Interferon λ3 gene (IL28B) is associated with spontaneous or treatment‐induced viral clearance in hepatitis C virus–infected multitransfused patients with thalassemia

Biswas, Aritra; Firdaus, Rushna; Gupta, Debanjali; Ghosh, Monika; Saha, Kallol; Chowdhury, Prosanto; Bhattacharyya, Maitree; Sadhukhan, Provash Chandra

doi:10.1111/trf.14070

Cited by 6 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies on the impact of IFNL4 polymorphisms in acute infection with HCV genotype 2 or 3 are rare, which may be explained by difficulties in identifying subjects early during infection to allow for detection of HCV RNA and genotyping. The association of absence of IFN-k4 (CC rs12979860 carriage) and spontaneous clearance among non-1 HCV genotypes has previously been reported in a study where the majority of enrolled patients had genotype 2 or 3 infection [17], and also in a cohort of patients with thalassemia and a predominance of acute HCV genotype 3 infection [17,18]. In the present study, PWID were longitudinally monitored at intervals of approximately 3 months leading to high precision of estimating the onset of HCV infection and also to a high HCV-RNA detection rate allowing for HCV genotyping in 121 of 139 participants from the Malm€ o cohort.…”

Section: Discussionmentioning

confidence: 68%

Absence of interferon-λ 4 enhances spontaneous clearance of acute hepatitis C virus genotypes 1-3 infection

Waldenström

Kåberg

Blomé

et al. 2021

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

Objectives: Absence of a functional interferon-k 4 (IFN-k4) gene (IFNL4) predicts spontaneous resolution of acute hepatitis C virus (HCV) infections in regions with a predominance of genotype 1, whereas variants of the inosine triphosphate pyrophosphatase (ITPase) gene (ITPA) entailing reduced activity associate with increased sustained virologic response rates following some therapeutic regimens. This study aimed at investigating the impact of IFNL4 on acute HCV genotype 2 or 3 infections, and whether ITPase activity influenced outcome. Materials and Methods: Two hundred and seven people who injected drugs (PWID) with documented anti-HCV seroconversion, and 57 PWID with reinfection with HCV were analyzed regarding IFNL4 (rs368234815 and rs12979860) and ITPA (rs1127354 and rs7270101), and longitudinally followed regarding HCV RNA. Results: The spontaneous clearance of HCV infection in anti-HCV seronegative PWID was enhanced when IFN-k4 was absent (44% vs. 20% for IFNL4 TT/TT rs1368234815 and DG rs1368234815 respectively, p < .001; OR 3.2) across genotypes 1-3. The proportion lacking IFN-k4 was further increased following resolution of repeated re-exposure to HCV (74% among re-infected participants who had cleared at least two documented HCV infections). ITPA genetic variants did not independently impact on the outcome, but among males lacking IFN-k4, reduced ITPase activity markedly augmented the likelihood of resolution (65% vs. 29% for <100% and 100% ITPase activity, p ¼ .006). Conclusions: Absence of IFN-k4 entails an enhanced likelihood of spontaneous resolution both following primary acute infection and repeated re-exposure to HCV across genotypes 1-3. Among men lacking IFN-k4, reduced ITPase activity improved outcome.

show abstract

Section: Discussionmentioning

confidence: 68%

Absence of interferon-λ 4 enhances spontaneous clearance of acute hepatitis C virus genotypes 1-3 infection

Waldenström

Kåberg

Blomé

et al. 2021

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

show abstract

“…The plans also included some commendations for ideal treatment regimes grounded on the patient's clinical history and genotype. Ruhl and Everhart defined that non-alcoholic steatohepatitis generally includes the danger of liver damage, which can range from mild fat accumulation between hepatocytes to complex non-alcoholic steatohepatitis that can progress towards the cirrhosis and liver failure 19 .…”

Section: Discussionmentioning

confidence: 99%

Assessing Responsiveness of Elevated Serum Ferritin Levels for Treatment in Patients with Chronic Hepatitis C Infection

