Intergeneric Hybrid from Jatropha curcas L. and Ricinus communis L.: Characterization and Polyploid Induction

Premjet, Duangporn; Obeng, Abraham Kusi; Kongbangkerd, Anupan; Premjet, Siripong

doi:10.3390/biology8020050

Cited by 9 publications

(6 citation statements)

References 36 publications

(55 reference statements)

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“…When plants are identified for ploidy, they can be roughly screened using external morphology first, and morphological identification is a more common and simpler identification method for polyploidy. Generally, polyploid plants are larger than normal plants, with broad and thick leaves, thick stems, larger floral organs, and other morphological characteristics [12][13][14]. The gigantism of organs in the polyploid plants is an important characteristic, but plants with hypertrophied organs are not always polyploids, and not all polyploids are definitely hypertrophied.…”

Section: Ploidy Identification Of Begonia × Benariensis Polyploidsmentioning

confidence: 99%

Polyploid Induction and Identification of Begonia × benariensis

Xie,

Zhao,

Huang

et al. 2024

Horticulturae

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Begonia × benariensis series varieties have high ornamental and economic value and are excellent varieties for large-area group planting in gardens and green areas. At present, the B. × benariensis series varieties grown in China rely on foreign imports, and the price of imported germplasm resources is expensive, which greatly increases the cost of flower enterprises. In view of this, B. × benariensis (2n = 2x = 34) was used as the material, the callus was treated with colchicine to induce polyploid plants, and the polyploid plants were subjected to morphology, DNA content identification, and stomatal identification, as well as comparisons of the morphophysiological indexes of the plants. The results showed that the polyploid was better induced with the treatment of 0.05% colchicine for 4 h, and the induction rate was 46.67%. Among the mutagenized plants, there were 42 triploids (2n = 3x = 51) and 98 tetraploids (2n = 4x = 68). The stomatal density of diploid leaves was about 1.09 times that of the triploid and 1.67 times that of the tetraploid, the defense cells of the leaves increased gradually, and the stomatal density decreased; with the increase in ploidy, the height of the plants increased, while the crown width decreased; the maximum length and width of the leaves decreased, the color of the leaves became darker, and the chlorophyll content increased. This study provides abundant variation materials and technical support for the selection and breeding of new varieties of B. × benariensis in the future.

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Section: Ploidy Identification Of Begonia × Benariensis Polyploidsmentioning

confidence: 99%

Polyploid Induction and Identification of Begonia × benariensis

Xie,

Zhao,

Huang

et al. 2024

Horticulturae

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“…However, the cultivation of J. curcas is more complex compared to the closely related species Ricinus communis L. (2n = 2x = 20). Intergeneric hybrids (i.e., J. curcas × R. communis) were obtained to evaluate their agronomic value [258]. Immature hybrid embryos were rescued in vitro, and the hybridity nature of plantlets was confirmed using RAPD markers.…”

Section: Dicot Speciesmentioning

confidence: 99%

“…The calli of intergeneric hybrids were obtained in vitro from the young stems using colchicine to induce polyploidy cells. Appropriately, the authors suggested that a high percentage of tetraploid cells (>50%) was crucial in overcoming the sterility problem following hybridization [258]. However, the production of amphidiploid plants has not been reported.…”

Section: Dicot Speciesmentioning

confidence: 99%

Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

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Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote the development of an immature or lethal embryo into a viable plant. Intraspecific, interspecific, or intergeneric crosses allow the introgression of important alleles of agricultural interest from wild species, such as resistance or tolerance to abiotic and biotic stresses or morphological traits in crops. However, pre-zygotic and post-zygotic reproductive barriers often present challenges in achieving successful hybridization. Pre-zygotic barriers manifest as incompatibility reactions that hinder pollen germination, pollen tube growth, or penetration into the ovule occurring in various tissues, such as the stigma, style, or ovary. To overcome these barriers, several strategies are employed, including cut-style or graft-on-style techniques, the utilization of mixed pollen from distinct species, placenta pollination, and in vitro ovule pollination. On the other hand, post-zygotic barriers act at different tissues and stages ranging from early embryo development to the subsequent growth and reproduction of the offspring. Many crosses among different genera result in embryo abortion due to the failure of endosperm development. In such cases, ER techniques are needed to rescue these hybrids. ER holds great promise for not only facilitating successful crosses but also for obtaining haploids, doubled haploids, and manipulating the ploidy levels for chromosome engineering by monosomic and disomic addition as well substitution lines. Furthermore, ER can be used to shorten the reproductive cycle and for the propagation of rare plants. Additionally, it has been repeatedly used to study the stages of embryonic development, especially in embryo-lethal mutants. The most widely used ER procedure is the culture of immature embryos taken and placed directly on culture media. In certain cases, the in vitro culture of ovule, ovaries or placentas enables the successful development of young embryos from the zygote stage to maturity.

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“…Inducing polyploidy in Jatropha is a widely employed strategy to produce larger seeds for increased biofuel production (de Oliveira et al 2013;Niu et al 2016;Premjet et al 2019). Some polyploid Jatropha lines have vegetative parts with different sizes and characteristics as well as different fruits and seeds due to changes in their genetic material.…”

Section: Transgenic Plant Characteristicsmentioning

confidence: 99%

Transformation of <i>Jatropha curcas</i> L. for production of larger seeds and increased amount of biodiesel

Wiluk

Enoki

Yusei

et al. 2021

Plant Biotechnology

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The development of green energy is important to mitigate global warming. Jatropha (Jatropha curcas L.) is a promising candidate for the production of alternative biofuel, which could reduce the burden on the Earth's resources. Jatropha seeds contain a large quantity of lipids that can be used to produce biofuel, and the rest of the plant has many other uses. Currently, techniques for plant genetic transformation are extensively employed to study, create, and improve the specific characteristics of the target plant. Successful transformation involves the alteration of plants and their genetic materials. The aim of this study was to generate Jatropha plants that can support biofuel production by increasing their seed size using genes found via the rice FOX-hunting system. The present study improved previous protocols, enabling the production of transgenic Jatropha in two steps: the first step involved using auxins and dark incubation to promote root formation in excised shoots and the second step involved delaying the timing of antibiotic selection in the cultivation medium. Transgenic plants were subjected to PCR analysis; the transferred gene expression was confirmed via RT-PCR and the ploidy level was investigated. The results suggest that the genes associated with larger seed size in Arabidopsis thaliana, which were found using the rice FOX-hunting system, produce larger seeds in Jatropha.

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Intergeneric Hybrid from Jatropha curcas L. and Ricinus communis L.: Characterization and Polyploid Induction

Cited by 9 publications

References 36 publications

Polyploid Induction and Identification of Begonia × benariensis

Polyploid Induction and Identification of Begonia × benariensis

Embryo Rescue in Plant Breeding

Transformation of <i>Jatropha curcas</i> L. for production of larger seeds and increased amount of biodiesel

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