Interlaboratory Validation of the in vitro Eye Irritation Tests for Cosmetic Ingredients. (4) Haemoglobin Denaturation Test

Hatao, Masato; Murakami, Noriko; Sakamoto, Kazutami; Ohnuma, M.; Matsushige, C.; Kakishima, Hiroshi; Ogawa, Tadatomo; Kojima, Hiroshi; Matsukawa, K.; Masuda, K.; Chiba, Kenichi; Yoshizawa, Kazuhiko; Kaneko, Toyozo; Iwabuchi, Yoshimi; Matsushima, Yuko; Momma, Junko; Ohno, Yasuo

doi:10.1016/s0887-2333(98)00067-8

Cited by 11 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this, we initially standardised the in vitro test, since, previously, haemolysis and protein denaturation have generally been assessed under different experimental conditions (21,(24)(25)(26)(27)(28)(29)(30). From the various procedures described in the literature, we selected the one recommended by Pape et al (20), which evaluates the cytotoxicities of tensoactive agents and of products containing these substances, based on an assessment of their ability to cause haemolysis, combined with the determination of any damage caused to cell proteins released during the haemolysis.…”

Section: Discussionmentioning

confidence: 99%

A Reassessment of the In Vitro RBC Haemolysis Assay with Defibrinated Sheep Blood for the Determination of the Ocular Irritation Potential of Cosmetic Products: Comparison with the In Vivo Draize Rabbit Test

Alves

Presgrave

et al. 2008

Altern Lab Anim

View full text Add to dashboard Cite

We examined the correlation between results obtained from the in vivo Draize test for ocular irritation and in vitro results obtained from the sheep red blood cell (RBC) haemolytic assay, which assesses haemolysis and protein denaturation in erythrocytes, induced by cosmetic products. We sought to validate the haemolytic assay as a preliminary test for identifying highly-irritative products, and also to evaluate the in vitro test as alternative assay for replacement of the in vivo test. In vitro and in vivo analyses were carried out on 19 cosmetic products, in order to correlate the lesions in the ocular structures with three in vitro parameters: (i) the extent of haemolysis (H50); (ii) the protein denaturation index (DI); and (iii) the H50/DI ratio, which reflects the irritation potential (IP). There was significant correlation between maximum average scores (MAS) and the parameters determined in vitro (r = 0.752-0.764). These results indicate that the RBC assay is a useful and rapid test for use as a screening method to assess the IP of cosmetic products, and for predicting the IP value with a high level of concordance (94.7%). The assay showed high sensitivity and specificity rates of 91.6% and 100%, respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

A Reassessment of the In Vitro RBC Haemolysis Assay with Defibrinated Sheep Blood for the Determination of the Ocular Irritation Potential of Cosmetic Products: Comparison with the In Vivo Draize Rabbit Test

Alves

Presgrave

et al. 2008

Altern Lab Anim

View full text Add to dashboard Cite

show abstract

“…The studies carried out by this method have some limitations: they can not be used in the evaluation of dyed substances, insoluble ingredients, strong acids [7,8]. If necessary, the proposed method can be used as an alternative one to the traditional methods for the determination of the skin irritation and effect on the mucous membrane of the eye, and as a method for the assessment of damaging effect level in the study of toxicity of the surfactants and agents based on them.…”

Section: Subjectsmentioning

confidence: 99%

“…The analysis of study results shows that there is no always clear inverse relationship between the hemolysis rate of erythrocytes and the rate of denaturation ability (higher hemolysis ratelower denaturation rate), it means that the effect on protein structures and cell membrane in different surfactants may take place by different mechanisms of the effect depending on the chemical structure of substances [7,8].…”

mentioning

confidence: 99%

Suspension Culture of Erythrocytes in the Assessment of the Detergent Functional Component Toxicity Level

Yalovenko¹,

Raietska²,

Holichenkov³

et al. 2020

Innov Biosyst Bioeng

View full text Add to dashboard Cite

Background. The study is devoted to the assessment of the cytotoxic properties of various surfactants by a method that is highly sensitive to identification of their negative effect on living mammalian cells. Objective. The aim of the work is to carry out a comparative assessment of modern surfactants in terms of the degree of manifestation of cytotoxic properties. Methods. The study of 10 surfactants was carried out with a new method for assessing the cytotoxic effect of agents based on surfactants, which is a modification of The RBC Hemolysis Test and Hemoglobin Denaturation Test. This method calculates the integral cytotoxicity index, which takes into account the data obtained by two tests. The following indicators were determined in the work: the percentage of hemoglobin denaturation in a 1% surfactant solution (D, %), the concentration of the surfactant solution at which hemolysis of 50% erythrocytes occurs (H 50 ,%), and the cytotoxicity coefficient (C c , conv. un.) of surfactants which is calculated by the formula C c = H 50 /D10000. Results. According to the integral C c indicator, the studied surfactants can be ranked according to the degree of toxicity reduction in the following order: sodium lauryl sulfate (0.09)-sodium laurate sulfate (0.13)cocamidopropyl betaine (0.27)-sodium salt of polyethoxysulfosuccinate (0.27)-alkyldimethyl betaine (1.07)disodium cocoamphodiacetate (1.99)-sodium salt n-palmethylglutamic acid (3.22)-cocoglucoside (27.86). Diethanolamides of coconut oil fatty acids and polyquaternium 7 in the studied concentration range (up to 1%) did not show denaturation properties. Conclusions. Studies have shown that surfactants significantly differ from each other by the level of the damaging effect, the most aggressive components were anionic and amphoteric surfactants, nonionic surfactants have a significantly lower cytotoxic effect (10 times). The results obtained should be taken into account when creating soft agents based on surfactants.

show abstract

“…Other modifications of the method have evaluated HD by measuring both the optical density at 418 nm and the maximum absorption wavelength. The HD was then compared with that of a positive control and expressed according to three indices: the test substance concentration that induces 50% of the HD induced by the positive control; the relative HD rate induced by 1% of the test substance; and the change in the maximal absorption wavelength caused by 1% of the test substance 36. This method should not be applied to coloured materials if the absorption of the material is strong at around 418 nm.…”

Section: Alternatives To the Eye Irritation Testmentioning

confidence: 99%

Alternative Methods for Eye and Skin Irritation Tests: An Overview

Vinardell

Mitjans

2008

Journal of Pharmaceutical Sciences

162

View full text Add to dashboard Cite

Interlaboratory Validation of the in vitro Eye Irritation Tests for Cosmetic Ingredients. (4) Haemoglobin Denaturation Test

Cited by 11 publications

References 8 publications

A Reassessment of the In Vitro RBC Haemolysis Assay with Defibrinated Sheep Blood for the Determination of the Ocular Irritation Potential of Cosmetic Products: Comparison with the In Vivo Draize Rabbit Test

A Reassessment of the In Vitro RBC Haemolysis Assay with Defibrinated Sheep Blood for the Determination of the Ocular Irritation Potential of Cosmetic Products: Comparison with the In Vivo Draize Rabbit Test

Suspension Culture of Erythrocytes in the Assessment of the Detergent Functional Component Toxicity Level

Alternative Methods for Eye and Skin Irritation Tests: An Overview

Contact Info

Product

Resources

About