Interkingdom Fusion Between Human (HeLa) Cells and Tobacco Hybrid (GGLL) Protoplasts

Jones, C. E.; Mastrangelo, IA; Smith, H J; Liu, HZ; Meck, R. A.

doi:10.1126/science.935875

Cited by 55 publications

(17 citation statements)

References 11 publications

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“…This finding is consistent with earlier accounts on the impact of the fusion medium on membrane fusion events, like exocytosis (15) and cell-cell fusion (16,17). It has been proposed that the electrofusion enhancement reached by using hypoosmotic fusion media is caused by spectrin denaturation (18,19).…”

Section: Resultssupporting

confidence: 82%

Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion

Strömberg

Ryttsén

Chiu

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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Section: Resultssupporting

confidence: 82%

Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion

Strömberg

Ryttsén

Chiu

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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“…The eukaryote cell is clearly highly accommodating, judging by the production of viable human/tobacco somatic cell fusions (Jones et al, 1976). Two different genomes share the same cytoplasm in F1 hybrids and allopolyploids, though there can be a tendency for one genome to be lost; for example, in some Hordeum hybrids (Bennett et al, 1976).…”

Section: Discussionmentioning

confidence: 99%

The Japanese knotweed invasion viewed as a vast unintentional hybridisation experiment

Bailey

2012

Heredity

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Chromosome counts of plants grown from open-pollinated seed from Japanese knotweed around the world have revealed the presence of extensive hybridisation with both native and other introduced taxa. These hybrids fit into three categories: interand intraspecific hybrids involving the taxa of Fallopia section Reynoutria (giant knotweeds), hybrids between Japanese knotweed and F. baldschuanica (Regel) Holub and hybrids between Japanese knotweed and the Australasian endemics of the genus Muehlenbeckia. In this minireview, the viability of the different classes of hybrid and the potential threats they pose are discussed in the context of recent examples of allopolyploid speciation, which generally involve hybridisation between a native and an alien species. Such wide hybridisations also challenge accepted taxonomic classifications. Japanese knotweed s.l. provides a fascinating example of the interplay between ploidy level, hybridisation and alien plant invasion. The octoploid (2n ¼ 88) Fallopia japonica var. japonica (Houtt.) Ronse Decraene is a single female clone throughout much of its adventive range, and provides an ideal system for investigating the potential for wide hybridisation.

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“…The method described by Jones et al (1976) was used for protoplasts fusion. Two antibiotics (100 U/ml steptomycin and 100 U/ml nystatin) were used to screen out the fusant and the sensitivities of three parental strains to these antibiotics were checked before the experiment.…”

Section: Protoplast Fusionmentioning

confidence: 99%

Identification of protoplast fusion strain Fhhh by randomly amplified polymorphic DNA

Zhao

Zhang

et al. 2009

World J Microbiol Biotechnol

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A functional strain Fhhh was constructed through protoplast fusion of three parental strains (Phanerochaete chrysosporium, Saccharomyces cerevisiae and native bacterium YZ) to improve the degradation efficiency of purified terephthalic acid wastewater. Randomly amplified polymorphic DNA (RAPD) and scanning electron microscope (SEM) analysis were applied to identify and confirm the fusant Fhhh through phenotypic and genetic relationship. The result of SEM analysis demonstrated that the cell shape of fusant Fhhh differed from all three parental strains. RAPD analysis of 40 arbitrary primers generated a total of 1,135 bands. The genetic similarity indices between Fhhh and parental strains Phanerochaete chrysosporium (PC), Saccharomyces cerevisiae (SC) and native bacterium (YZ) were 34.01%, 33.16%, and 35.97%, respectively. The targeted-gene PCR results showed that Fhhh inherited the DNA fragments of mnp and lip genes from parental strain PC and FLO1 gene fragment from parental strain SC. Our results suggested protoplast fusion technique may be considered as a promising technique in environmental pollution control.

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Interkingdom Fusion Between Human (HeLa) Cells and Tobacco Hybrid (GGLL) Protoplasts

Cited by 55 publications

References 11 publications

Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion

Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion

The Japanese knotweed invasion viewed as a vast unintentional hybridisation experiment

Identification of protoplast fusion strain Fhhh by randomly amplified polymorphic DNA

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