Interisotypic α- and β-chain assembly as a source of HLA class-II diversity

Charron, Dominique; Hermans, Pierre; Lotteau, Vincent; Merlu, Benoit; Amesland, Françoise; Turmel, P; Tevton, L

doi:10.1007/bf02918182

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…The number of similar alleles can be extremely important, because it correlates best to the repertoire of MHC class I1 molecules at foeto-maternal interface. These heterodimeric molecules can be expressed in their cis-and trans-encoded isotypes (Charron et al, 1990). Since a heterozygous individual is able to express two different DR (the alpha chain of the DR molecule is not polymorphic) and four DQ and DP molecules, the number of different MHC class I1 molecules at the foeto-maternal interface can vary from 3 to 20.…”

Section: Foeto-maternal M H C Class Ii Compatibility Deduced From Parmentioning

confidence: 99%

Foeto‐maternal Compatibility in Hla‐dr, ‐dq, and ‐dp Loci in Finnish Couples Suffering From Recurrent Spontaneous Abortions

Laitinen

Koskimies

Westman

1993

Int J Immunogenetics

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The polymorphism of Major Histocompatibility Complex (MHC) class II genes DRB, DQA, DQB, and DPA was studied by TaqI Restriction Fragment Length Polymorphism (RFLP) in recurrent spontaneous abortions (RSA). The study group consisted of 35 primary abortion (PA) couples (no children) and 15 secondary abortion (SA) couples (1-2 children before abortions). We found no increase in DR-DQ compatibility between the mother and the foetus in the Finnish RSA group. In contrast to findings in some other populations, foeto-maternal incompatibility was increased in the PA group. Thus, our results do not support the theory that increased MHC class II compatibility is a cause of abortions as such. The Finns are a small and relatively isolated population with a unique gene inheritance. Thus, one can speculate that, if the human MHC class II is in the linkage with disadvantageous 'fertility genes', and these genes might nonetheless still be clustered in only a few MHC haplotypes among the Finns. This would be the reason, that DR-DQ sharing is not seen. The presence of rare HLA alleles, such as DR2 and DR6, among the aborters also supports this. In addition, this study extends our previous findings on MHC class III in regards to PA and SA couples differing immunogenetically from each other. In MHC class II, this was most obvious in the DPA1 locus. The vast majority of SA women were heterozygous for the two most common DPA1 alleles (14.0 kb and 13.5 kb), resulting in significantly smaller chances for a DPA1 mismatched foetus to occur in the SA group than in the controls or in the PA women.

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Section: Foeto-maternal M H C Class Ii Compatibility Deduced From Parmentioning

confidence: 99%

Foeto‐maternal Compatibility in Hla‐dr, ‐dq, and ‐dp Loci in Finnish Couples Suffering From Recurrent Spontaneous Abortions

Laitinen

Koskimies

Westman

1993

Int J Immunogenetics

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The role of DQ alpha–beta heterodimers in genetic susceptibility to insulin‐dependent diabetes

Deschamps

Khalil

1993

Diabetes Metab. Rev.

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Studies on the Interaction of T-Cells with Major Histocompatibility Complex Class II Antigens

Warrens

1997

Clinical Science

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1. Major histocompatibility complex class II antigens have the central role in the immune response of 'presenting' antigenic peptide to CD4+ T-cells. This interaction with a T-cell's receptor may result in activation, but, if recognition occurs without collateral molecular interactions which cause 'co-stimulation', these T-cells will be tolerized. 2. In the light of current interest in muscle cell transplantation, a transformed myoblast, TE671, phenotypically comparable to untransformed cells, transfected to express class II, was studied as a stable model of antigen presentation by muscle cells. These cells failed to activate T-cells but induced tolerance. 3. The DR alpha chain is unusual being the only non-polymorphic classical class II polypeptide, raising the question of its functional contribution. To this end, several single polypeptide constructs were generated with contributions from different class II alpha-chains. On this basis, it was established that DR alpha makes significant contributions to peptide binding and that its alpha 2 domain is also important in T-cell recognition, possibly through CD4 binding. 4. One implication of the lack of polymorphism of DR alpha may be that it has a wider range of pairing partners, possibly including beta chains of different isotypes. To address this, it is planned to use transfectants expressing only a mixed isotype pair to generate T-cell clones in vitro. These reagents would be useful tools to detect whether such mixed pairs exist physiologically. In this paper, the development of a system is described which will allow this question to be addressed.

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Interisotypic α- and β-chain assembly as a source of HLA class-II diversity

Cited by 3 publications

References 20 publications

Foeto‐maternal Compatibility in Hla‐dr, ‐dq, and ‐dp Loci in Finnish Couples Suffering From Recurrent Spontaneous Abortions

Foeto‐maternal Compatibility in Hla‐dr, ‐dq, and ‐dp Loci in Finnish Couples Suffering From Recurrent Spontaneous Abortions

The role of DQ alpha–beta heterodimers in genetic susceptibility to insulin‐dependent diabetes

Studies on the Interaction of T-Cells with Major Histocompatibility Complex Class II Antigens

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