Interleukin-1? and its type 1 receptor are expressed in developing neural circuits in the frog,Xenopus laevis

Jelaso, Anna M.; Acevedo, Sarah; Dang, Tina; Lepere, Annette; Ide, Charles F.

doi:10.1002/(sici)1096-9861(19980504)394:2<242::aid-cne8>3.0.co;2-2

Cited by 23 publications

(14 citation statements)

References 79 publications

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“…In this report, co‐localization of NGF and IL‐1β was assessed using double‐label ICC in 10 days post‐fertilization tadpoles ( N = 5), a developmental stage that showed robust expression of both IL‐1β and NGF (Jelaso et al, 1998). Double‐label ICC experiments showed that IL‐1β was present in almost all cell types and/or regions that showed NGF‐IR (listed in Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…5a). Additional cell types that expressed only IL‐1β are described in more detail in Jelaso et al (1998).…”

Section: Resultsmentioning

confidence: 99%

“…In a previous study, we showed that IL‐1β and the IL‐1R1 are co‐expressed in neurons that comprise early sensory‐motor circuits in the developing frog, Xenopus laevis (Jelaso et al, 1998). Among numerous cell types, IL‐1β and IL‐1R1 proteins were expressed in Mauthner's neuron, a long‐lived, identifiable neuron in the hindbrain.…”

Section: Introductionmentioning

confidence: 97%

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NGF and IL‐1β are co‐localized in the developing nervous system of the frog, Xenopus laevis

Jelaso

DeLong

2005

Intl J of Devlp Neuroscience

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NGF, a neurotrophic factor best known for its role in promoting cell survival, regulates many neurodevelopmental processes, including synaptic plasticity, neurite outgrowth and programmed cell death. Although there is a large amount of data regarding NGF in the developing nervous system of many species, there is little known about its regulation and role in the frog, Xenopus laevis. In this report, immunocytochemistry was used to characterize NGF protein expression in developing tadpoles. Protein expression was analyzed in tadpoles from stage 44/45 through stage 50, a period of development characterized by extensive neurite outgrowth, neuronal differentiation and an initial period of programmed cell death. Similar to other species, NGF was expressed in sensory cells and tissues, including the inner ear, eye, olfactory system, lateral line organs, papillae in the oral cavity, and gills tufts. In addition, NGF was expressed in specific cells in the central nervous system, cranial and dorsal root ganglia, spinal sensory and motoneurons, and muscle tissues in the tail and body cavity. In the mammalian nervous system, the cytokine, interleukin-1beta (IL-1beta) induces expression of NGF. In this report, double-label immunocytochemistry was used to determine the relationship between NGF and IL-1beta. Results showed most cell types and/or tissues that expressed NGF also expressed IL-1beta. However, NGF was typically associated with cellular and nuclear membranes, whereas IL-1beta appeared in the cytoplasm and nucleolus. The nuclear localization of IL-1beta supports the idea that it regulates gene transcription in the frog. The appearance of NGF and IL-1beta in the same cells suggests they may interact to influence neural development.

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Section: Resultsmentioning

confidence: 99%

“…5a). Additional cell types that expressed only IL‐1β are described in more detail in Jelaso et al (1998).…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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NGF and IL‐1β are co‐localized in the developing nervous system of the frog, Xenopus laevis

Jelaso

DeLong

2005

Intl J of Devlp Neuroscience

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“…Nowadays it is known that several interleukins are expressed in normal embryonic cells at very early stages of development [28][29][30]; we have previously demonstrated that IL1b is expressed in the neuroepithelium of the embryonic spinal cord and stimulates proliferation and differentiation in these cells [31]. Moreover, receptors for IL-6 and IL-8 have been detected in numerous tissues and organs in human foetuses [32].…”

