Interleukin 1 beta and prostaglandin E are involved in the response of periodontal cells to mechanical stress in vivo and in vitro

Saito, Mayumi; Saito, Shigeru; Ngan, Peter; Shanfeld, Joseph; Davidovitch, Zéev

doi:10.1016/0889-5406(91)70005-h

Cited by 185 publications

(118 citation statements)

References 44 publications

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“…These processes are mediated by the P2X7R in other physiological systems. Moreover, external root resorption (ERR), an undesirable side effect of tooth movement, has been associated with the presence of macrophages in several studies (Rygh, 1974;Rygh, 1976;Saito et al, 1991a). In addition, a strong association has been previously reported between IL-1 β and ERR in animal and clinical studies Al-Qawasmi et al, 2003a).…”

Section: Orthodontic Mechanotransduction and The Role Of The P2x7 Recmentioning

confidence: 98%

“…Levels of cytokines such as IL1-β and TNF-α have been associated with enhancement of tooth movement Davidovitch et al, 1988;Hou, Liang & Luo, 1997;Iwasaki et al, 2001;Jager et al, 2005;Lossdorfer, Gotz & Jager, 2002;Saito et al, 1991a;Saito et al, 1991b;Yamaguchi & Kasai, 2005;Yang et al, 2000). These cytokines induce recruitment of endothelial cells (monocytes) that differentiate into osteoclasts after binding of RANKL (receptor activator of nuclear factor kappa ligand), an osteoclast differentiation factor (Ogasawara et al, 2004).…”

Section: Biological Factors In Rate Of Tooth Movement and Errmentioning

confidence: 99%

“…Orthodontic tooth movement involves two fundamental processes possibly mediated by P2X7R: the metabolism of an associated necrotic-type tissue (hyalinized periodontal ligament tissue) and bone modeling/remodeling (Reitan, 1994;Roberts, 2005;Roberts et al, 1981;Rygh, 1974;Rygh, 1976). External root resorption, an undesirable side effect of tooth movement, has been associated with the presence of macrophages, possibly linking it to a chronic inflammatory response (Brudvik & Rygh, 1993a;Brudvik & Rygh, 1993b;Brudvik & Rygh, 1994a;Brudvik & Rygh, 1994b;Rygh, 1974;Rygh, 1976;Saito et al, 1991a). A strong association has been reported between IL-1 beta and root resorption Al-Qawasmi et al, 2003a).…”

Section: Development Of Biomechanical Orthodontic Models Introductionmentioning

confidence: 99%

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Orthodontic mechanotransduction and the role of the P2X7 receptor

Viecilli

Katona

Chen

et al. 2009

American Journal of Orthodontics and Dentofacial Orthopedics

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DEDICATIONTo my parents, who have supported my education. iv ACKNOWLEDGMENTSOne of the most important things I have learned during my PhD is the necessity of the existence of a working network of people that can help you achieve your goals. The more effectively this network functions, the higher the chance you will achieve the goals quickly.I would first like to thank my parents, who have unconditionally supported my decision to abandon a profitable private practice in Orthodontics, and return to humble student conditions while aiming for a full time academic career.I also thank my girlfriend Laura Kruter who has (almost always) been ok with my, some might say, not very sane ideas. Sometimes they do turn out fine, though (like this "PhD" one).I can hardly think of anyone else that could have complemented my work and ideas during this Program as well as Dr. Thomas Katona. While I was passionately conditioned to an idea, he was always rational and careful. While I was sometimes furious with a shortcoming, he was always temperate and humorous. When I ran into his office with some news about a project, he would always listen and almost never seem too busy to see me. While I was working and something went wrong, he would listen and wonder with me about the "whys" and "hows", instead of telling me what to do or express disappointment. He was even subject to some of my home culinary experiments. I was extremely fortunate to find someone that thought about science the same way I did, and that actually helped me with my project instead of just telling me what to do. I felt like we worked as a team, and that helped to increase my motivation during my studies. The third part of this project tested the role of the P2X7 receptor in the dentoalveolar morphology of C57B/6 mice. P2X7R KO (knockout) mice were compared to C57B/6 WT to identify differences in a maxillary molar and bone. Tooth dimensions viii were measured and 3D bone morphometry was conducted. No statistically significant differences were found between the two mouse types. P2X7R does not have a major effect on alveolar bone or tooth morphology.The final part examines the role of the P2X7 receptor in a controlled biomechanical model. Orthodontic mechanotransduction was compared in wild-type (WT) and P2X7R knock-out (KO) mice. Using Finite Element Analysis, mouse mechanics were scaled to produce typical human stress levels. Relationships between the biological responses and the calculated stresses were statistically tested and compared.There were direct relationships between certain stress magnitudes and root resorption and bone formation. Hyalinization and root and bone resorption were different in WT and KO. Orthodontic responses are related to the principal stress patterns in the PDL and the P2X7 receptor plays a significant role in their mechanotransduction.

