2000
DOI: 10.1620/tjem.192.127
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Interferon-γ Stimulates the Expression of CX3CL1/Fractalkine in Cultured Human Endothelial Cells

Abstract: CX3CL1/Fractalkine, a CX3C chemokine, is a potent agonist for the chemotaxis and adhesion of monocytes and lymphocytes. It was first identified as a membrane protein in endothelial cells activated with IL-1 or TNF-alpha. We have found the enhanced expression of fractalkine in human umbilical vein endothelial cells stimulated with interferon-gamma (IFN-gamma). Pretreatment of the cells with cycloheximide did not inhibit the expression of fractalkine mRNA. The majority of fractalkine protein was found in the cel… Show more

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Cited by 130 publications
(150 citation statements)
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(38 reference statements)
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“…WY14643 and ciglitazone were from Calbiochem (San Diego, CA). Primer oligo(dT) [12][13][14][15][16][17][18] and MMulv reverse transcriptase were from Gibco-BRL (Gainthersburg, MD). RNeasy total RNA isolation kit and Taq DNA polymerase were purchased from Qiagen (Hilden, Germany).…”
Section: Reagentsmentioning
confidence: 99%
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“…WY14643 and ciglitazone were from Calbiochem (San Diego, CA). Primer oligo(dT) [12][13][14][15][16][17][18] and MMulv reverse transcriptase were from Gibco-BRL (Gainthersburg, MD). RNeasy total RNA isolation kit and Taq DNA polymerase were purchased from Qiagen (Hilden, Germany).…”
Section: Reagentsmentioning
confidence: 99%
“…15,[23][24][25][26] Culture medium was Humedia EB-2 supplemented with 2% fetal bovine serum (FBS), 10 ng/mL recombinant human (r(h)) epidermal growth factor, 1 µ g/mL hydrocortisone, 5 ng/mL r(h) basic fibroblast growth factor and 10 µ g/mL heparin. When the cultures reached about 80% confluence, the medium containing growth factors was removed and the cells were cultured in Humedia EB-2 supplemented with 20% human serum (Humedia-HS).…”
Section: Cell Culturementioning
confidence: 99%
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