Section: Interleukin Expression and Function During Embryonic Developmentioning

confidence: 93%

Prenatal expression of interleukin 1β and interleukin 6 in the rat pituitary gland

et al. 2008

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a b s t r a c tIt is known that interleukin 1b (IL-1b) and interleukin 6 (IL-6) are expressed post-natally in normal and tumoral cells in the anterior pituitary, and that they play a role in both the liberation of different hormones and in the growth, proliferation and tumor formation of the pituitary gland. However, their expression and role during embryonic and fetal development remain unknown. We have performed an immunocytochemistry study of prenatal expression and distribution of IL-1b and IL-6 in isolated embryonic rat Rathke's pouch prior to birth, more specifically between 13.5 and 19.5 days p.c. Western-blot analysis carried out on 19.5-day p.c. embryos showed positive immunolabelling for IL-1b and IL-6. These interleukins were initially expressed simultaneously in the rostral and ventral portions of Rathke's pouch in 15.5-day p.c. embryos, and this expression progressed caudodorsally in later developmental stages, extending to most of the hypophysis before birth. The number of cells expressing these interleukins increased throughout this period: 48.22% of anterior pituitary cells expressed IL-6 in 19.5-day embryos, whilst IL-1b was positive in 39.8% of the cells. Moreover, we have demonstrated that some adenohypophyseal cells co-express both interleukins. Such findings represent the first step towards an understanding of the physiological role of these interleukins in anterior pituitary development.

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“…The supernatant was collected and centrifuged at 16 000 3 g for 30 min to separate the membrane fraction. The latter was resuspended in PBS and the membranebound receptor proteins were solubilized by incubating the membrane fraction with an equal volume of 16 mM CHAPS for 30 min at 48C, followed by ultracentrifugation at 150 000 3 g for 60 min [26]. The supernatant was assayed for total protein content (Bradford; Bio-Rad) and stored at À808C until Western blot analysis.…”

Section: Extraction Of Il1r1 Membrane Proteinmentioning

confidence: 99%

Interleukin 1 in Oviductal Tissues of Viviparous, Oviparous, and Ovuliparous Species of Amphibians1

Jantra

Bigliardi

Brizzi

et al. 2007

Biology of Reproduction

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In previous reports, we have shown that interleukin 1 (IL1), a cytokine associated with implantation in mice, is also expressed in reproductive tissues of viviparous squamate reptiles and cartilaginous fishes. In the present study, we investigated the expression of IL1B and its functional membrane receptor type I (IL1R1) in amphibians, a class of vertebrates that is characterized by different reproductive modes, including internal and external fertilization. In particular, we investigated the oviductal tissues of the aplacental viviparous Salamandra lanzai, the oviparous Triturus carnifex, and the ovuliparous Bufo bufo. In immunohistochemistry with anti-human IL1B and IL1R1 polyclonal antibodies we found that in S. lanzai, most cells in the uterine mucosa were immunoreactive for IL1B and IL1R1. In T. carnifex, IL1B and IL1R1 were present in ciliated luminal cells, and there was evidence of IL1B in glandular cells. In B. bufo, the expression of IL1B and IL1R1 was limited to the apical cytoplasm of the ciliated oviductal cells. Western blot analysis showed that a putative mature form of IL1B, similar to that seen in mammals, was present in the oviductal tissues of S. lanzai, whereas different forms, which probably correspond to an inactive pro-IL1B protein, were found in T. carnifex and B. bufo. A band that corresponded to the predicted 80-kDa human IL1R1 was found in S. lanzai and T. carnifex. Although the present study shows that IL1B and IL1R1 expression occurs in all reproductive modes, the differential expression patterns noted between ovuliparity and oviparity and viviparity may reflect the different roles of IL1 in the various reproductive modes.

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Interleukin-1? and its type 1 receptor are expressed in developing neural circuits in the frog,Xenopus laevis

Cited by 23 publications

References 79 publications

NGF and IL‐1β are co‐localized in the developing nervous system of the frog, Xenopus laevis

NGF and IL‐1β are co‐localized in the developing nervous system of the frog, Xenopus laevis

Prenatal expression of interleukin 1β and interleukin 6 in the rat pituitary gland

Interleukin 1 in Oviductal Tissues of Viviparous, Oviparous, and Ovuliparous Species of Amphibians1

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