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Section: Orthodontic Mechanotransduction and The Role Of The P2x7 Recmentioning

confidence: 98%

Section: Biological Factors In Rate Of Tooth Movement and Errmentioning

confidence: 99%

Section: Development Of Biomechanical Orthodontic Models Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Orthodontic mechanotransduction and the role of the P2X7 receptor

Viecilli

Katona

Chen

et al. 2009

American Journal of Orthodontics and Dentofacial Orthopedics

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“…These mediators are bioactive molecules that regulate the inflammatory process. [2][3][4][5][6][7][8][9] The mechanism of bone remodeling can also be related to the release of inflammatory mediators, such as prostaglandin E 2 . [1][2][3][4] Prostaglandin E 2 (PGE 2 ) and interleukin IL-1β are key mediators involved in periodontal diseases, potent stimulators of bone resorption, and are produced by human periodontal ligament cells in response to mechanical stress.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9] The early phase of orthodontic tooth movement involves an acute inflammatory response characterized by local tissue ischemia, periodontal vasodilatation and the migration of leukocytes through the periodontal ligament capillaries. [1][2][3][4][5][6][7][8][9] In this process, endothelial cells are activated, and cytokines and chemoattractants are produced that result in leukocyte activation. The interaction between activated endothelial cells and leukocytes plays an important role in the inflammatory process by producing proinflammatory and anti-inflammatory cytokines.…”

Section: Introductionmentioning

confidence: 99%

Juveniles versus adults: differences in PGE2 levels in the gingival crevicular fluid during orthodontic tooth movement

2010

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Juveniles versus adults: differences in PGE 2 levels in the gingival crevicular fluid during orthodontic tooth movement Abstract: This study aimed to investigate age-related changes in the biosynthetic capacity of prostaglandin E 2 (PGE 2 ) in the gingival crevicular fluid (GCF) during one month of orthodontic treatment. Twentyfive juvenile subjects (mean age 13 ± 2.1 years) and 23 adults (mean age 24 ± 2.1 years) were included. GCF was collected immediately before the force application at the baseline, 2, 21 and 28 days, with periopaper inserted into the gingival crevice of the maxillary lateral incisors. The mediator levels were determined with an EIA kit. The results showed that the PGE 2 concentrations were significantly elevated from the baseline to 21 days (129.35 and 198.84 pg/µL, p = 0.0169) in juvenile subjects and reduced from 21 to 28 days (198.84 to 112.60 pg/µL, p = 0.0032). Adults, however, had no significant changes in the PGE 2 levels. The total amounts of PGE 2 from both groups changed between the baseline to 21 and 21 to 28 days (p = 0.0119 and p = 0.0076, respectively). The PGE 2 initial and final levels showed significant differences between the juveniles and adults, being higher in adults (baseline: juvenile = 129.35 pg/µL vs. adult = 163.20 pg/µL, p = 0.0379; t3: juvenile = 112.60 pg/µL and adult = 175.30 pg/µL, p = 0.0005). In conclusion, the results demonstrate the presence of variation in the PGE 2 levels according to age and the orthodontic activation period, which can explain why the speed of orthodontics treatment may be different in adults vs. juveniles.

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