Interlaboratory Comparison Test as an Evaluation of Applicability of an Alternative Edible Oil Analysis by 1H NMR Spectroscopy

Abstract

A proton (1H) NMR spectroscopic method was established for the quality assessment of vegetable oils. To date, several research studies have been published demonstrating the high potential of the NMR technique in lipid analysis. An interlaboratory comparison was organized with the following main objectives: (1) to evaluate an alternative analysis of edible oils by using 1H NMR spectroscopy; and (2) to determine the robustness and reproducibility of the method. Five different edible oil samples were analyzed by evaluating 15 signals (free fatty acids, peroxides, aldehydes, double bonds, and linoleic and linolenic acids) in each spectrum. A total of 21 NMR data sets were obtained from 17 international participant laboratories. The performance of each laboratory was assessed by their z-scores. The test was successfully passed by 90.5% of the participants. Results showed that NMR spectroscopy is a robust alternative method for edible oil analysis.

31P NMR Method for Phospholipid Analysis in Krill Oil: Proficiency Testing—A Step toward Becoming an Official Method

“…Participation from Germany, Denmark, Norway, France, and the United States reflected the international interest and importance of analyzing the PL composition in krill oils by 31 P NMR spectroscopy. Previous successful NMR interlaboratory tests used the 1 H NMR technique and included untargeted metabolomics of biological samples and plant metabolomics data as well quality control of vegetable and fish oils (Igarashi et al, ; Martin et al, ; Piccinonna et al, ; Ward et al, ; Zailer et al, ). In general, the results of our study showed that the 31 P NMR method was precise and reproducible regarding quantitative analysis of the six specific PL species in krill oil.…”

A Comprehensive Study for Determination of Free Fatty Acids in Selected Biological Materials: A Review

“…The usage of 0.6 mL of CDCl 3 /DMSO-d 6 , CCl 4 /DMSO-d 6 , or CS 2 /DMSO-d 6 /CHCl 3 solvent mixtures in a 20 mg sample might assist in altering separation on the signals. The FFA value of the VOs and fruit oil could be easily detectable in the range of 2.2–2.4 ppm in the form of triplets, and carboxyl content could be measurable from 11 to 12 ppm in the form of a multiplet [ 8 , 95 , 96 ], whereas a strong signal could cause overlapping in this method [ 8 , 14 ]. For the acidic property determination of the VO sample, the 1 H NMR method presents a spectral width of 24 ppm, number of scans of 32, relaxation delay of 1 s, and acquisition time of 5.45 s [ 97 ].…”

Composition Profiling and Authenticity Assessment of Camellia Oil Using High Field and Low Field 1H NMR

“…The spectral peaks of CA and OL in the HF-NMR spectra were assigned according to the published literature [ 32 , 46 , 47 ]. Each spectrum was then binned into 2500 buckets across the range of δ5.5–0.5.…”