Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

Barbosa, Hivana Patricia Melo; Martins, Luisa Carí­cio; Santos, Sidney Emanuel Batista dos; Demachki, Sâmia; Assumpção, Mônica Baraúna de; Aragão, Charliana; Corvelo, Tereza Cristina de Oliveira

doi:10.3748/wjg.15.1465

Cited by 54 publications

(39 citation statements)

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“…It is thus associated with an elevated risk of developing stomach cancer (25,29,33) . In Belém, PA, Brazil, a high rate of prevalence of infection by the bacteria has been described in adult patients with gastric disturbances (22,23) . Furthermore, the Northern Region of Brazil has a high frequency of gastric pathologies such as peptic ulcers and gastric adenocarcinoma (5) .…”

Section: Introductionmentioning

confidence: 99%

Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer

Vinagre

Corvelo

Arnaud

et al. 2011

Arq. Gastroenterol.

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-Context -Gastric neoplasia is the second most common cause of death by cancer in the world and H. pylori is classified as a type I human carcinogen by the World Health Organization. However, despite the high prevalence of infection by H. pylori around the world, less than 3% of individuals carrying the bacteria develop gastric neoplasias. Such a fact indicates that evolution towards malignancy may be associated with bacterial factors in the host and the environment. Objectives -To investigate the association between polymorphism in the region promoting the IL-8 (-251) gene and the H. pylori genotype, based on the vacA alleles and the presence of the cagA gene, using clinical and histopathological data. Methods -In a prospective study, a total of 102 patients with stomach cancer and 103 healthy volunteers were analysed. Polymorphism in interleukin 8 (-251) was determined by the PCRrestriction fragment length polymorphism reaction and sequencing. PCR was used for genotyping the vacA alleles and the cagA in the bacterial strains PCR. Gastric biopsies were histologically assessed. Results -The H. pylori serology was positive for 101 (99%) of all patients analysed, and 98 (97%) of them were colonized by only one strain. In patients with monoinfection, 82 (84%) of the bacterial strains observed had the s1b/m1 genotype. The cagA gene was detected in 74 (73%) of patients infected by H. pylori. The presence of the cagA gene was demonstrated as associated with the presence of the s1b/m1 genotype of the vacA gene (P = 0.002).As for polymorphism in the interleukin 8 (-251) gene we observed that the AA (P = 0.026) and AT (P = 0.005) genotypes were most frequent in the group of patients with gastric adenocarcinoma. By comparing the different types of isolated bacterial strains with the interleukin -8 (-251) and the histopathological data we observed that carriers of the A allele (AT and AA) infected by virulent strains (m1s1 cagA+) demonstrated a greater risk of presenting a degree of inflammation (OR = 24.75 CI 95% 2.29-267.20 P = 0.004) and increased neutrophilic activity (OR = 28.71 CI 95% 2.62-314 P = 0.002) in the gastric mucosa. Conclusion -Our results demonstrate that the interaction between polymorphism in the interleukin -8 (-251) gene, particularly with carriers of the A allele and the infecting type of H. pylori strain (s1m1 cagA positive) performs an important function in development of gastric adenocarcinoma.

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Section: Introductionmentioning

confidence: 99%

Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer

Vinagre

Corvelo

Arnaud

et al. 2011

Arq. Gastroenterol.

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“…An association of TNF-α -308G>A (rs1800629) with susceptibility/resistance to GC development has been widely investigated and yielded conflicting results. Studies including GC patients and controls from China (7)(8)(9)(10)(11)(12)(13)(14), Brazil (15,16), Portugal (17,18), United States (19), Poland (20), South Korea (21-23), Honduras (24), Italy (25), Colombia (26), and Japan (27) have been associated with susceptibility to GC development. However, other studies including those from India (28), Brazilian (29,30), Romania (31), Spain (32,33), South Korea (34,35), China (36), Mexico (37), Germany (38) and Finland (39) failed to show a possible link between the TNF-α (-308G>A) polymorphism and GC.…”

Section: Introductionmentioning

confidence: 99%

Role of TNF-α -308G/A gene polymorphism in gastric cancer risk: A case control study and meta-analysis

Du¹,

Gao²

2017

Turk J Gastroenterol

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Background/Aims: In the Chinese population, gastric cancer (GC) is ranked as the third most common type of cancer. Although the exact etiology of GC development is unclear, several factors, including genetic and environmental, have been identified as risk factors. Variations in cytokine genes and their receptors have been related to a higher risk of GC. A single nucleotide polymorphism in the promoter region of tumor necrosis factor-α (TNF-α) (-308G>A) has been associated with a higher risk of GC and in the present study we evaluated its possible association with GC in a Chinese cohort. In addition, we performed a meta-analysis to draw a firm conclusion about the association between TNF-α gene polymorphisms and GC. Materials and Methods: We enrolled 400 Chinese GC patients and matched healthy controls hailing from similar geographical areas. The TNF-α -308G/A polymorphism was genotyped by allele-specific polymerase chain reaction (AS-PCR). For the meta-analysis, earlier published articles were searched and eligible studies were included. Results: Prevalence of the heterozygous mutant (GA) and minor allele (A) were significantly higher in GC cases compared to healthy controls (GA: p<0.0001, odds ratio (OR)=4.90; A: p<0.0001, OR=2.84). A total of 36 eligible studies including the present report, encompassing of 8353 GC patients and 12099 controls, were analyzed for the meta-analysis. A significant association of the TNF-α polymorphism (-308G>A) with susceptibility to GC was only found in the Caucasian population (A vs G: p=0.001; AA vs GG: p=0.01; AG vs GG: p<0.0001; AA vs AG+GG: p=0.01; AA+AG vs p=0.003). Conclusion:The results of the present case control study and meta-analysis showed that associations between TNF-a variants with susceptibility to GC development is population and ethnic specific. Keywords: Gene polymorphism, TNF-α, gastric cancer, meta-analysis, association, Chinese Original ArticleCite this article as: Du LC, Gao R. Role of TNF-α -308G/A gene polymorphism in gastric cancer risk: a case control study and metaanalysis. Turk J Gastroenterol 2017; 28: 272-82.

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“…Este incremento en el riesgo puede ser explicado por el pH alto (pH = 6,6) entre los sujetos infectados por H. pylori y portadores del genotipo IL-1B-511TT en comparación con los portadores del genotipo IL-1B-511CC (pH = 2,4), lo cual conduce a un mayor daño de la mucosa 42 , y mayor riesgo de desarrollar cáncer gástrico 10 . El efecto sinérgico entre los polimorfi smos del gen IL-1B y la infección por H. pylori CagA+ ha sido reportado en varias poblaciones asiáticas 13,[42][43][44] , caucásicas 10,41 , y estudios en América latina 27,45,46 . Además, algunos autores han sugerido que la asociación sinérgica de estos SNPs con cáncer gástrico puede requerir de la presencia de H. pylori en la carcinogénesis gástrica 43,47 .…”

Section: Discussionunclassified

Asociación de los polimorfismos IL-1B-511 e IL-1RN y Helicobacter pylori CagA positivo con cáncer gástrico en una zona de riesgo alto en Colombia

et al. 2011

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Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population

Cited by 54 publications

References 34 publications

Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer

Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer

Role of TNF-α -308G/A gene polymorphism in gastric cancer risk: A case control study and meta-analysis

Asociación de los polimorfismos IL-1B-511 e IL-1RN y Helicobacter pylori CagA positivo con cáncer gástrico en una zona de riesgo alto en Colombia

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