1976
DOI: 10.1126/science.935875
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Interkingdom Fusion Between Human (HeLa) Cells and Tobacco Hybrid (GGLL) Protoplasts

Abstract: The fusion of human HeLa cells with tobacco protoplasts has been accomplished with the use of polyethylene glycol. The sequence from heterocellular adherence to heterokaryon formation has been followed with light microscopy and confirmed by autoradiographs of heterokaryons containing unlabeled tobacco nuclei and tritium-labeled HeLa nuclei. The HeLa nucleus retained its integrity in the tobacco cytoplasm up to 6 days after fusion.

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Cited by 55 publications
(17 citation statements)
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“…This finding is consistent with earlier accounts on the impact of the fusion medium on membrane fusion events, like exocytosis (15) and cell-cell fusion (16,17). It has been proposed that the electrofusion enhancement reached by using hypoosmotic fusion media is caused by spectrin denaturation (18,19).…”
Section: Resultssupporting
confidence: 82%
“…This finding is consistent with earlier accounts on the impact of the fusion medium on membrane fusion events, like exocytosis (15) and cell-cell fusion (16,17). It has been proposed that the electrofusion enhancement reached by using hypoosmotic fusion media is caused by spectrin denaturation (18,19).…”
Section: Resultssupporting
confidence: 82%
“…The eukaryote cell is clearly highly accommodating, judging by the production of viable human/tobacco somatic cell fusions (Jones et al, 1976). Two different genomes share the same cytoplasm in F1 hybrids and allopolyploids, though there can be a tendency for one genome to be lost; for example, in some Hordeum hybrids (Bennett et al, 1976).…”
Section: Discussionmentioning
confidence: 99%
“…The method described by Jones et al (1976) was used for protoplasts fusion. Two antibiotics (100 U/ml steptomycin and 100 U/ml nystatin) were used to screen out the fusant and the sensitivities of three parental strains to these antibiotics were checked before the experiment.…”
Section: Protoplast Fusionmentioning
confidence: 99%