Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917

Sivaganesan, Mano; Willis, Jessica R.; Karim, Mohammad A.; Babatola, Akin; Catoe, David; Boehm, Alexandria B.; Wilder, Maxwell L.; Green, Hyatt; Lobos, Aldo; Harwood, Valerie J.; Hertel, Stephanie; Klepikow, Regina; Howard, Mondraya F.; Laksanalamai, Pongpan; Roundtree, Alexis; Mattioli, Mia; Eytcheson, Stephanie A.; Molina, Marirosa; Lane, Molly J.; Rediske, Richard R.; Ronan, Amanda; Dsouza, Nishita; Rose, Joan B.; Shrestha, Abhilasha; Hoar, Catherine; Silverman, Andrea I.; Faulkner, Wyatt; Wickman, Kathleen; Kralj, Jason G.; Servetas, Stephanie L.; Hunter, Monique E.; Jackson, Scott A.; Shanks, Orin C.

doi:10.1016/j.watres.2022.119162

Cited by 4 publications

(1 citation statement)

References 37 publications

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“…Many of these methods have been subjected to multiple laboratory performance assessments and shown to be highly reproducible when standardized protocols are used (Ebentier et al 2013 , Shanks et al 2016 ). Some human-associated qPCR assays are even available as government agency standardized protocols (USEPA 2019a , b ) with certified companion reference materials (Kralj et al 2021 , Sivaganesan et al 2022 , Willis et al 2022 ).…”

Section: Background Information On Genetic Targets and Methods: A His...mentioning

confidence: 99%

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

Self Cite

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The impacts on faecal pollution analysis using nucleic acid-based methods, such as PCR and sequencing, in health-related water quality research were assessed by rigorous literature analysis. A wide range of application areas and study designs has been identified since the first application more than 30 years ago (>1,100 publications). Given the consistency of methods and assessment types, we suggest defining this emerging part of science as a new discipline: genetic faecal pollution diagnostics (GFPD) in health-related microbial water quality analysis. Undoubtedly, GFPD has already revolutionised faecal pollution detection and microbial source tracking, the current core applications. GFPD is also expanding to many other research areas, including infection and health risk assessment, evaluation of microbial water treatment, and support of wastewater surveillance. In addition, storage of DNA extracts allows for biobanking, which opens up new perspectives. The tools of GFPD can be combined with cultivation-based standardised faecal indicator enumeration, pathogen detection, and various environmental data types, in an integrated data analysis approach. This comprehensive meta-analysis provides the scientific status quo of this field, including trend analyses and literature statistics, outlining identified application areas, and discussing the benefits and challenges of nucleic acid-based analysis in GFPD.

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Section: Background Information On Genetic Targets and Methods: A His...mentioning

confidence: 99%

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat

Paar,

Willis,

Sette

et al. 2024

Science of The Total Environment

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Quantitative Fecal Source Characterization of Urban Municipal Storm Sewer System Outfall ‘Wet’ and ‘Dry’ Weather Discharges

Shanks,

Diedrich,

Sivaganesan

et al. 2024

Water Research

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Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917

Cited by 4 publications

References 37 publications

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat

Quantitative Fecal Source Characterization of Urban Municipal Storm Sewer System Outfall ‘Wet’ and ‘Dry’ Weather Discharges

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