Interindividual Variations in the Disomy Frequencies of Human Spermatozoa and their Correlation with Nuclear Maturity as Evaluated by Aniline Blue Staining

Morel, Frédéric; Mercier, Sylvie; Roux, Christophe; Elmrini, Tariq; Clavequin, Marie Claire; Bresson, Jean Luc

doi:10.1016/s0015-0282(98)00058-2

Cited by 38 publications

(27 citation statements)

References 18 publications

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“…Our data are in discordance with the data found by Morel et al [20] and Rives et al [21]. Indeed, Morel et al [20] have conducted a study on human semen samples randomly selected from a population of men undergoing an infertility work up and did not established a correlation between subject age and disomy frequencies. Rives et al [21] also confirmed that the disomy frequency is not correlated to subject age.…”

Section: Discussioncontrasting

confidence: 99%

“…Our results are in accordance with the data of many other studies that reported significant negative correlations between sperm concentration and the mean frequency of disomy, especially for the sex chromosomes [6,10,15,21,22]. However, other studies did not show a significant correlation between sex disomy rates and sperm concentration [3,20,23,24]. The disparate results of these studies might be related to their investigation of different types of male infertility: in the majority of cases oligozoospermia was associated with one or more abnormal other parameters.…”

Section: Discussionsupporting

confidence: 90%

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Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Mougou-Zerelli

Brahem

Kammoun

et al. 2011

J Assist Reprod Genet

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Purpose To evaluate the frequency of sperm nuclei disomy for chromosomes 8, X, and Y in patients with severe nonobstructive oligozoospermia and to assess possible correlations between sperm nuclei aneuploidy and semen parameters or a particular clinical phenotype. Materials and methods The sperm aneuploidy rate for chromosomes X, Y, and 8 were assessed in 16 infertile men with severe non-obstructive oligozoospermia and 7 healthy men with normal semen parameters. The frequency of sperm aneuploidy was compared between several patients groups according to their clinical and biological factors. Results The total rate of chromosomally abnormal spermatozoa was significantly higher in patients with severe oligozoospermia compared to control group (P<0.001). A significant relationship was found between the age of patients, sperm concentration, and morphology and the mean rate of sex chromosomes disomy. In addition to the low sperm count (<5×10 6 /ml), an elevated FSH level and an exposed to an elevated temperature are two major predictive factors leading to the production of higher numbers of chromosomally abnormal gametes. Conclusion Patients with severe oligozoospermia, who are potential candidates for assisted reproduction technology, presented a high level of sex numerical chromosome abnormalities, and consequently are at high risk of chromosome abnormalities in their offspring.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 90%

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Mougou-Zerelli

Brahem

Kammoun

et al. 2011

J Assist Reprod Genet

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“…Multiple studies have reported an association between sperm chromatin compaction and abnormal sperm head morphology [61][62][63] and acrosome abnormalities [64]. Although the compaction of the nuclear chromatin and DNA integrity are two interdependent parameters [65,66], some studies have reported an association between them [67,68]. We hypothesize the loss of negative charge from the plasma membrane may be associated via an apoptosis-mediated pathway.…”

Section: Discussionmentioning

confidence: 89%

Optimization of microelectrophoresis to select highly negatively charged sperm

Lee¹,

Murphy²,

Aston³

et al. 2016

J Assist Reprod Genet

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Purpose The sperm membrane undergoes extensive surface remodeling as it matures in the epididymis. During this process, the sperm is encapsulated in an extensive glycocalyx layer, which provides the membrane with its characteristic negative electrostatic charge. In this study, we develop a method of microelectrophoresis and standardize the protocol to isolate sperm with high negative membrane charge. Methods Under an electric field, the percentage of positively charged sperm (PCS), negatively charged sperm (NCS), and neutrally charged sperm was determined for each ejaculate prior to and following density gradient centrifugation (DGC), and evaluated for sperm DNA damage, and histone retention. Subsequently, PCS, NCS, and neutrally charged sperm were selected using an ICSI needle and directly analyzed for DNA damage. Results When raw semen was analyzed using microelectrophoresis, 94 % were NCS. In contrast, DGC completely or partially stripped the negative membrane charge from sperm resulting PCS and neutrally charged sperm, while the charged sperm populations are increased with an increase in electrophoretic current. Following DGC, high sperm DNA damage and abnormal histone retention were inversely correlated with percentage NCS and directly correlated with percentage PCS. NCS exhibited significantly lower DNA damage when compared with control (P < 0.05) and PCS (P < 0.05). When the charged sperm population was corrected for neutrally charged sperm, sperm DNA damage was strongly associated with NCS at a lower electrophoretic current. Conclusion The results suggest that selection of NCS at lower current may be an important biomarker to select healthy sperm for assisted reproductive treatment.

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“…This hypothesis is rather unlikely and would probably not cause such large variations; and current estimations of disomy frequencies in control populations are approximately the same [7,11,19,21].…”

Section: Methodological Differences In Using Interphasic In Situ Hybrmentioning

confidence: 87%

Sex Chromosome Aneuploidies in Sperm of 47, Xyy Men

Morel¹,

Roux²,

Bresson³

1999

Archives of Andrology

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Interindividual Variations in the Disomy Frequencies of Human Spermatozoa and their Correlation with Nuclear Maturity as Evaluated by Aniline Blue Staining

Cited by 38 publications

References 18 publications

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia

Optimization of microelectrophoresis to select highly negatively charged sperm

Sex Chromosome Aneuploidies in Sperm of 47, Xyy Men

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