne macrophages vs. microglia . Nevertheless this technique has significant drawbacks such as decreased chimerism when the head is shielded and the use of lethal doses of irradiation, which provokes a long‐term, radiation‐induced systemic increase in proinflammatory cytokines such as Tumour Necrosis Factor‐α, Platelet Derived Growth Factor, chemokines CCL2 and CCR8 , and Interleukin‐1 . Parabiosis proposed by Ajami et al .…”

Section: Discussionmentioning

confidence: 99%

A nonmyeloablative chimeric mouse model accurately defines microglia and macrophage contribution in glioma

Youshani

Wilkinson

et al. 2018

Neuropathology Appl Neurobio

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“…This leads to the dimerization of intracellular TIR complexes, which then engages the MYD88-IRAK4 complex, thereby activating multiple downstream pathways, including NF-κB and p38 MAPK, in multiple organ systems (see Figure 1 ) ( 82 ). IL-1β is an important stromal growth factor in the maintenance of multipotent mesenchymal stromal cells and enhances the ability of stromal cells to maintain HSCs, as shown in both in vitro mouse studies and long term culture-initiating cell assays on human cells ( 83 , 84 ). A study by Pietras et al elucidated the function of IL-1β in normal HSC in vivo .…”

Section: Il-1βmentioning

confidence: 97%

Inflammatory Signaling Pathways in Preleukemic and Leukemic Stem Cells

2017

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Hematopoietic stem cells (HSCs) are a rare subset of bone marrow cells that usually exist in a quiescent state, only entering the cell cycle to replenish the blood compartment, thereby limiting the potential for errors in replication. Inflammatory signals that are released in response to environmental stressors, such as infection, trigger active cycling of HSCs. These inflammatory signals can also directly induce HSCs to release cytokines into the bone marrow environment, promoting myeloid differentiation. After stress myelopoiesis is triggered, HSCs require intracellular signaling programs to deactivate this response and return to steady state. Prolonged or excessive exposure to inflammatory cytokines, such as in prolonged infection or in chronic rheumatologic conditions, can lead to continued HSC cycling and eventual HSC loss. This promotes bone marrow failure, and can precipitate preleukemic states or leukemia through the acquisition of genetic and epigenetic changes in HSCs. This can occur through the initiation of clonal hematopoiesis, followed by the emergence preleukemic stem cells (pre-LSCs). In this review, we describe the roles of multiple inflammatory signaling pathways in the generation of pre-LSCs and in progression to myelodysplastic syndrome (MDS), myeloproliferative neoplasms, and acute myeloid leukemia (AML). In AML, activation of some inflammatory signaling pathways can promote the cycling and differentiation of LSCs, and this can be exploited therapeutically. We also discuss the therapeutic potential of modulating inflammatory signaling for the treatment of myeloid malignancies.

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Interleukin-1 beta is an irradiation-induced stromal growth factor

Cited by 20 publications

References 19 publications

RNA-Seq and CyTOF immuno-profiling of regenerating lacrimal glands identifies a novel subset of cells expressing muscle-related proteins

RNA-Seq and CyTOF immuno-profiling of regenerating lacrimal glands identifies a novel subset of cells expressing muscle-related proteins

A nonmyeloablative chimeric mouse model accurately defines microglia and macrophage contribution in glioma

Inflammatory Signaling Pathways in Preleukemic and Leukemic Stem Cells

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