Intergeneric transfer of a partial genome and direct production of monosomic addition plants by microprotoplast fusion

Ramulu, K. S.; Dijkhuis, P.; Rutgers, E.; Blaas, J.; Krens, F. A.; Verbeek, Walter; Colijn-Hooymans, C. M.; Verhoeven, H. A.

doi:10.1007/s001220050130

Cited by 14 publications

(16 citation statements)

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“…Unfortunately, in most cases the hybrids gained much more donor DNA than desired, resulting in an aberrant morphology and partially sterilility (Kovtun et al 1993;McCabe et al 1993), although a stable genomic transfer through asymmetric protoplast fusion has also been reported (Binsfeld et al 2000). Furthermore, asymmetric protoplast fusion has been successfully used for transfer of one or few chromosomes, the production of specific addition lines and the introgression of genes between sexually incompatible species (Ramulu et al 1996;Binsfeld et al 2000;Wardrop et al 2004;Chen et al 2004). Highly asymmetric fertile hybrids have only occasionally been described (Vlahova et al 1997;Xia et al 2003).…”

Section: Discussionmentioning

confidence: 95%

“…This disadvantage might be avoided by asymmetric protoplast fusion, which is also called donor-recipient fusion. In this manner, only a part of the donor genome is transferred to a receptor protoplast (Ramulu et al 1996), reducing the number of undesirable traits incorporated into the receptor genome. For transferring partial genome or cytoplasm, the donor protoplast was usually irradiated with X-or gamma-rays (ionizing irradiation) prior to fusion.…”

Section: Introductionmentioning

confidence: 99%

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Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss)

Yang

Zhang

Jin

et al. 2007

Plant Cell Tiss Organ Cult

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Asymmetric somatic hybrids were obtained between Gossypium hirsutum Coker 201 and wild cotton G. klozschianum Anderss. An investigation on the effect of ultraviolet (UV) irradiation on donor protoplasts was carried out, and the lethal dose was determined to be 38.7 J cm À2 . We firstly screened the putative hybrids by the color of the calli produced, followed by morphological, cytological, and molecular analysis of putative hybrid plants. Most regenerated plants derived from fused protoplasts displayed a recipient-like morphology, while some showed an intermediate phenotype between Coker 201 and G. klozschianum. Chromosome numbers in these somatic hybrids ranged from 54 to 74. The hybrids were verified by random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR). Absence or co-existence of parents' genome DNA fragments was identified through molecular analysis. The heredity of cytoplasm was investigated by cleaved amplified polymorphic sequence (CAPS) analysis using mitochondrial and chloroplast universal primer pairs. The results indicated that recombination and rearrangements might have occurred in some regions of mitochondria (mt) and chloroplast (cp) DNA. To our knowledge, this is the first report about asymmetric protoplast fusion in cotton, and the hybrids obtained would be useful for breeding programs.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss)

Yang

Zhang

Jin

et al. 2007

Plant Cell Tiss Organ Cult

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“…The most powerful aspect of somatic hybridization is the aneuploid resource. The hybrids may also prove useful for the production of monosomic or disomic addition plants by microprotoplast fusion and recombinant lines after backcrossing (Ramulu et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion

et al. 2004

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Symmetric somatic hybrid plants between Gossypium hirsutum Coker 201 and G. klotzschianum were obtained through electrofusion. The fusion products were cultured in KM8P medium supplemented with 2.685 microM alpha-naphthaleneacetic acid and 0.465 microM kinetin, and the regenerated plants were morphologically, genetically, and cytologically characterized. Nuclear-DNA flow cytometric analysis revealed that the plants tested (31 of 40) had a relative DNA content close to the total DNA contents of the two parents. Subsequent genome DNA analysis using random amplified polymorphic DNA markers revealed 16 of 18 plants were true somatic hybrids. Cytological investigation of the metaphase root-tip cells of seven hybrids revealed there were 72-81 chromosomes in the hybrids, a value close to the expected 78 chromosomes. The morphology of the hybrids was distinct from that of the parents and from that of the regenerants from protoplasts of Coker 201. Somatic hybridization represents a potent and novel tool for transferring genomes of wild cottons containing economically important traits to cultivars in breeding programs. This is the first report on the regeneration of somatic hybrids via protoplast fusion in cotton.

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“…For successful application of somatic hybrids for improvement of cotton crops, backcrosses with the local cultivars will have to be performed to eliminate undesirable traits and sterility. The hybrids may also prove useful for the production of monosomic or disomic addition lines by microprotoplast fusion and recombinant lines after backcrossing (Ramulu et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Somatic hybrids between Gossypium hirsutum L. (4×) and G. davidsonii Kellog (2×) produced by protoplast fusion

et al. 2006

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Somatic hybrids were obtained from electrofused protoplasts derived from embryogenic suspension cultures of tetraploid cotton (G. hirsutum L. cv. Coker 201) and embryogenic callus of diploid wild cotton G. davidsonii. The regenerants were initially identified as hybrids by RAPD (random amplified polymorphic DNA) analysis. Subsequently, observation on chromosome counting, morphology and SSR (simple sequence repeat) confirmed the hybrid status. Cytological investigation of the metaphase root-tip cells of the regenerated plants revealed there were 74 to 84 chromosomes in the plants, close to the expected 78 chromosomes. SSR analysis revealed the regenerated plants contained specific genomic fragments from both fusion partners, further confirmed their hybridity. The morphology of the plants was intermediate between the two fusion partners. The regenerants were difficult to develop into mature plants because their roots browned and they wilted from the stem apex before forming 3 to 5 true leaves. The hybrid plants were transferred to soil by grafting in vitro onto rootstocks.

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Intergeneric transfer of a partial genome and direct production of monosomic addition plants by microprotoplast fusion

Cited by 14 publications

References 0 publications

Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss)

Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss)

Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion

Somatic hybrids between Gossypium hirsutum L. (4×) and G. davidsonii Kellog (2×) produced by protoplast fusion

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