Interlaboratory Comparison of Autoradiographic DNA Profiling Measurements. 2. Measurement Uncertainty and Its Propagation

Duewer, David L.; Currie, Lloyd A.; Reeder, Dennis J.; Leigh, Stefan D.; Liu, Hung-Kung; Mudd, James L.

doi:10.1021/ac00103a013

Cited by 23 publications

(19 citation statements)

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“…Over the past decade, our group at NIST has been involved with a series of interlaboratory studies to help forensic DNA typing laboratories assess their performance with various technologies and issues relevant to forensic testing (10)(11)(12)(13)(14)(15)(16)(17). The most recent of these studies indicated that the accuracy of DNA quantitation does impact the quality of STR typing, particularly when examining mixture samples (16,17).…”

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confidence: 99%

Results from the NIST 2004 DNA Quantitation Study

Kline¹,

Duewer²,

Redman³

et al. 2005

Journal of Forensic Sciences

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For optimal DNA short tandem repeat (STR) typing results, the DNA concentration ([DNA]) of the sample must be accurately determined prior to the polymerase chain reaction (PCR) amplification step in the typing process. In early 2004, the National Institute of Standards and Technology (NIST) conducted an interlaboratory study to help assess the accuracy of DNA quantitation in forensic DNA laboratories. This study was designed with four primary purposes: (1) to examine concentration effects and to probe performance at the lower DNA concentration levels that are frequently seen in forensic casework; (2) to examine consistency with various methodologies across multiple laboratories; (3) to examine single versus multiple source samples; and (4) to study DNA stability over time and through shipping in two types of storage tubes. Eight DNA samples of [DNA] from 0.05 ng/µL to 1.5 ng/µL were distributed. A total of 287 independent data sets were returned from 80 participants. Results were reported for 19 different DNA quantitation methodologies. Approximately 65% of the data were obtained using traditional slot blot hybridization methods; 21% were obtained using newly available quantitative real-time PCR (Q-PCR) techniques. Information from this interlaboratory study is guiding development of a future NIST Standard Reference Material for Human DNA Quantitation, SRM 2372.

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confidence: 99%

Results from the NIST 2004 DNA Quantitation Study

Kline¹,

Duewer²,

Redman³

et al. 2005

Journal of Forensic Sciences

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“…While developing physical and chemical standards, the NIST has also conducted extensive interlaboratory validation trials to characterize performance of DNA markers and to improve analytical methods in forensic analysis and human genetic identification (23)(24)(25)(26)(27)(28)(29). This activity will be expanded as the NIST works with the National Cancer Institute (NCI) on collaborative validation projects.…”

Section: Standards For Forensic Analysis At the Nistmentioning

confidence: 99%

NIST physical standards for DNA‐based medical testing

Barker¹,

Watson²,

Ticehurst³

et al. 2002

Clinical Laboratory Analysis

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As DNA and RNA become major targets for clinical laboratory analysis, benchmark reagents will play an increasingly important role in standardization. Reliable national and international nucleic acid standards promote automation and third-party reimbursement for clinical testing. Furthermore, nucleic acid standards provide materials for quality assurance and quality control (QA/QC), and proficiency testing. Standard methods and training initially evolved from consensus guidelines endorsed by professional societies and governmental agencies. The National Institute of Standards and Technology (NIST), a nonregulatory agency of the U.S. Department of Commerce, develops and certifies physical and chemical standards in support of national commerce, manufacturing, and science. In its role supporting U.S. science and industry, the NIST responds to specific standards needs, most recently for medically and biologically important analytes. Broad-based consensus developed through interdisciplinary NIST workshops initiated development of NIST-certified DNA standards. Such materials serve the diagnostic community and help manufacturers benchmark a variety of DNA diagnostic testing platforms. Here we summarize the NIST experience and programs for development of national standards for DNA-based medical diagnostic testing.

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“…Indeed, analysis of memory should include behavioral tasks, type of memory, the dynamic hierarchy of neural markers and brain areas involved in memory formation (e.g., Euston et al, 2012 ; Eskenazi et al, 2015 ) vs. no training, amnesia, anti-amnesic effects or forgetting (e.g., see below). Likewise, the species and the nature of behavioral task (e.g., appetitively or aversively motivated), curves of behavioral acquisition (i.e., multi-trial or two trials task) or patterns of behavioral responses (progressive vs. all or none response), cognitive demand (easy or difficult task), timing of drug administration (pre-training, post-training or pretest) and kind of drug (e.g., agonist or antagonist), protocols of training and testing together with neurobiological markers (e.g., Duewer et al, 1995 ; Patton, 1995 ) accompanying mnemonic processes deserve attention. Among the behavioral memory tasks available (e.g., Peele and Vincent, 1989 ; Myhrer, 2003 ; Lynch, 2004 ); importantly, the implementation of new instruments for measuring memory in behavioral tasks assists in gaining deeper insight into learning and memory processes (e.g., Cook et al, 2004 ; Walker et al, 2011 ; Markou et al, 2013 ; Leger et al, 2014 ; Wolf et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Serotonin, neural markers, and memory

Meneses¹

2015

Front. Pharmacol.

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Diverse neuropsychiatric disorders present dysfunctional memory and no effective treatment exits for them; likely as result of the absence of neural markers associated to memory. Neurotransmitter systems and signaling pathways have been implicated in memory and dysfunctional memory; however, their role is poorly understood. Hence, neural markers and cerebral functions and dysfunctions are revised. To our knowledge no previous systematic works have been published addressing these issues. The interactions among behavioral tasks, control groups and molecular changes and/or pharmacological effects are mentioned. Neurotransmitter receptors and signaling pathways, during normal and abnormally functioning memory with an emphasis on the behavioral aspects of memory are revised. With focus on serotonin, since as it is a well characterized neurotransmitter, with multiple pharmacological tools, and well characterized downstream signaling in mammals' species. 5-HT1A, 5-HT4, 5-HT5, 5-HT6, and 5-HT7 receptors as well as SERT (serotonin transporter) seem to be useful neural markers and/or therapeutic targets. Certainly, if the mentioned evidence is replicated, then the translatability from preclinical and clinical studies to neural changes might be confirmed. Hypothesis and theories might provide appropriate limits and perspectives of evidence.

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Interlaboratory Comparison of Autoradiographic DNA Profiling Measurements. 2. Measurement Uncertainty and Its Propagation

Cited by 23 publications

References 0 publications

Results from the NIST 2004 DNA Quantitation Study

Results from the NIST 2004 DNA Quantitation Study

NIST physical standards for DNA‐based medical testing

Serotonin, neural markers, and memory

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