Ullah¹,

Adeel²,

Iqbal³

et al. 2022

PJMHS

View full text Add to dashboard Cite

Background: The infection of HCV is recognized worldwide as an important community health problem. There is an optimistic correlation between accumulation of iron in hepatocytes and amplified serum levels of markers, counting ferritin and transferrin. Aim: The goal of this study is to determine the response of serum levels of ferritin after the patient’s treatment with HCV infection. Material and methods: The study included 140 HCV-infected patients. All subjects were evaluated for clinical topographies, including HCV viral load, status of serum iron levels and biochemical data. The obtained outcomes were calculated by means of Microsoft Excel 2013. Results: The results showed that treatment with antiviral drugs interfered significantly the iron deposition in hepatocytes and have association between iron accumulation and HCV treatment. The mean iron value was 20.02 µmol / L for the positive PCR patients; and for a negative PCR reaction, 25.11 µmol / L was the estimated value before treatment. Both values were lower the normal array of 9-31.3 µmol / L. It is estimated that the mean ferritin value for negative PCR (668.55 µmol / L) and positive PCR (913.14 µmol / L) is above normal range which is 221 to 641.35 µmol / L. Conclusions: Patients with chronic HCV are significantly related to serum iron levels. High levels of iron and ferritin were associated significantly with the disease, resulting progressive fibrosis in the liver. There is no considerable change in serum iron markers among subjects with increased or less severity of HCV infection. The severity of the disease can be evaluated easily by measuring the serum transferrin levels along with ferritin levels, which are linked with the progression of fibrosis of the liver and necro-inflammatory activity. Keywords: levels of serum ferritin; iron load; drug; liver cells and HCV infection.

show abstract

“…Briefly, viral RNA was extracted from 140μl serum, eluted in 50μl elution buffer and stored at -80ºC for further use. Detection of HCV viral RNA was done by nested RT-PCR based on 5´UTR of HCV genome as described previously (Saha et al, 2014) 17 . Briefly, first round one tube RT-PCR was done in 20μl total reaction volume containing 2μl isolated RNA and second round nested PCR was performed in 25μl total volume using 2μl of 1 st round RT-PCR product.…”

Section: Detection Of Hcv Rnamentioning

confidence: 99%

Study of Hepatitis C Virus infection among multi-transfused patients with inherited β-globin synthesis gene defect, in the eastern region of India

Dutta

Biswas

Choudhury

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Post transfusion acquired HCV infection is common in high risk group individuals such as b-thalassemia patients who depend on regular blood transfusions. This study was conducted to determine the prevalence, genotype distribution, transmission dynamics and correlation of patients age and gender with spontaneous clearance of HCV in multi-transfused b-thalassemia patients, in the state of West Bengal, India. HCV RNA was first detected, using RT-PCR, from serum samples collected from HCV seropositive b-thalassemic individuals over five years. Viral RNA was further sequenced, and genotyped, using NCBI genotyping tool, for phylogenetic and phylogeographic studies using MEGA-X and BEAST package 1.10.0, respectively. Out of 777 HCV seropositive b-thalassemic patients, 503 (64.73%) were positive for HCV RNA, while 274 (35.26%) had spontaneously cleared the virus. Female thalassemic patients and patients belonging to ages 10-14 years, had a higher chance of spontaneously clearing the virus. The most prevalent HCV genotype in this study population was 3a (77.61%) followed by 1b (11.94%). Phylogeographic analyses revealed that the 3a strains migrated from countries like Pakistan, China Myanmar and Thailand whereas the 1b strains transmitted from Bangladesh and Myanmar. The prevalence of HCV infection is very high among Indian b-thalassemic patients, which necessitates a critical look into the prevailing transfusion practices and requires implementation of more rigid donor screening criteria to decrease the rate of transfusion transmitted HCV infection, especially in the highly susceptible thalassemic patients. The use of more sensitive NAT based assays for HCV detection in donor blood is a compressing need of the hour

show abstract

Interferon λ3 gene (IL28B) is associated with spontaneous or treatment‐induced viral clearance in hepatitis C virus–infected multitransfused patients with thalassemia

Cited by 6 publications

References 25 publications

Absence of interferon-λ 4 enhances spontaneous clearance of acute hepatitis C virus genotypes 1-3 infection

Absence of interferon-λ 4 enhances spontaneous clearance of acute hepatitis C virus genotypes 1-3 infection

Assessing Responsiveness of Elevated Serum Ferritin Levels for Treatment in Patients with Chronic Hepatitis C Infection

Study of Hepatitis C Virus infection among multi-transfused patients with inherited β-globin synthesis gene defect, in the eastern region of India

Contact Info

Product

Resources

About